Influence of Polysaccharides From Polygonatum kingianum on Short-Chain Fatty Acid Production and Quorum Sensing in Lactobacillus faecis
Polysaccharide is one of the main active ingredients of Polygonatum kingianum, which has been proven to regulate the balance of gut microbiota. For the first time, this study focused on the regulation of polysaccharides from Polygonatum kingianum (PS) on Lactobacillus faecis, a specific probiotic in...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-11-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.758870/full |
| _version_ | 1819003787529945088 |
|---|---|
| author | Min Yang Min Yang Fanying Meng Wen Gu Lihui Fu Fan Zhang Fengjiao Li Yating Tao Zhengyang Zhang Xi Wang Xingxin Yang Jingping Li Jie Yu |
| author_facet | Min Yang Min Yang Fanying Meng Wen Gu Lihui Fu Fan Zhang Fengjiao Li Yating Tao Zhengyang Zhang Xi Wang Xingxin Yang Jingping Li Jie Yu |
| author_sort | Min Yang |
| collection | DOAJ |
| description | Polysaccharide is one of the main active ingredients of Polygonatum kingianum, which has been proven to regulate the balance of gut microbiota. For the first time, this study focused on the regulation of polysaccharides from Polygonatum kingianum (PS) on Lactobacillus faecis, a specific probiotic in the intestinal tract. PS effectively promoted the biomass, biofilm and acetic acid production in L. faecis 2-84, and enhanced quorum sensing (QS) signaling. The characteristics of gene sequence were analyzed using genomics approaches, and L. faecis 2-84 was found to encode 18 genes that are closely related to QS and 10 genes related to short-chain fatty acids (SCFAs). Additionally, transcriptome and proteome analysis demonstrated that PS could promote the QS system of L. faecis by enhancing the transcription of oppA gene and expression of oppD protein. PS also regulated the production and metabolism of SCFAs of L. faecis by upregulating the expression of ldh and metE gene and adh2 protein, and downregulating the expression of mvK gene. In conclusion, it was speculated that PS could affect intestinal SCFAs production by affecting the QS system and SCFAs production in L. faecis. The present study implied that PS might have a role in promoting the growth of intestinal probiotics, where the QS system and SCFAs might be two of the important mechanisms for the probiotic activity of PS. |
| first_indexed | 2024-12-20T23:26:33Z |
| format | Article |
| id | doaj.art-f71a6920b14a4414863ce2fbbe0bed00 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-20T23:26:33Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-f71a6920b14a4414863ce2fbbe0bed002022-12-21T19:23:23ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-11-011210.3389/fmicb.2021.758870758870Influence of Polysaccharides From Polygonatum kingianum on Short-Chain Fatty Acid Production and Quorum Sensing in Lactobacillus faecisMin Yang0Min Yang1Fanying Meng2Wen Gu3Lihui Fu4Fan Zhang5Fengjiao Li6Yating Tao7Zhengyang Zhang8Xi Wang9Xingxin Yang10Jingping Li11Jie Yu12Yunnan Key Laboratory of Southern Medicine Utilization, College of Pharmaceutical Science, Yunnan University of Chinese Medicine, Kunming, ChinaKunming Third People’s Hospital, Kunming, ChinaYunnan Key Laboratory of Southern Medicine Utilization, College of Pharmaceutical Science, Yunnan University of Chinese Medicine, Kunming, ChinaYunnan Key Laboratory of Southern Medicine Utilization, College of Pharmaceutical Science, Yunnan University of Chinese Medicine, Kunming, ChinaYunnan Key Laboratory of Southern Medicine Utilization, College of Pharmaceutical Science, Yunnan University of Chinese Medicine, Kunming, ChinaYunnan Key Laboratory of Southern Medicine Utilization, College of Pharmaceutical Science, Yunnan University of Chinese Medicine, Kunming, ChinaYunnan Key Laboratory of Southern Medicine Utilization, College of Pharmaceutical Science, Yunnan University of Chinese Medicine, Kunming, ChinaYunnan Key Laboratory of Southern Medicine Utilization, College of Pharmaceutical Science, Yunnan University of Chinese Medicine, Kunming, ChinaYunnan Key Laboratory of Southern Medicine Utilization, College of Pharmaceutical Science, Yunnan University of Chinese Medicine, Kunming, ChinaYunnan Key Laboratory of Southern Medicine Utilization, College of Pharmaceutical Science, Yunnan University of Chinese Medicine, Kunming, ChinaYunnan Key Laboratory of Southern Medicine Utilization, College of Pharmaceutical Science, Yunnan University of Chinese Medicine, Kunming, ChinaYunnan Key Laboratory of Southern Medicine Utilization, College of Pharmaceutical Science, Yunnan University of Chinese Medicine, Kunming, ChinaYunnan Key Laboratory of Southern Medicine Utilization, College of Pharmaceutical Science, Yunnan University of Chinese Medicine, Kunming, ChinaPolysaccharide is one of the main active ingredients of Polygonatum kingianum, which has been proven to regulate the balance of gut microbiota. For the first time, this study focused on the regulation of polysaccharides from Polygonatum kingianum (PS) on Lactobacillus faecis, a specific probiotic in the intestinal tract. PS effectively promoted the biomass, biofilm and acetic acid production in L. faecis 2-84, and enhanced quorum sensing (QS) signaling. The characteristics of gene sequence were analyzed using genomics approaches, and L. faecis 2-84 was found to encode 18 genes that are closely related to QS and 10 genes related to short-chain fatty acids (SCFAs). Additionally, transcriptome and proteome analysis demonstrated that PS could promote the QS system of L. faecis by enhancing the transcription of oppA gene and expression of oppD protein. PS also regulated the production and metabolism of SCFAs of L. faecis by upregulating the expression of ldh and metE gene and adh2 protein, and downregulating the expression of mvK gene. In conclusion, it was speculated that PS could affect intestinal SCFAs production by affecting the QS system and SCFAs production in L. faecis. The present study implied that PS might have a role in promoting the growth of intestinal probiotics, where the QS system and SCFAs might be two of the important mechanisms for the probiotic activity of PS.https://www.frontiersin.org/articles/10.3389/fmicb.2021.758870/fullLactobacillus faecisquorum sensingshort-chain fatty acidspolysaccharidesPolygonatum kingianum |
| spellingShingle | Min Yang Min Yang Fanying Meng Wen Gu Lihui Fu Fan Zhang Fengjiao Li Yating Tao Zhengyang Zhang Xi Wang Xingxin Yang Jingping Li Jie Yu Influence of Polysaccharides From Polygonatum kingianum on Short-Chain Fatty Acid Production and Quorum Sensing in Lactobacillus faecis Frontiers in Microbiology Lactobacillus faecis quorum sensing short-chain fatty acids polysaccharides Polygonatum kingianum |
| title | Influence of Polysaccharides From Polygonatum kingianum on Short-Chain Fatty Acid Production and Quorum Sensing in Lactobacillus faecis |
| title_full | Influence of Polysaccharides From Polygonatum kingianum on Short-Chain Fatty Acid Production and Quorum Sensing in Lactobacillus faecis |
| title_fullStr | Influence of Polysaccharides From Polygonatum kingianum on Short-Chain Fatty Acid Production and Quorum Sensing in Lactobacillus faecis |
| title_full_unstemmed | Influence of Polysaccharides From Polygonatum kingianum on Short-Chain Fatty Acid Production and Quorum Sensing in Lactobacillus faecis |
| title_short | Influence of Polysaccharides From Polygonatum kingianum on Short-Chain Fatty Acid Production and Quorum Sensing in Lactobacillus faecis |
| title_sort | influence of polysaccharides from polygonatum kingianum on short chain fatty acid production and quorum sensing in lactobacillus faecis |
| topic | Lactobacillus faecis quorum sensing short-chain fatty acids polysaccharides Polygonatum kingianum |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.758870/full |
| work_keys_str_mv | AT minyang influenceofpolysaccharidesfrompolygonatumkingianumonshortchainfattyacidproductionandquorumsensinginlactobacillusfaecis AT minyang influenceofpolysaccharidesfrompolygonatumkingianumonshortchainfattyacidproductionandquorumsensinginlactobacillusfaecis AT fanyingmeng influenceofpolysaccharidesfrompolygonatumkingianumonshortchainfattyacidproductionandquorumsensinginlactobacillusfaecis AT wengu influenceofpolysaccharidesfrompolygonatumkingianumonshortchainfattyacidproductionandquorumsensinginlactobacillusfaecis AT lihuifu influenceofpolysaccharidesfrompolygonatumkingianumonshortchainfattyacidproductionandquorumsensinginlactobacillusfaecis AT fanzhang influenceofpolysaccharidesfrompolygonatumkingianumonshortchainfattyacidproductionandquorumsensinginlactobacillusfaecis AT fengjiaoli influenceofpolysaccharidesfrompolygonatumkingianumonshortchainfattyacidproductionandquorumsensinginlactobacillusfaecis AT yatingtao influenceofpolysaccharidesfrompolygonatumkingianumonshortchainfattyacidproductionandquorumsensinginlactobacillusfaecis AT zhengyangzhang influenceofpolysaccharidesfrompolygonatumkingianumonshortchainfattyacidproductionandquorumsensinginlactobacillusfaecis AT xiwang influenceofpolysaccharidesfrompolygonatumkingianumonshortchainfattyacidproductionandquorumsensinginlactobacillusfaecis AT xingxinyang influenceofpolysaccharidesfrompolygonatumkingianumonshortchainfattyacidproductionandquorumsensinginlactobacillusfaecis AT jingpingli influenceofpolysaccharidesfrompolygonatumkingianumonshortchainfattyacidproductionandquorumsensinginlactobacillusfaecis AT jieyu influenceofpolysaccharidesfrompolygonatumkingianumonshortchainfattyacidproductionandquorumsensinginlactobacillusfaecis |