Collagen cross-linking as monotherapy in experimentally induced corneal abscess in rabbits
Abstract Background and purpose Collagen cross-linking (CXL) has evolved as an essential therapeutic approach for corneal infections, allowing for rapidly eliminating the infecting microorganism while reducing inflammation. This study aims to evaluate the efficacy of CXL as a monotherapy for managin...
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| Format: | Article |
| Language: | English |
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BMC
2023-06-01
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| Series: | BMC Ophthalmology |
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| Online Access: | https://doi.org/10.1186/s12886-023-03007-y |
| _version_ | 1797801544674967552 |
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| author | Zeinab A. Saad Hazem Elnashar Sahar Negm Hala A. Elsayed Mohamed Gaber Abdallah Tamer M. M. Abuamara Wagih M. Abd-Elhay Hanan M. Elghonemy |
| author_facet | Zeinab A. Saad Hazem Elnashar Sahar Negm Hala A. Elsayed Mohamed Gaber Abdallah Tamer M. M. Abuamara Wagih M. Abd-Elhay Hanan M. Elghonemy |
| author_sort | Zeinab A. Saad |
| collection | DOAJ |
| description | Abstract Background and purpose Collagen cross-linking (CXL) has evolved as an essential therapeutic approach for corneal infections, allowing for rapidly eliminating the infecting microorganism while reducing inflammation. This study aims to evaluate the efficacy of CXL as a monotherapy for managing infectious keratitis caused by Fusarium solani and Pseudomonas aeruginosa. Materials and methods Forty-eight white New Zealand rabbits weighing approximately 1.5–2 KG were included. The cornea of one eye of each rabbit was inoculated with either Fusarium solani or Pseudomonas aeruginosa. Group A served as a control and was subdivided into two subgroups, A1 and A2; each subgroup consisted of 8 eyes and was injected with either Fusarium solani or Pseudomonas aeruginosa, respectively. Group B (16 eyes) was inoculated with Fusarium solani, while group C (16 eyes) were inoculated with Pseudomonas aeruginosa. All animals in Group B and C received CXL treatment one week after inoculation of the organisms and after corneal abscess formation was confirmed. At the same time, animals in Group A were left untreated. Results There was a statistically significant reduction in the number of colony-forming units (CFU) in Group B following CXL. No growth existed in any samples at the end of the 4th week. There was a statistically significant difference in the number of CFU between group B and the control group (p < 0.001). In group C, there was a statistically significant reduction in the CFU at the end of the first week after CXL. However, there was regrowth in all samples afterward. All 16 models in Group C showed uncountable and extensive growth during the subsequent follow-ups. There was no statistically significant difference between the number of CFU in Group C and the control group. Histopathology showed lesser corneal melting in CXL-treated Pseudomonas aeruginosa. Conclusions Collagen cross-linking is promising monotherapy and alternative treatment in managing infective keratitis caused by Fusarium solani but is less effective in Pseudomonas aeruginosa as monotherapy. |
| first_indexed | 2024-03-13T04:52:06Z |
| format | Article |
| id | doaj.art-f7121d9e3ffb45e49c4ee38d678ad77f |
| institution | Directory Open Access Journal |
| issn | 1471-2415 |
| language | English |
| last_indexed | 2024-03-13T04:52:06Z |
| publishDate | 2023-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Ophthalmology |
| spelling | doaj.art-f7121d9e3ffb45e49c4ee38d678ad77f2023-06-18T11:09:48ZengBMCBMC Ophthalmology1471-24152023-06-012311810.1186/s12886-023-03007-yCollagen cross-linking as monotherapy in experimentally induced corneal abscess in rabbitsZeinab A. Saad0Hazem Elnashar1Sahar Negm2Hala A. Elsayed3Mohamed Gaber Abdallah4Tamer M. M. Abuamara5Wagih M. Abd-Elhay6Hanan M. Elghonemy7Memorial Institute of Ophthalmic ResearchMemorial Institute of Ophthalmic ResearchResearch Institute of OphthalmologyMemorial Institute of Ophthalmic ResearchFaculty of Medicine, Department of Medical Biochemistry, Al-Azhar UniversityFaculty of Medicine, Histology Department, Al-Azhar UniversityFaculty of Medicine, Histology Department, Al-Azhar UniversityMemorial Institute of Ophthalmic ResearchAbstract Background and purpose Collagen cross-linking (CXL) has evolved as an essential therapeutic approach for corneal infections, allowing for rapidly eliminating the infecting microorganism while reducing inflammation. This study aims to evaluate the efficacy of CXL as a monotherapy for managing infectious keratitis caused by Fusarium solani and Pseudomonas aeruginosa. Materials and methods Forty-eight white New Zealand rabbits weighing approximately 1.5–2 KG were included. The cornea of one eye of each rabbit was inoculated with either Fusarium solani or Pseudomonas aeruginosa. Group A served as a control and was subdivided into two subgroups, A1 and A2; each subgroup consisted of 8 eyes and was injected with either Fusarium solani or Pseudomonas aeruginosa, respectively. Group B (16 eyes) was inoculated with Fusarium solani, while group C (16 eyes) were inoculated with Pseudomonas aeruginosa. All animals in Group B and C received CXL treatment one week after inoculation of the organisms and after corneal abscess formation was confirmed. At the same time, animals in Group A were left untreated. Results There was a statistically significant reduction in the number of colony-forming units (CFU) in Group B following CXL. No growth existed in any samples at the end of the 4th week. There was a statistically significant difference in the number of CFU between group B and the control group (p < 0.001). In group C, there was a statistically significant reduction in the CFU at the end of the first week after CXL. However, there was regrowth in all samples afterward. All 16 models in Group C showed uncountable and extensive growth during the subsequent follow-ups. There was no statistically significant difference between the number of CFU in Group C and the control group. Histopathology showed lesser corneal melting in CXL-treated Pseudomonas aeruginosa. Conclusions Collagen cross-linking is promising monotherapy and alternative treatment in managing infective keratitis caused by Fusarium solani but is less effective in Pseudomonas aeruginosa as monotherapy.https://doi.org/10.1186/s12886-023-03007-yCollagen cross-linkingMicrobial keratitisFusarium solaniPseudomonas aeruginosa |
| spellingShingle | Zeinab A. Saad Hazem Elnashar Sahar Negm Hala A. Elsayed Mohamed Gaber Abdallah Tamer M. M. Abuamara Wagih M. Abd-Elhay Hanan M. Elghonemy Collagen cross-linking as monotherapy in experimentally induced corneal abscess in rabbits BMC Ophthalmology Collagen cross-linking Microbial keratitis Fusarium solani Pseudomonas aeruginosa |
| title | Collagen cross-linking as monotherapy in experimentally induced corneal abscess in rabbits |
| title_full | Collagen cross-linking as monotherapy in experimentally induced corneal abscess in rabbits |
| title_fullStr | Collagen cross-linking as monotherapy in experimentally induced corneal abscess in rabbits |
| title_full_unstemmed | Collagen cross-linking as monotherapy in experimentally induced corneal abscess in rabbits |
| title_short | Collagen cross-linking as monotherapy in experimentally induced corneal abscess in rabbits |
| title_sort | collagen cross linking as monotherapy in experimentally induced corneal abscess in rabbits |
| topic | Collagen cross-linking Microbial keratitis Fusarium solani Pseudomonas aeruginosa |
| url | https://doi.org/10.1186/s12886-023-03007-y |
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