Changes in the gene expression profile during spontaneous migraine attacks
Abstract Migraine attacks are delimited, allowing investigation of changes during and outside attack. Gene expression fluctuates according to environmental and endogenous events and therefore, we hypothesized that changes in RNA expression during and outside a spontaneous migraine attack exist which...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-87503-5 |
| _version_ | 1818844040204910592 |
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| author | Lisette J. A. Kogelman Katrine Falkenberg Alfonso Buil Pau Erola Julie Courraud Susan Svane Laursen Tom Michoel Jes Olesen Thomas F. Hansen |
| author_facet | Lisette J. A. Kogelman Katrine Falkenberg Alfonso Buil Pau Erola Julie Courraud Susan Svane Laursen Tom Michoel Jes Olesen Thomas F. Hansen |
| author_sort | Lisette J. A. Kogelman |
| collection | DOAJ |
| description | Abstract Migraine attacks are delimited, allowing investigation of changes during and outside attack. Gene expression fluctuates according to environmental and endogenous events and therefore, we hypothesized that changes in RNA expression during and outside a spontaneous migraine attack exist which are specific to migraine. Twenty-seven migraine patients were assessed during a spontaneous migraine attack, including headache characteristics and treatment effect. Blood samples were taken during attack, two hours after treatment, on a headache-free day and after a cold pressor test. RNA-Sequencing, genotyping, and steroid profiling were performed. RNA-Sequences were analyzed at gene level (differential expression analysis) and at network level, and genomic and transcriptomic data were integrated. We found 29 differentially expressed genes between ‘attack’ and ‘after treatment’, after subtracting non-migraine specific genes, that were functioning in fatty acid oxidation, signaling pathways and immune-related pathways. Network analysis revealed mechanisms affected by changes in gene interactions, e.g. ‘ion transmembrane transport’. Integration of genomic and transcriptomic data revealed pathways related to sumatriptan treatment, i.e. ‘5HT1 type receptor mediated signaling pathway’. In conclusion, we uniquely investigated intra-individual changes in gene expression during a migraine attack. We revealed both genes and pathways potentially involved in the pathophysiology of migraine and/or migraine treatment. |
| first_indexed | 2024-12-19T05:07:26Z |
| format | Article |
| id | doaj.art-f715f3d6980b4cd2a3675cf887e67e77 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T05:07:26Z |
| publishDate | 2021-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-f715f3d6980b4cd2a3675cf887e67e772022-12-21T20:34:54ZengNature PortfolioScientific Reports2045-23222021-04-0111111010.1038/s41598-021-87503-5Changes in the gene expression profile during spontaneous migraine attacksLisette J. A. Kogelman0Katrine Falkenberg1Alfonso Buil2Pau Erola3Julie Courraud4Susan Svane Laursen5Tom Michoel6Jes Olesen7Thomas F. Hansen8Danish Headache Center, Department of Neurology, Rigshospitalet GlostrupDanish Headache Center, Department of Neurology, Rigshospitalet GlostrupInstitute for Biological Psychiatry, Mental Health Center Sct. HansMRC Integrative Epidemiology Unit, University of BristolDepartment of Clinical Biochemistry and Immunology, Statens Serum Institute CopenhagenDepartment of Clinical Biochemistry and Immunology, Statens Serum Institute CopenhagenComputational Biology Unit, Department of Informatics, University of BergenDanish Headache Center, Department of Neurology, Rigshospitalet GlostrupDanish Headache Center, Department of Neurology, Rigshospitalet GlostrupAbstract Migraine attacks are delimited, allowing investigation of changes during and outside attack. Gene expression fluctuates according to environmental and endogenous events and therefore, we hypothesized that changes in RNA expression during and outside a spontaneous migraine attack exist which are specific to migraine. Twenty-seven migraine patients were assessed during a spontaneous migraine attack, including headache characteristics and treatment effect. Blood samples were taken during attack, two hours after treatment, on a headache-free day and after a cold pressor test. RNA-Sequencing, genotyping, and steroid profiling were performed. RNA-Sequences were analyzed at gene level (differential expression analysis) and at network level, and genomic and transcriptomic data were integrated. We found 29 differentially expressed genes between ‘attack’ and ‘after treatment’, after subtracting non-migraine specific genes, that were functioning in fatty acid oxidation, signaling pathways and immune-related pathways. Network analysis revealed mechanisms affected by changes in gene interactions, e.g. ‘ion transmembrane transport’. Integration of genomic and transcriptomic data revealed pathways related to sumatriptan treatment, i.e. ‘5HT1 type receptor mediated signaling pathway’. In conclusion, we uniquely investigated intra-individual changes in gene expression during a migraine attack. We revealed both genes and pathways potentially involved in the pathophysiology of migraine and/or migraine treatment.https://doi.org/10.1038/s41598-021-87503-5 |
| spellingShingle | Lisette J. A. Kogelman Katrine Falkenberg Alfonso Buil Pau Erola Julie Courraud Susan Svane Laursen Tom Michoel Jes Olesen Thomas F. Hansen Changes in the gene expression profile during spontaneous migraine attacks Scientific Reports |
| title | Changes in the gene expression profile during spontaneous migraine attacks |
| title_full | Changes in the gene expression profile during spontaneous migraine attacks |
| title_fullStr | Changes in the gene expression profile during spontaneous migraine attacks |
| title_full_unstemmed | Changes in the gene expression profile during spontaneous migraine attacks |
| title_short | Changes in the gene expression profile during spontaneous migraine attacks |
| title_sort | changes in the gene expression profile during spontaneous migraine attacks |
| url | https://doi.org/10.1038/s41598-021-87503-5 |
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