Artificial RNA Motifs Expand the Programmable Assembly between RNA Modules of a Bimolecular Ribozyme Leading to Application to RNA Nanostructure Design
A bimolecular ribozyme consisting of a core ribozyme (ΔP5 RNA) and an activator module (P5abc RNA) has been used as a platform to design assembled RNA nanostructures. The tight and specific assembly between the P5abc and ΔP5 modules depends on two sets of intermodule interactions. The interface betw...
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| Format: | Article |
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MDPI AG
2017-10-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/6/4/37 |
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| author | Md. Motiar Rahman Shigeyoshi Matsumura Yoshiya Ikawa |
| author_facet | Md. Motiar Rahman Shigeyoshi Matsumura Yoshiya Ikawa |
| author_sort | Md. Motiar Rahman |
| collection | DOAJ |
| description | A bimolecular ribozyme consisting of a core ribozyme (ΔP5 RNA) and an activator module (P5abc RNA) has been used as a platform to design assembled RNA nanostructures. The tight and specific assembly between the P5abc and ΔP5 modules depends on two sets of intermodule interactions. The interface between P5abc and ΔP5 must be controlled when designing RNA nanostructures. To expand the repertoire of molecular recognition in the P5abc/ΔP5 interface, we modified the interface by replacing the parent tertiary interactions in the interface with artificial interactions. The engineered P5abc/ΔP5 interfaces were characterized biochemically to identify those suitable for nanostructure design. The new interfaces were used to construct 2D-square and 1D-array RNA nanostructures. |
| first_indexed | 2024-03-12T10:28:20Z |
| format | Article |
| id | doaj.art-f71a34732b90445a933180f638db15e4 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-12T10:28:20Z |
| publishDate | 2017-10-01 |
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| series | Biology |
| spelling | doaj.art-f71a34732b90445a933180f638db15e42023-09-02T09:33:08ZengMDPI AGBiology2079-77372017-10-01643710.3390/biology6040037biology6040037Artificial RNA Motifs Expand the Programmable Assembly between RNA Modules of a Bimolecular Ribozyme Leading to Application to RNA Nanostructure DesignMd. Motiar Rahman0Shigeyoshi Matsumura1Yoshiya Ikawa2Department of Chemistry, Graduate School of Science and Engineering, University of Toyama, Gofuku 3190, Toyama 9308555, JapanDepartment of Chemistry, Graduate School of Science and Engineering, University of Toyama, Gofuku 3190, Toyama 9308555, JapanDepartment of Chemistry, Graduate School of Science and Engineering, University of Toyama, Gofuku 3190, Toyama 9308555, JapanA bimolecular ribozyme consisting of a core ribozyme (ΔP5 RNA) and an activator module (P5abc RNA) has been used as a platform to design assembled RNA nanostructures. The tight and specific assembly between the P5abc and ΔP5 modules depends on two sets of intermodule interactions. The interface between P5abc and ΔP5 must be controlled when designing RNA nanostructures. To expand the repertoire of molecular recognition in the P5abc/ΔP5 interface, we modified the interface by replacing the parent tertiary interactions in the interface with artificial interactions. The engineered P5abc/ΔP5 interfaces were characterized biochemically to identify those suitable for nanostructure design. The new interfaces were used to construct 2D-square and 1D-array RNA nanostructures.https://www.mdpi.com/2079-7737/6/4/37catalytic RNAgroup I intronribozymeRNA nanostructureRNA nanotechnology |
| spellingShingle | Md. Motiar Rahman Shigeyoshi Matsumura Yoshiya Ikawa Artificial RNA Motifs Expand the Programmable Assembly between RNA Modules of a Bimolecular Ribozyme Leading to Application to RNA Nanostructure Design Biology catalytic RNA group I intron ribozyme RNA nanostructure RNA nanotechnology |
| title | Artificial RNA Motifs Expand the Programmable Assembly between RNA Modules of a Bimolecular Ribozyme Leading to Application to RNA Nanostructure Design |
| title_full | Artificial RNA Motifs Expand the Programmable Assembly between RNA Modules of a Bimolecular Ribozyme Leading to Application to RNA Nanostructure Design |
| title_fullStr | Artificial RNA Motifs Expand the Programmable Assembly between RNA Modules of a Bimolecular Ribozyme Leading to Application to RNA Nanostructure Design |
| title_full_unstemmed | Artificial RNA Motifs Expand the Programmable Assembly between RNA Modules of a Bimolecular Ribozyme Leading to Application to RNA Nanostructure Design |
| title_short | Artificial RNA Motifs Expand the Programmable Assembly between RNA Modules of a Bimolecular Ribozyme Leading to Application to RNA Nanostructure Design |
| title_sort | artificial rna motifs expand the programmable assembly between rna modules of a bimolecular ribozyme leading to application to rna nanostructure design |
| topic | catalytic RNA group I intron ribozyme RNA nanostructure RNA nanotechnology |
| url | https://www.mdpi.com/2079-7737/6/4/37 |
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