The Frequency of TIM-3-1541C>T Polymorphisms and its Association with Multiple Sclerosis
Background: Multiple sclerosis (MS) is an organ-specific autoimmune disease in central nervous system, which about 2.5 million people around the world are suffering from it. Various factors including environmental and genetic factors are involved in the incidence of the disease. T cell immunoglobuli...
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| Format: | Article |
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Isfahan University of Medical Sciences
2015-07-01
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| Series: | مجله دانشکده پزشکی اصفهان |
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| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/5028 |
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| author | Fariba Mazrouei Mazdak Ganjalikhani-Hakemi Rasoul Salehi Fereshteh Ale-Sahebfosoul Masoud Etemadifar Hamid Zarkesh-Esfahani |
| author_facet | Fariba Mazrouei Mazdak Ganjalikhani-Hakemi Rasoul Salehi Fereshteh Ale-Sahebfosoul Masoud Etemadifar Hamid Zarkesh-Esfahani |
| author_sort | Fariba Mazrouei |
| collection | DOAJ |
| description | Background: Multiple sclerosis (MS) is an organ-specific autoimmune disease in central nervous system, which about 2.5 million people around the world are suffering from it. Various factors including environmental and genetic factors are involved in the incidence of the disease. T cell immunoglobulin and mucin (TIM) gene is one of the genetic factors which recently demonstrated that has critical role in autoimmune diseases. In this study, the frequency and the association of TIM-3-1541C>T polymorphisms with multiple sclerosis disease in Isfahan, Iran, population were investigated.
Methods: Blood samples were collected from 140 patients with multiple sclerosis and 138 healthy controls. After DNA extraction, the TIM-3-1541C>T polymorphism was detected via polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) techniques. The results were analyzed with the SPSS16.0 software package.
Findings: The frequency of CT genotype in patients with multiple sclerosis was higher than the control group and there was a significant association between multiple sclerosis and TIM-3-1541C>T polymorphism in Isfahan population (P = 0.009, OR = 4.08).
Conclusion: T cell immunoglobulin and mucin gene family (TIM) is located on human chromosome 5q33.2 and play a critical role in regulating immune responses including allergy, transplant tolerance and autoimmunity. This study was done based on the results of previous studies about the association of TIM-3-1541C>T with rheumatoid arthritis, since the mechanisms of pathogenesis of this disease and multiple sclerosis are similar. Our results showed that TIM-3-1541C>T polymorphism is associated with multiple sclerosis in Isfahan population. |
| first_indexed | 2024-03-12T18:35:28Z |
| format | Article |
| id | doaj.art-f70b0114b53148f7938411f8765f9755 |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T18:35:28Z |
| publishDate | 2015-07-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-f70b0114b53148f7938411f8765f97552023-08-02T08:04:26ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2015-07-01333357397461879The Frequency of TIM-3-1541C>T Polymorphisms and its Association with Multiple SclerosisFariba Mazrouei0Mazdak Ganjalikhani-Hakemi1Rasoul Salehi2Fereshteh Ale-Sahebfosoul3Masoud Etemadifar4Hamid Zarkesh-Esfahani5MSc Student, Department of Immunology, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, IranAssistant Professor, Cellular and Molecular Immunology Research Center, Isfahan University of Medical Sciences, Isfahan, IranAssociate Professor, Department of Cellular and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranAssistant Professor, Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranProfessor, Department of Neurosciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranAssociate Professor, Department of Biology, School of Science, University of Isfahan, Isfahan, IranBackground: Multiple sclerosis (MS) is an organ-specific autoimmune disease in central nervous system, which about 2.5 million people around the world are suffering from it. Various factors including environmental and genetic factors are involved in the incidence of the disease. T cell immunoglobulin and mucin (TIM) gene is one of the genetic factors which recently demonstrated that has critical role in autoimmune diseases. In this study, the frequency and the association of TIM-3-1541C>T polymorphisms with multiple sclerosis disease in Isfahan, Iran, population were investigated. Methods: Blood samples were collected from 140 patients with multiple sclerosis and 138 healthy controls. After DNA extraction, the TIM-3-1541C>T polymorphism was detected via polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) techniques. The results were analyzed with the SPSS16.0 software package. Findings: The frequency of CT genotype in patients with multiple sclerosis was higher than the control group and there was a significant association between multiple sclerosis and TIM-3-1541C>T polymorphism in Isfahan population (P = 0.009, OR = 4.08). Conclusion: T cell immunoglobulin and mucin gene family (TIM) is located on human chromosome 5q33.2 and play a critical role in regulating immune responses including allergy, transplant tolerance and autoimmunity. This study was done based on the results of previous studies about the association of TIM-3-1541C>T with rheumatoid arthritis, since the mechanisms of pathogenesis of this disease and multiple sclerosis are similar. Our results showed that TIM-3-1541C>T polymorphism is associated with multiple sclerosis in Isfahan population.http://jims.mui.ac.ir/index.php/jims/article/view/5028Multiple sclerosisT cell immunoglobulin and mucin (TIM) genPolymorphism |
| spellingShingle | Fariba Mazrouei Mazdak Ganjalikhani-Hakemi Rasoul Salehi Fereshteh Ale-Sahebfosoul Masoud Etemadifar Hamid Zarkesh-Esfahani The Frequency of TIM-3-1541C>T Polymorphisms and its Association with Multiple Sclerosis مجله دانشکده پزشکی اصفهان Multiple sclerosis T cell immunoglobulin and mucin (TIM) gen Polymorphism |
| title | The Frequency of TIM-3-1541C>T Polymorphisms and its Association with Multiple Sclerosis |
| title_full | The Frequency of TIM-3-1541C>T Polymorphisms and its Association with Multiple Sclerosis |
| title_fullStr | The Frequency of TIM-3-1541C>T Polymorphisms and its Association with Multiple Sclerosis |
| title_full_unstemmed | The Frequency of TIM-3-1541C>T Polymorphisms and its Association with Multiple Sclerosis |
| title_short | The Frequency of TIM-3-1541C>T Polymorphisms and its Association with Multiple Sclerosis |
| title_sort | frequency of tim 3 1541c t polymorphisms and its association with multiple sclerosis |
| topic | Multiple sclerosis T cell immunoglobulin and mucin (TIM) gen Polymorphism |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/5028 |
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