Phenotypic Characterization and Differential Gene Expression Analysis Reveal That Dwarf Mutant <i>dwf</i> Dwarfism Is Associated with Gibberellin in Eggplant
Dwarfism is a desirable trait in eggplant breeding, as it confers higher lodging resistance and allows simplified management and harvest. However, a few dwarf mutants have been reported, and the molecular mechanism underlying dwarfism in eggplant is completely unknown. Here, we report a dwarf mutant...
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MDPI AG
2021-05-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/7/5/114 |
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| author | Yang Lu Shuangxia Luo Qiang Li Na Li Wenchao Du Ping Yu Xing Wang Weiwei Zhang Shuxin Xuan Xuan Zhou Jiajia Shen Jianjun Zhao Yanhua Wang Xueping Chen Shuxing Shen |
| author_facet | Yang Lu Shuangxia Luo Qiang Li Na Li Wenchao Du Ping Yu Xing Wang Weiwei Zhang Shuxin Xuan Xuan Zhou Jiajia Shen Jianjun Zhao Yanhua Wang Xueping Chen Shuxing Shen |
| author_sort | Yang Lu |
| collection | DOAJ |
| description | Dwarfism is a desirable trait in eggplant breeding, as it confers higher lodging resistance and allows simplified management and harvest. However, a few dwarf mutants have been reported, and the molecular mechanism underlying dwarfism in eggplant is completely unknown. Here, we report a dwarf mutant (<i>dwf</i>) isolated from an ethyl methyl sulfonate (EMS)-induced mutant library. The hypocotyl length, plant height, and length of internode cells of <i>dwf</i> were significantly decreased compared to those of the wild-type parent ‘14-345’ (WT). Differential gene expression analysis revealed that GA-related genes, including <i>GA2ox</i> and <i>DELLA,</i> were up-regulated whereas the gibberellin (GA<sub>3</sub>) content decreased in <i>dwf</i>. Moreover, exogenous GA<sub>3</sub> treatment significantly increased the relative growth rate of <i>dwf</i> compared to WT, further indicating the important roles of GA in regulating the dwarf phenotype of <i>dwf</i>. Collectively, our findings shed light on GA-mediated dwarfism in <i>dwf</i> plants and offer a good germplasm that could be used for eggplant dwarfism breeding in the future. |
| first_indexed | 2024-03-10T11:22:31Z |
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| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-10T11:22:31Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
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| series | Horticulturae |
| spelling | doaj.art-f714cf1226824e24b92ba42ff1523c212023-11-21T19:58:45ZengMDPI AGHorticulturae2311-75242021-05-017511410.3390/horticulturae7050114Phenotypic Characterization and Differential Gene Expression Analysis Reveal That Dwarf Mutant <i>dwf</i> Dwarfism Is Associated with Gibberellin in EggplantYang Lu0Shuangxia Luo1Qiang Li2Na Li3Wenchao Du4Ping Yu5Xing Wang6Weiwei Zhang7Shuxin Xuan8Xuan Zhou9Jiajia Shen10Jianjun Zhao11Yanhua Wang12Xueping Chen13Shuxing Shen14Key Laboratory for Vegetable Germplasm Enhancement and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Hebei Agricultural University, Baoding 071000, ChinaKey Laboratory for Vegetable Germplasm Enhancement and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Hebei Agricultural University, Baoding 071000, ChinaKey Laboratory for Vegetable Germplasm Enhancement and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Hebei Agricultural University, Baoding 071000, ChinaKey Laboratory for Vegetable Germplasm Enhancement and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Hebei Agricultural University, Baoding 071000, ChinaKey Laboratory for Vegetable Germplasm Enhancement and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Hebei Agricultural University, Baoding 071000, ChinaKey Laboratory for Vegetable Germplasm Enhancement and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Hebei Agricultural University, Baoding 071000, ChinaKey Laboratory for Vegetable Germplasm Enhancement and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Hebei Agricultural University, Baoding 071000, ChinaCollege of Life Sciences, Hebei Agricultural University, Baoding 071000, ChinaKey Laboratory for Vegetable Germplasm Enhancement and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Hebei Agricultural University, Baoding 071000, ChinaKey Laboratory for Vegetable Germplasm Enhancement and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Hebei Agricultural University, Baoding 071000, ChinaKey Laboratory for Vegetable Germplasm Enhancement and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Hebei Agricultural University, Baoding 071000, ChinaKey Laboratory for Vegetable Germplasm Enhancement and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Hebei Agricultural University, Baoding 071000, ChinaKey Laboratory for Vegetable Germplasm Enhancement and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Hebei Agricultural University, Baoding 071000, ChinaKey Laboratory for Vegetable Germplasm Enhancement and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Hebei Agricultural University, Baoding 071000, ChinaKey Laboratory for Vegetable Germplasm Enhancement and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Hebei Agricultural University, Baoding 071000, ChinaDwarfism is a desirable trait in eggplant breeding, as it confers higher lodging resistance and allows simplified management and harvest. However, a few dwarf mutants have been reported, and the molecular mechanism underlying dwarfism in eggplant is completely unknown. Here, we report a dwarf mutant (<i>dwf</i>) isolated from an ethyl methyl sulfonate (EMS)-induced mutant library. The hypocotyl length, plant height, and length of internode cells of <i>dwf</i> were significantly decreased compared to those of the wild-type parent ‘14-345’ (WT). Differential gene expression analysis revealed that GA-related genes, including <i>GA2ox</i> and <i>DELLA,</i> were up-regulated whereas the gibberellin (GA<sub>3</sub>) content decreased in <i>dwf</i>. Moreover, exogenous GA<sub>3</sub> treatment significantly increased the relative growth rate of <i>dwf</i> compared to WT, further indicating the important roles of GA in regulating the dwarf phenotype of <i>dwf</i>. Collectively, our findings shed light on GA-mediated dwarfism in <i>dwf</i> plants and offer a good germplasm that could be used for eggplant dwarfism breeding in the future.https://www.mdpi.com/2311-7524/7/5/114eggplantdwarfismplant heightgibberellindifferentially expressed gene |
| spellingShingle | Yang Lu Shuangxia Luo Qiang Li Na Li Wenchao Du Ping Yu Xing Wang Weiwei Zhang Shuxin Xuan Xuan Zhou Jiajia Shen Jianjun Zhao Yanhua Wang Xueping Chen Shuxing Shen Phenotypic Characterization and Differential Gene Expression Analysis Reveal That Dwarf Mutant <i>dwf</i> Dwarfism Is Associated with Gibberellin in Eggplant Horticulturae eggplant dwarfism plant height gibberellin differentially expressed gene |
| title | Phenotypic Characterization and Differential Gene Expression Analysis Reveal That Dwarf Mutant <i>dwf</i> Dwarfism Is Associated with Gibberellin in Eggplant |
| title_full | Phenotypic Characterization and Differential Gene Expression Analysis Reveal That Dwarf Mutant <i>dwf</i> Dwarfism Is Associated with Gibberellin in Eggplant |
| title_fullStr | Phenotypic Characterization and Differential Gene Expression Analysis Reveal That Dwarf Mutant <i>dwf</i> Dwarfism Is Associated with Gibberellin in Eggplant |
| title_full_unstemmed | Phenotypic Characterization and Differential Gene Expression Analysis Reveal That Dwarf Mutant <i>dwf</i> Dwarfism Is Associated with Gibberellin in Eggplant |
| title_short | Phenotypic Characterization and Differential Gene Expression Analysis Reveal That Dwarf Mutant <i>dwf</i> Dwarfism Is Associated with Gibberellin in Eggplant |
| title_sort | phenotypic characterization and differential gene expression analysis reveal that dwarf mutant i dwf i dwarfism is associated with gibberellin in eggplant |
| topic | eggplant dwarfism plant height gibberellin differentially expressed gene |
| url | https://www.mdpi.com/2311-7524/7/5/114 |
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