Transient Suppression of Dbx1 PreBötzinger Interneurons Disrupts Breathing in Adult Mice.
Interneurons derived from Dbx1-expressing precursors located in the brainstem preBötzinger complex (preBötC) putatively form the core oscillator for inspiratory breathing movements. We tested this Dbx1 core hypothesis by expressing archaerhodopsin in Dbx1-derived interneurons and then transiently hy...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5017730?pdf=render |
| _version_ | 1811201946624720896 |
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| author | Nikolas C Vann Francis D Pham John A Hayes Andrew Kottick Christopher A Del Negro |
| author_facet | Nikolas C Vann Francis D Pham John A Hayes Andrew Kottick Christopher A Del Negro |
| author_sort | Nikolas C Vann |
| collection | DOAJ |
| description | Interneurons derived from Dbx1-expressing precursors located in the brainstem preBötzinger complex (preBötC) putatively form the core oscillator for inspiratory breathing movements. We tested this Dbx1 core hypothesis by expressing archaerhodopsin in Dbx1-derived interneurons and then transiently hyperpolarizing these neurons while measuring respiratory rhythm in vitro or breathing in vagus-intact adult mice. Transient illumination of the preBötC interrupted inspiratory rhythm in both slice preparations and sedated mice. In awake mice, light application reduced breathing frequency and prolonged the inspiratory duration. Support for the Dbx1 core hypothesis previously came from embryonic and perinatal mouse experiments, but these data suggest that Dbx1-derived preBötC interneurons are rhythmogenic in adult mice too. The neural origins of breathing behavior can be attributed to a localized and genetically well-defined interneuron population. |
| first_indexed | 2024-04-12T02:31:33Z |
| format | Article |
| id | doaj.art-f70772f8ed2a4c60bc6c10aee2b61297 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T02:31:33Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f70772f8ed2a4c60bc6c10aee2b612972022-12-22T03:51:47ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01119e016241810.1371/journal.pone.0162418Transient Suppression of Dbx1 PreBötzinger Interneurons Disrupts Breathing in Adult Mice.Nikolas C VannFrancis D PhamJohn A HayesAndrew KottickChristopher A Del NegroInterneurons derived from Dbx1-expressing precursors located in the brainstem preBötzinger complex (preBötC) putatively form the core oscillator for inspiratory breathing movements. We tested this Dbx1 core hypothesis by expressing archaerhodopsin in Dbx1-derived interneurons and then transiently hyperpolarizing these neurons while measuring respiratory rhythm in vitro or breathing in vagus-intact adult mice. Transient illumination of the preBötC interrupted inspiratory rhythm in both slice preparations and sedated mice. In awake mice, light application reduced breathing frequency and prolonged the inspiratory duration. Support for the Dbx1 core hypothesis previously came from embryonic and perinatal mouse experiments, but these data suggest that Dbx1-derived preBötC interneurons are rhythmogenic in adult mice too. The neural origins of breathing behavior can be attributed to a localized and genetically well-defined interneuron population.http://europepmc.org/articles/PMC5017730?pdf=render |
| spellingShingle | Nikolas C Vann Francis D Pham John A Hayes Andrew Kottick Christopher A Del Negro Transient Suppression of Dbx1 PreBötzinger Interneurons Disrupts Breathing in Adult Mice. PLoS ONE |
| title | Transient Suppression of Dbx1 PreBötzinger Interneurons Disrupts Breathing in Adult Mice. |
| title_full | Transient Suppression of Dbx1 PreBötzinger Interneurons Disrupts Breathing in Adult Mice. |
| title_fullStr | Transient Suppression of Dbx1 PreBötzinger Interneurons Disrupts Breathing in Adult Mice. |
| title_full_unstemmed | Transient Suppression of Dbx1 PreBötzinger Interneurons Disrupts Breathing in Adult Mice. |
| title_short | Transient Suppression of Dbx1 PreBötzinger Interneurons Disrupts Breathing in Adult Mice. |
| title_sort | transient suppression of dbx1 prebotzinger interneurons disrupts breathing in adult mice |
| url | http://europepmc.org/articles/PMC5017730?pdf=render |
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