Membrane-Suspended Nanopores in Microchip Arrays for Stochastic Transport Recording and Sensing
The transport of nutrients, xenobiotics, and signaling molecules across biological membranes is essential for life. As gatekeepers of cells, membrane proteins and nanopores are key targets in pharmaceutical research and industry. Multiple techniques help in elucidating, utilizing, or mimicking the f...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-06-01
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| Series: | Frontiers in Nanotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnano.2021.703673/full |
| _version_ | 1818670536075509760 |
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| author | Tim Diederichs Robert Tampé |
| author_facet | Tim Diederichs Robert Tampé |
| author_sort | Tim Diederichs |
| collection | DOAJ |
| description | The transport of nutrients, xenobiotics, and signaling molecules across biological membranes is essential for life. As gatekeepers of cells, membrane proteins and nanopores are key targets in pharmaceutical research and industry. Multiple techniques help in elucidating, utilizing, or mimicking the function of biological membrane-embedded nanodevices. In particular, the use of DNA origami to construct simple nanopores based on the predictable folding of nucleotides provides a promising direction for innovative sensing and sequencing approaches. Knowledge of translocation characteristics is crucial to link structural design with function. Here, we summarize recent developments and compare features of membrane-embedded nanopores with solid-state analogues. We also describe how their translocation properties are characterized by microchip systems. The recently developed silicon chips, comprising solid-state nanopores of 80 nm connecting femtoliter cavities in combination with vesicle spreading and formation of nanopore-suspended membranes, will pave the way to characterize translocation properties of nanopores and membrane proteins in high-throughput and at single-transporter resolution. |
| first_indexed | 2024-12-17T07:09:40Z |
| format | Article |
| id | doaj.art-f70b65d35b7645b1941ae30590712a02 |
| institution | Directory Open Access Journal |
| issn | 2673-3013 |
| language | English |
| last_indexed | 2024-12-17T07:09:40Z |
| publishDate | 2021-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nanotechnology |
| spelling | doaj.art-f70b65d35b7645b1941ae30590712a022022-12-21T21:59:05ZengFrontiers Media S.A.Frontiers in Nanotechnology2673-30132021-06-01310.3389/fnano.2021.703673703673Membrane-Suspended Nanopores in Microchip Arrays for Stochastic Transport Recording and SensingTim DiederichsRobert TampéThe transport of nutrients, xenobiotics, and signaling molecules across biological membranes is essential for life. As gatekeepers of cells, membrane proteins and nanopores are key targets in pharmaceutical research and industry. Multiple techniques help in elucidating, utilizing, or mimicking the function of biological membrane-embedded nanodevices. In particular, the use of DNA origami to construct simple nanopores based on the predictable folding of nucleotides provides a promising direction for innovative sensing and sequencing approaches. Knowledge of translocation characteristics is crucial to link structural design with function. Here, we summarize recent developments and compare features of membrane-embedded nanopores with solid-state analogues. We also describe how their translocation properties are characterized by microchip systems. The recently developed silicon chips, comprising solid-state nanopores of 80 nm connecting femtoliter cavities in combination with vesicle spreading and formation of nanopore-suspended membranes, will pave the way to characterize translocation properties of nanopores and membrane proteins in high-throughput and at single-transporter resolution.https://www.frontiersin.org/articles/10.3389/fnano.2021.703673/fullDNA nanoporessolid-state nanoporesmembrane proteinsmembrane transportsilicon chipsbiosenors |
| spellingShingle | Tim Diederichs Robert Tampé Membrane-Suspended Nanopores in Microchip Arrays for Stochastic Transport Recording and Sensing Frontiers in Nanotechnology DNA nanopores solid-state nanopores membrane proteins membrane transport silicon chips biosenors |
| title | Membrane-Suspended Nanopores in Microchip Arrays for Stochastic Transport Recording and Sensing |
| title_full | Membrane-Suspended Nanopores in Microchip Arrays for Stochastic Transport Recording and Sensing |
| title_fullStr | Membrane-Suspended Nanopores in Microchip Arrays for Stochastic Transport Recording and Sensing |
| title_full_unstemmed | Membrane-Suspended Nanopores in Microchip Arrays for Stochastic Transport Recording and Sensing |
| title_short | Membrane-Suspended Nanopores in Microchip Arrays for Stochastic Transport Recording and Sensing |
| title_sort | membrane suspended nanopores in microchip arrays for stochastic transport recording and sensing |
| topic | DNA nanopores solid-state nanopores membrane proteins membrane transport silicon chips biosenors |
| url | https://www.frontiersin.org/articles/10.3389/fnano.2021.703673/full |
| work_keys_str_mv | AT timdiederichs membranesuspendednanoporesinmicrochiparraysforstochastictransportrecordingandsensing AT roberttampe membranesuspendednanoporesinmicrochiparraysforstochastictransportrecordingandsensing |