NLRP3 inflammasome activation mediates terahertz exposure-induced skin aseptic inflammation in mice
Objective To investigate the biological effects of terahertz (THz) exposure on the skin injury of mice, and explore the mechanism of the induced skin aseptic inflammation. Methods The hair-removed skin in the back of C57BL/6J mice were exposed to 0.22 THz irradiation, at a peak power density of 25,...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
Editorial Office of Journal of Third Military Medical University
2020-12-01
|
| Series: | Di-san junyi daxue xuebao |
| Subjects: | |
| Online Access: | http://aammt.tmmu.edu.cn/Upload/rhtml/202008142.htm |
| _version_ | 1819282249141452800 |
|---|---|
| author | GAO Peng LU Yonghui MA Qinlong LI Min CHEN Chunhai HE Mindi YU Zhengping |
| author_facet | GAO Peng LU Yonghui MA Qinlong LI Min CHEN Chunhai HE Mindi YU Zhengping |
| author_sort | GAO Peng |
| collection | DOAJ |
| description | Objective To investigate the biological effects of terahertz (THz) exposure on the skin injury of mice, and explore the mechanism of the induced skin aseptic inflammation. Methods The hair-removed skin in the back of C57BL/6J mice were exposed to 0.22 THz irradiation, at a peak power density of 25, 80 and 160 mW/cm2, respectively and a duty factor of 1: 5, for 5 or 10 min. The release of inflammatory factors, oxidative stress and activation of NLR P3 inflammasome were detected after the exposure. Caspase-1 specific inhibitor Z-YVAD was employed to determine the role of the activation in the inflammation. Results Exposure to 160 mW/cm2 THz irradiation for 10 min induced oxidative stress, and the releases of PGE2, IL-1β and IL-6 in the skin (P < 0.05). And the 80 mW/cm2 and 160 mW/cm2 THz exposure activated the NLRP3 inflammasome in the skin, and the Caspase-1 inhibitor reduced the inflammatory factors induced by the THz irradiation (P < 0.05). Conclusion THz exposure induces oxidative stress and aseptic inflammation. Activation of NLRP3 inflammasome mediates the skin aseptic inflammation induced by THz irradiation, which might be associated with oxidative stress. |
| first_indexed | 2024-12-24T01:12:35Z |
| format | Article |
| id | doaj.art-f70b0cecfdec4a27ba93e5c374bb9981 |
| institution | Directory Open Access Journal |
| issn | 1000-5404 |
| language | zho |
| last_indexed | 2024-12-24T01:12:35Z |
| publishDate | 2020-12-01 |
| publisher | Editorial Office of Journal of Third Military Medical University |
| record_format | Article |
| series | Di-san junyi daxue xuebao |
| spelling | doaj.art-f70b0cecfdec4a27ba93e5c374bb99812022-12-21T17:22:52ZzhoEditorial Office of Journal of Third Military Medical UniversityDi-san junyi daxue xuebao1000-54042020-12-0142232290229510.16016/j.1000-5404.202008142NLRP3 inflammasome activation mediates terahertz exposure-induced skin aseptic inflammation in miceGAO Peng0LU Yonghui1MA Qinlong2LI Min3CHEN Chunhai4 HE Mindi5YU Zhengping6Department of Occupational Health, Key Laboratory of Electromagnetic Radiation Protection of Ministry of Education, Faculty of Military Preventive Medicine, Army Medical University (Third Military Medical University), Chongqing, 400038, China Department of Occupational Health, Key Laboratory of Electromagnetic Radiation Protection of Ministry of Education, Faculty of Military Preventive Medicine, Army Medical University (Third Military Medical University), Chongqing, 400038, China Department of Occupational Health, Key Laboratory of Electromagnetic Radiation Protection of Ministry of Education, Faculty of Military Preventive Medicine, Army Medical University (Third Military Medical University), Chongqing, 400038, China Department of Occupational Health, Key Laboratory of Electromagnetic Radiation Protection of Ministry of Education, Faculty of Military Preventive Medicine, Army Medical University (Third Military Medical University), Chongqing, 400038, China Department of Occupational Health, Key Laboratory of Electromagnetic Radiation Protection of Ministry of Education, Faculty of Military Preventive Medicine, Army Medical University (Third Military Medical University), Chongqing, 400038, China Department of Occupational Health, Key Laboratory of Electromagnetic Radiation Protection of Ministry of Education, Faculty of Military Preventive Medicine, Army Medical University (Third Military Medical University), Chongqing, 400038, China Department of Occupational Health, Key Laboratory of Electromagnetic Radiation Protection of Ministry of Education, Faculty of Military Preventive Medicine, Army Medical University (Third Military Medical University), Chongqing, 400038, China Objective To investigate the biological effects of terahertz (THz) exposure on the skin injury of mice, and explore the mechanism of the induced skin aseptic inflammation. Methods The hair-removed skin in the back of C57BL/6J mice were exposed to 0.22 THz irradiation, at a peak power density of 25, 80 and 160 mW/cm2, respectively and a duty factor of 1: 5, for 5 or 10 min. The release of inflammatory factors, oxidative stress and activation of NLR P3 inflammasome were detected after the exposure. Caspase-1 specific inhibitor Z-YVAD was employed to determine the role of the activation in the inflammation. Results Exposure to 160 mW/cm2 THz irradiation for 10 min induced oxidative stress, and the releases of PGE2, IL-1β and IL-6 in the skin (P < 0.05). And the 80 mW/cm2 and 160 mW/cm2 THz exposure activated the NLRP3 inflammasome in the skin, and the Caspase-1 inhibitor reduced the inflammatory factors induced by the THz irradiation (P < 0.05). Conclusion THz exposure induces oxidative stress and aseptic inflammation. Activation of NLRP3 inflammasome mediates the skin aseptic inflammation induced by THz irradiation, which might be associated with oxidative stress.http://aammt.tmmu.edu.cn/Upload/rhtml/202008142.htmterahertzskininflammationnlrp3 inflammasomeoxidative stress |
| spellingShingle | GAO Peng LU Yonghui MA Qinlong LI Min CHEN Chunhai HE Mindi YU Zhengping NLRP3 inflammasome activation mediates terahertz exposure-induced skin aseptic inflammation in mice Di-san junyi daxue xuebao terahertz skin inflammation nlrp3 inflammasome oxidative stress |
| title | NLRP3 inflammasome activation mediates terahertz exposure-induced skin aseptic inflammation in mice |
| title_full | NLRP3 inflammasome activation mediates terahertz exposure-induced skin aseptic inflammation in mice |
| title_fullStr | NLRP3 inflammasome activation mediates terahertz exposure-induced skin aseptic inflammation in mice |
| title_full_unstemmed | NLRP3 inflammasome activation mediates terahertz exposure-induced skin aseptic inflammation in mice |
| title_short | NLRP3 inflammasome activation mediates terahertz exposure-induced skin aseptic inflammation in mice |
| title_sort | nlrp3 inflammasome activation mediates terahertz exposure induced skin aseptic inflammation in mice |
| topic | terahertz skin inflammation nlrp3 inflammasome oxidative stress |
| url | http://aammt.tmmu.edu.cn/Upload/rhtml/202008142.htm |
| work_keys_str_mv | AT gaopeng nlrp3inflammasomeactivationmediatesterahertzexposureinducedskinasepticinflammationinmice AT luyonghui nlrp3inflammasomeactivationmediatesterahertzexposureinducedskinasepticinflammationinmice AT maqinlong nlrp3inflammasomeactivationmediatesterahertzexposureinducedskinasepticinflammationinmice AT limin nlrp3inflammasomeactivationmediatesterahertzexposureinducedskinasepticinflammationinmice AT chenchunhai nlrp3inflammasomeactivationmediatesterahertzexposureinducedskinasepticinflammationinmice AT hemindi nlrp3inflammasomeactivationmediatesterahertzexposureinducedskinasepticinflammationinmice AT yuzhengping nlrp3inflammasomeactivationmediatesterahertzexposureinducedskinasepticinflammationinmice |