Impact of dynasore an inhibitor of dynamin II on Shigella flexneri infection.
Shigella flexneri remains a significant human pathogen due to high morbidity among children < 5 years in developing countries. One of the key features of Shigella infection is the ability of the bacterium to initiate actin tail polymerisation to disseminate into neighbouring cells. Dynamin II is...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3868620?pdf=render |
| _version_ | 1818921174558572544 |
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| author | Mabel Lum Stephen R Attridge Renato Morona |
| author_facet | Mabel Lum Stephen R Attridge Renato Morona |
| author_sort | Mabel Lum |
| collection | DOAJ |
| description | Shigella flexneri remains a significant human pathogen due to high morbidity among children < 5 years in developing countries. One of the key features of Shigella infection is the ability of the bacterium to initiate actin tail polymerisation to disseminate into neighbouring cells. Dynamin II is associated with the old pole of the bacteria that is associated with F-actin tail formation. Dynamin II inhibition with dynasore as well as siRNA knockdown significantly reduced Shigella cell to cell spreading in vitro. The ocular mouse Sereny model was used to determine if dynasore could delay the progression of Shigella infection in vivo. While dynasore did not reduce ocular inflammation, it did provide significant protection against weight loss. Therefore dynasore's effects in vivo are unlikely to be related to the inhibition of cell spreading observed in vitro. We found that dynasore decreased S. flexneri-induced HeLa cell death in vitro which may explain the protective effect observed in vivo. These results suggest the administration of dynasore or a similar compound during Shigella infection could be a potential intervention strategy to alleviate disease symptoms. |
| first_indexed | 2024-12-20T01:33:27Z |
| format | Article |
| id | doaj.art-f7024dc1667c4b5e94ab711161a8b896 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T01:33:27Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f7024dc1667c4b5e94ab711161a8b8962022-12-21T19:58:04ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01812e8497510.1371/journal.pone.0084975Impact of dynasore an inhibitor of dynamin II on Shigella flexneri infection.Mabel LumStephen R AttridgeRenato MoronaShigella flexneri remains a significant human pathogen due to high morbidity among children < 5 years in developing countries. One of the key features of Shigella infection is the ability of the bacterium to initiate actin tail polymerisation to disseminate into neighbouring cells. Dynamin II is associated with the old pole of the bacteria that is associated with F-actin tail formation. Dynamin II inhibition with dynasore as well as siRNA knockdown significantly reduced Shigella cell to cell spreading in vitro. The ocular mouse Sereny model was used to determine if dynasore could delay the progression of Shigella infection in vivo. While dynasore did not reduce ocular inflammation, it did provide significant protection against weight loss. Therefore dynasore's effects in vivo are unlikely to be related to the inhibition of cell spreading observed in vitro. We found that dynasore decreased S. flexneri-induced HeLa cell death in vitro which may explain the protective effect observed in vivo. These results suggest the administration of dynasore or a similar compound during Shigella infection could be a potential intervention strategy to alleviate disease symptoms.http://europepmc.org/articles/PMC3868620?pdf=render |
| spellingShingle | Mabel Lum Stephen R Attridge Renato Morona Impact of dynasore an inhibitor of dynamin II on Shigella flexneri infection. PLoS ONE |
| title | Impact of dynasore an inhibitor of dynamin II on Shigella flexneri infection. |
| title_full | Impact of dynasore an inhibitor of dynamin II on Shigella flexneri infection. |
| title_fullStr | Impact of dynasore an inhibitor of dynamin II on Shigella flexneri infection. |
| title_full_unstemmed | Impact of dynasore an inhibitor of dynamin II on Shigella flexneri infection. |
| title_short | Impact of dynasore an inhibitor of dynamin II on Shigella flexneri infection. |
| title_sort | impact of dynasore an inhibitor of dynamin ii on shigella flexneri infection |
| url | http://europepmc.org/articles/PMC3868620?pdf=render |
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