Histone Acetyltransferase SlGCN5 Regulates Shoot Meristem and Flower Development in Solanum lycopersicum
The histone acetyltransferase (HAT) general control non-repressed protein 5 (GCN5) plays important roles in plant development via epigenetic regulation of its target genes. However, the role of GCN5 in tomato, especially in the regulation of tomato shoot meristem and flower development, has not been...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-01-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.805879/full |
| _version_ | 1818971981492518912 |
|---|---|
| author | Amangul Hawar Shiqi Xiong Zhen Yang Bo Sun |
| author_facet | Amangul Hawar Shiqi Xiong Zhen Yang Bo Sun |
| author_sort | Amangul Hawar |
| collection | DOAJ |
| description | The histone acetyltransferase (HAT) general control non-repressed protein 5 (GCN5) plays important roles in plant development via epigenetic regulation of its target genes. However, the role of GCN5 in tomato, especially in the regulation of tomato shoot meristem and flower development, has not been well-understood. In this study, we found that silencing of Solanum lycopersicum GCN5 (SlGCN5, Solyc10g045400.1.1) by virus-induced gene silencing (VIGS) and RNA interference (RNAi) resulted in the loss of shoot apical dominance, reduced shoot apical meristem (SAM) size, and dwarf and bushy plant phenotype. Besides, we occasionally observed extra carpelloid stamens and carpels fused with stamens at the late stages of flower development. Through gene expression analysis, we noticed that SlGCN5 could enhance SlWUS transcript levels in both SAM and floral meristem (FM). Similar to the known function of GCN5 in Arabidopsis, we demonstrated that SIGCN5 may form a HAT unit with S. lycopersicum alteration/deficiency in activation 2a (SlADA2a) and SlADA2b proteins in tomato. Therefore, our results provide insights in the SlGCN5-mediated regulation of SAM maintenance and floral development in tomato. |
| first_indexed | 2024-12-20T15:01:00Z |
| format | Article |
| id | doaj.art-f714dccb0b784446a993a97228b670aa |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-20T15:01:00Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-f714dccb0b784446a993a97228b670aa2022-12-21T19:36:40ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-01-011210.3389/fpls.2021.805879805879Histone Acetyltransferase SlGCN5 Regulates Shoot Meristem and Flower Development in Solanum lycopersicumAmangul HawarShiqi XiongZhen YangBo SunThe histone acetyltransferase (HAT) general control non-repressed protein 5 (GCN5) plays important roles in plant development via epigenetic regulation of its target genes. However, the role of GCN5 in tomato, especially in the regulation of tomato shoot meristem and flower development, has not been well-understood. In this study, we found that silencing of Solanum lycopersicum GCN5 (SlGCN5, Solyc10g045400.1.1) by virus-induced gene silencing (VIGS) and RNA interference (RNAi) resulted in the loss of shoot apical dominance, reduced shoot apical meristem (SAM) size, and dwarf and bushy plant phenotype. Besides, we occasionally observed extra carpelloid stamens and carpels fused with stamens at the late stages of flower development. Through gene expression analysis, we noticed that SlGCN5 could enhance SlWUS transcript levels in both SAM and floral meristem (FM). Similar to the known function of GCN5 in Arabidopsis, we demonstrated that SIGCN5 may form a HAT unit with S. lycopersicum alteration/deficiency in activation 2a (SlADA2a) and SlADA2b proteins in tomato. Therefore, our results provide insights in the SlGCN5-mediated regulation of SAM maintenance and floral development in tomato.https://www.frontiersin.org/articles/10.3389/fpls.2021.805879/fulltomatoSAMSlGCN5SlWUSSlADA2 |
| spellingShingle | Amangul Hawar Shiqi Xiong Zhen Yang Bo Sun Histone Acetyltransferase SlGCN5 Regulates Shoot Meristem and Flower Development in Solanum lycopersicum Frontiers in Plant Science tomato SAM SlGCN5 SlWUS SlADA2 |
| title | Histone Acetyltransferase SlGCN5 Regulates Shoot Meristem and Flower Development in Solanum lycopersicum |
| title_full | Histone Acetyltransferase SlGCN5 Regulates Shoot Meristem and Flower Development in Solanum lycopersicum |
| title_fullStr | Histone Acetyltransferase SlGCN5 Regulates Shoot Meristem and Flower Development in Solanum lycopersicum |
| title_full_unstemmed | Histone Acetyltransferase SlGCN5 Regulates Shoot Meristem and Flower Development in Solanum lycopersicum |
| title_short | Histone Acetyltransferase SlGCN5 Regulates Shoot Meristem and Flower Development in Solanum lycopersicum |
| title_sort | histone acetyltransferase slgcn5 regulates shoot meristem and flower development in solanum lycopersicum |
| topic | tomato SAM SlGCN5 SlWUS SlADA2 |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.805879/full |
| work_keys_str_mv | AT amangulhawar histoneacetyltransferaseslgcn5regulatesshootmeristemandflowerdevelopmentinsolanumlycopersicum AT shiqixiong histoneacetyltransferaseslgcn5regulatesshootmeristemandflowerdevelopmentinsolanumlycopersicum AT zhenyang histoneacetyltransferaseslgcn5regulatesshootmeristemandflowerdevelopmentinsolanumlycopersicum AT bosun histoneacetyltransferaseslgcn5regulatesshootmeristemandflowerdevelopmentinsolanumlycopersicum |