Anticonvulsant Effect of Alcea aucheri on Pentylenetetrazole and Maximal Electroshock Seizures in Mice
Introduction: This study was designed to investigate the possible anticonvulsant effect of acute administration of an aqueous extract of flowers of Alcea aucheri (EFA) in two in vivo seizure models. Methods: Seizures were induced in male adult Swiss mice by administration of pentylenetetrazol (PTZ)...
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| Format: | Article |
| Language: | English |
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Iran University of Medical Sciences
2020-05-01
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| Series: | Basic and Clinical Neuroscience |
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| Online Access: | http://bcn.iums.ac.ir/article-1-1605-en.html |
| _version_ | 1797281028581097472 |
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| author | Tajmah Mombeini Babak Asadpour Behzadi Ramtin Ejtemaei Freidoun Tahmasbi Mohammad Kamalinejad Ahmad Reza Dehpour |
| author_facet | Tajmah Mombeini Babak Asadpour Behzadi Ramtin Ejtemaei Freidoun Tahmasbi Mohammad Kamalinejad Ahmad Reza Dehpour |
| author_sort | Tajmah Mombeini |
| collection | DOAJ |
| description | Introduction: This study was designed to investigate the possible anticonvulsant effect of acute administration of an aqueous extract of flowers of Alcea aucheri (EFA) in two in vivo seizure models.
Methods: Seizures were induced in male adult Swiss mice by administration of pentylenetetrazol (PTZ) or Maximal Electroshock (MES). Mice were randomly subjected to receive saline, EFA (8.75-175 mg.kg-1), or diazepam intraperitoneally (i.p.) 15 or 30 min before PTZ injection. In another experiment, mice were treated (i.p.) with saline, EFA (8.75-350 mg.kg-1), or phenytoin 15 or 30 min before the MES test. Diazepam and phenytoin were used as reference drugs.
Results: EFA (175 mg.kg-1) significantly increased the PTZ-induced seizure threshold compared with the saline control group 15 min after its administration. In the MES test, the extract (35 mg.kg-1) increased the latency to onset of tonic Hind Limb Extension (HLE) (seizure activity) compared with the saline group 15 min after treatment. Also, 30 min after treatment, EFA (35, 70, and 175 mg.kg-1) increased the latency to onset of the seizure, decreased the duration of the seizure (70 mg.kg-1), and decreased seizure occurrence (350 mg.kg-1) compared with those of the saline group. At both time points, the extract at all doses significantly reduced the mortality rate compared with the saline group.
Conclusion: These findings provide evidence of a possible anticonvulsant effect of A. aucheri in PTZ and MES seizure models in mice |
| first_indexed | 2024-03-07T16:49:41Z |
| format | Article |
| id | doaj.art-f7096a13b95d4542af1d1bc3e3f528e1 |
| institution | Directory Open Access Journal |
| issn | 2008-126X 2228-7442 |
| language | English |
| last_indexed | 2024-03-07T16:49:41Z |
| publishDate | 2020-05-01 |
| publisher | Iran University of Medical Sciences |
| record_format | Article |
| series | Basic and Clinical Neuroscience |
| spelling | doaj.art-f7096a13b95d4542af1d1bc3e3f528e12024-03-03T05:35:54ZengIran University of Medical SciencesBasic and Clinical Neuroscience2008-126X2228-74422020-05-01113369378Anticonvulsant Effect of Alcea aucheri on Pentylenetetrazole and Maximal Electroshock Seizures in MiceTajmah Mombeini0Babak Asadpour Behzadi1Ramtin Ejtemaei2Freidoun Tahmasbi3Mohammad Kamalinejad4Ahmad Reza Dehpour5 Department of Pharmacology, School of Medicine, Shahed University, Tehran, Iran. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. School of Paramedics, Tehran Medical Branch, Islamic Azad University, Tehran, Iran. School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Introduction: This study was designed to investigate the possible anticonvulsant effect of acute administration of an aqueous extract of flowers of Alcea aucheri (EFA) in two in vivo seizure models. Methods: Seizures were induced in male adult Swiss mice by administration of pentylenetetrazol (PTZ) or Maximal Electroshock (MES). Mice were randomly subjected to receive saline, EFA (8.75-175 mg.kg-1), or diazepam intraperitoneally (i.p.) 15 or 30 min before PTZ injection. In another experiment, mice were treated (i.p.) with saline, EFA (8.75-350 mg.kg-1), or phenytoin 15 or 30 min before the MES test. Diazepam and phenytoin were used as reference drugs. Results: EFA (175 mg.kg-1) significantly increased the PTZ-induced seizure threshold compared with the saline control group 15 min after its administration. In the MES test, the extract (35 mg.kg-1) increased the latency to onset of tonic Hind Limb Extension (HLE) (seizure activity) compared with the saline group 15 min after treatment. Also, 30 min after treatment, EFA (35, 70, and 175 mg.kg-1) increased the latency to onset of the seizure, decreased the duration of the seizure (70 mg.kg-1), and decreased seizure occurrence (350 mg.kg-1) compared with those of the saline group. At both time points, the extract at all doses significantly reduced the mortality rate compared with the saline group. Conclusion: These findings provide evidence of a possible anticonvulsant effect of A. aucheri in PTZ and MES seizure models in micehttp://bcn.iums.ac.ir/article-1-1605-en.htmlalcea aucheripentylenetetrazoleseizure thresholdmaximal electroshock seizuremice |
| spellingShingle | Tajmah Mombeini Babak Asadpour Behzadi Ramtin Ejtemaei Freidoun Tahmasbi Mohammad Kamalinejad Ahmad Reza Dehpour Anticonvulsant Effect of Alcea aucheri on Pentylenetetrazole and Maximal Electroshock Seizures in Mice Basic and Clinical Neuroscience alcea aucheri pentylenetetrazole seizure threshold maximal electroshock seizure mice |
| title | Anticonvulsant Effect of Alcea aucheri on Pentylenetetrazole and Maximal Electroshock Seizures in Mice |
| title_full | Anticonvulsant Effect of Alcea aucheri on Pentylenetetrazole and Maximal Electroshock Seizures in Mice |
| title_fullStr | Anticonvulsant Effect of Alcea aucheri on Pentylenetetrazole and Maximal Electroshock Seizures in Mice |
| title_full_unstemmed | Anticonvulsant Effect of Alcea aucheri on Pentylenetetrazole and Maximal Electroshock Seizures in Mice |
| title_short | Anticonvulsant Effect of Alcea aucheri on Pentylenetetrazole and Maximal Electroshock Seizures in Mice |
| title_sort | anticonvulsant effect of alcea aucheri on pentylenetetrazole and maximal electroshock seizures in mice |
| topic | alcea aucheri pentylenetetrazole seizure threshold maximal electroshock seizure mice |
| url | http://bcn.iums.ac.ir/article-1-1605-en.html |
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