Changes in Microbiota Profile in the Proximal Remnant Intestine in Infants Undergoing Surgery Requiring Enterostomy
Early-life gut dysbiosis has been associated with an increased risk of inflammatory, metabolic, and immune diseases later in life. Data on gut microbiota changes in infants undergoing intestinal surgery requiring enterostomy are scarce. This prospective cohort study examined the enterostomy effluent...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/10/2482 |
| _version_ | 1827720354707013632 |
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| author | Inês Barreiros-Mota João R. Araújo Cláudia Marques Laura Sousa Juliana Morais Inês Castela Ana Faria Maria Teresa Neto Gonçalo Cordeiro-Ferreira Daniel Virella Ana Pita Luís Pereira-da-Silva Conceição Calhau |
| author_facet | Inês Barreiros-Mota João R. Araújo Cláudia Marques Laura Sousa Juliana Morais Inês Castela Ana Faria Maria Teresa Neto Gonçalo Cordeiro-Ferreira Daniel Virella Ana Pita Luís Pereira-da-Silva Conceição Calhau |
| author_sort | Inês Barreiros-Mota |
| collection | DOAJ |
| description | Early-life gut dysbiosis has been associated with an increased risk of inflammatory, metabolic, and immune diseases later in life. Data on gut microbiota changes in infants undergoing intestinal surgery requiring enterostomy are scarce. This prospective cohort study examined the enterostomy effluent of 29 infants who underwent intestinal surgery due to congenital malformations of the gastrointestinal tract, necrotizing enterocolitis, or spontaneous intestinal perforation. Initial effluent samples were collected immediately after surgery and final effluent samples were collected three weeks later. Gut microbiota composition was analysed using real-time PCR and 16S rRNA gene sequencing. Three weeks after surgery, an increase in total bacteria number (+21%, <i>p</i> = 0.026), a decrease in <i>Staphylococcus</i> (−21%, <i>p</i> = 0.002) and <i>Candida</i> spp. (−16%, <i>p</i> = 0.045), and an increase in <i>Lactobacillus</i> (+3%, <i>p</i> = 0.045) and in less abundant genera belonging to the <i>Enterobacteriales</i> family were found. An increase in alpha diversity (Shannon’s and Simpson’s indexes) and significant alterations in beta diversity were observed. A correlation of necrotizing enterocolitis with higher <i>Staphylococcus</i> abundance and higher alpha diversity was also observed. H2-blockers and/or proton pump inhibitor therapy were positively correlated with a higher total bacteria number. In conclusion, these results suggest that positive changes occur in the gut microbiota profile of infants three weeks after intestinal surgery. |
| first_indexed | 2024-03-10T21:02:10Z |
| format | Article |
| id | doaj.art-f7197147f6854d6e89b77729c54d9988 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T21:02:10Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-f7197147f6854d6e89b77729c54d99882023-11-19T17:27:26ZengMDPI AGMicroorganisms2076-26072023-10-011110248210.3390/microorganisms11102482Changes in Microbiota Profile in the Proximal Remnant Intestine in Infants Undergoing Surgery Requiring EnterostomyInês Barreiros-Mota0João R. Araújo1Cláudia Marques2Laura Sousa3Juliana Morais4Inês Castela5Ana Faria6Maria Teresa Neto7Gonçalo Cordeiro-Ferreira8Daniel Virella9Ana Pita10Luís Pereira-da-Silva11Conceição Calhau12Nutrition & Metabolism Department, NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM), Universidade NOVA de Lisboa, 1169-056 Lisbon, PortugalNutrition & Metabolism Department, NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM), Universidade NOVA de Lisboa, 1169-056 Lisbon, PortugalNutrition & Metabolism Department, NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM), Universidade NOVA de Lisboa, 1169-056 Lisbon, PortugalNutrition & Metabolism Department, NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM), Universidade NOVA de Lisboa, 1169-056 Lisbon, PortugalCHRC—Comprehensive Health Research Centre, NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM), Universidade Nova de Lisboa, 1169-056 Lisbon, PortugalNutrition & Metabolism Department, NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM), Universidade NOVA de Lisboa, 1169-056 Lisbon, PortugalNutrition & Metabolism Department, NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM), Universidade NOVA de Lisboa, 1169-056 Lisbon, PortugalCHRC—Comprehensive Health Research Centre, NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM), Universidade Nova de Lisboa, 1169-056 Lisbon, PortugalNeonatal Intensive Care Unit, Department of Pediatrics, Hospital Dona Estefânia, Centro Hospitalar Universitário de Lisboa Central, 1169-045 Lisbon, PortugalNeonatal Intensive Care Unit, Department of Pediatrics, Hospital Dona Estefânia, Centro Hospitalar Universitário de Lisboa Central, 1169-045 Lisbon, PortugalNeonatal Intensive Care Unit, Department of Pediatrics, Hospital Dona Estefânia, Centro Hospitalar Universitário de Lisboa Central, 1169-045 Lisbon, PortugalCHRC—Comprehensive Health Research Centre, NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM), Universidade Nova de Lisboa, 1169-056 Lisbon, PortugalNutrition & Metabolism Department, NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM), Universidade NOVA de Lisboa, 1169-056 Lisbon, PortugalEarly-life gut dysbiosis has been associated with an increased risk of inflammatory, metabolic, and immune diseases later in life. Data on gut microbiota changes in infants undergoing intestinal surgery requiring enterostomy are scarce. This prospective cohort study examined the enterostomy effluent of 29 infants who underwent intestinal surgery due to congenital malformations of the gastrointestinal tract, necrotizing enterocolitis, or spontaneous intestinal perforation. Initial effluent samples were collected immediately after surgery and final effluent samples were collected three weeks later. Gut microbiota composition was analysed using real-time PCR and 16S rRNA gene sequencing. Three weeks after surgery, an increase in total bacteria number (+21%, <i>p</i> = 0.026), a decrease in <i>Staphylococcus</i> (−21%, <i>p</i> = 0.002) and <i>Candida</i> spp. (−16%, <i>p</i> = 0.045), and an increase in <i>Lactobacillus</i> (+3%, <i>p</i> = 0.045) and in less abundant genera belonging to the <i>Enterobacteriales</i> family were found. An increase in alpha diversity (Shannon’s and Simpson’s indexes) and significant alterations in beta diversity were observed. A correlation of necrotizing enterocolitis with higher <i>Staphylococcus</i> abundance and higher alpha diversity was also observed. H2-blockers and/or proton pump inhibitor therapy were positively correlated with a higher total bacteria number. In conclusion, these results suggest that positive changes occur in the gut microbiota profile of infants three weeks after intestinal surgery.https://www.mdpi.com/2076-2607/11/10/2482enterostomyinfantsmicrobiotanecrotizing enterocolitisprematurity |
| spellingShingle | Inês Barreiros-Mota João R. Araújo Cláudia Marques Laura Sousa Juliana Morais Inês Castela Ana Faria Maria Teresa Neto Gonçalo Cordeiro-Ferreira Daniel Virella Ana Pita Luís Pereira-da-Silva Conceição Calhau Changes in Microbiota Profile in the Proximal Remnant Intestine in Infants Undergoing Surgery Requiring Enterostomy Microorganisms enterostomy infants microbiota necrotizing enterocolitis prematurity |
| title | Changes in Microbiota Profile in the Proximal Remnant Intestine in Infants Undergoing Surgery Requiring Enterostomy |
| title_full | Changes in Microbiota Profile in the Proximal Remnant Intestine in Infants Undergoing Surgery Requiring Enterostomy |
| title_fullStr | Changes in Microbiota Profile in the Proximal Remnant Intestine in Infants Undergoing Surgery Requiring Enterostomy |
| title_full_unstemmed | Changes in Microbiota Profile in the Proximal Remnant Intestine in Infants Undergoing Surgery Requiring Enterostomy |
| title_short | Changes in Microbiota Profile in the Proximal Remnant Intestine in Infants Undergoing Surgery Requiring Enterostomy |
| title_sort | changes in microbiota profile in the proximal remnant intestine in infants undergoing surgery requiring enterostomy |
| topic | enterostomy infants microbiota necrotizing enterocolitis prematurity |
| url | https://www.mdpi.com/2076-2607/11/10/2482 |
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