Longitudinal Testing of <i>Leptospira</i> Antibodies in Horses Located near a Leptospirosis Outbreak in Alpacas
The objectives of this study were to determine if horses located near an outbreak of leptospirosis in alpacas had <i>Leptospira</i> titres indicative of a previous or current infection and, if so, to determine the magnitude in change of titres over time. Further, the objective was to det...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/9/8/426 |
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| author | Charlotte Bolwell Erica Gee Brooke Adams Julie Collins-Emerson Katherine Scarfe Shahista Nisa Emma Gordon Chris Rogers Jackie Benschop |
| author_facet | Charlotte Bolwell Erica Gee Brooke Adams Julie Collins-Emerson Katherine Scarfe Shahista Nisa Emma Gordon Chris Rogers Jackie Benschop |
| author_sort | Charlotte Bolwell |
| collection | DOAJ |
| description | The objectives of this study were to determine if horses located near an outbreak of leptospirosis in alpacas had <i>Leptospira</i> titres indicative of a previous or current infection and, if so, to determine the magnitude in change of titres over time. Further, the objective was to determine if horses with high titre results were shedding <i>Leptospira</i> in their urine. Blood samples were collected from twelve horses located on or next to the farm with the outbreak in alpacas, on day zero and at four subsequent time points (two, four, six and nine weeks). The microscopic agglutination test was used to test sera for five serovars endemic in New Zealand: Ballum, Copenhageni, Hardjo, Pomona and Tarassovi. A reciprocal MAT titre cut-off of ≥1:100 was used to determine positive horses. Seven out of twelve horses (58%) were positive to at least one serovar during one of the time points. Two horses recorded titres of ≥1600, one for both Pomona and Copenhageni and the other for Hardjo, and these two horses were both PCR positive for <i>Leptospira</i> in their urine samples. For five out of seven horses, the titres either remained the same or changed by one dilution across the sampling time points. The study confirmed endemic exposure to five endemic <i>Leptospira</i> serovars in New Zealand in a group of horses located near a confirmed leptospirosis outbreak in alpacas. |
| first_indexed | 2024-03-09T09:47:26Z |
| format | Article |
| id | doaj.art-f71b89862b134cde97d6a7f3ec12031d |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-09T09:47:26Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-f71b89862b134cde97d6a7f3ec12031d2023-12-02T00:27:29ZengMDPI AGVeterinary Sciences2306-73812022-08-019842610.3390/vetsci9080426Longitudinal Testing of <i>Leptospira</i> Antibodies in Horses Located near a Leptospirosis Outbreak in AlpacasCharlotte Bolwell0Erica Gee1Brooke Adams2Julie Collins-Emerson3Katherine Scarfe4Shahista Nisa5Emma Gordon6Chris Rogers7Jackie Benschop8School of Veterinary Science, Massey University, Palmerston North 4442, New ZealandSchool of Veterinary Science, Massey University, Palmerston North 4442, New ZealandSchool of Veterinary Science, Massey University, Palmerston North 4442, New ZealandSchool of Veterinary Science, Massey University, Palmerston North 4442, New ZealandIDEXX Laboratories (NZ) ULC, School of Veterinary Science Complex, Massey University, Palmerston North 4442, New ZealandSchool of Veterinary Science, Massey University, Palmerston North 4442, New ZealandSchool of Veterinary Science, Massey University, Palmerston North 4442, New ZealandSchool of Veterinary Science, Massey University, Palmerston North 4442, New ZealandSchool of Veterinary Science, Massey University, Palmerston North 4442, New ZealandThe objectives of this study were to determine if horses located near an outbreak of leptospirosis in alpacas had <i>Leptospira</i> titres indicative of a previous or current infection and, if so, to determine the magnitude in change of titres over time. Further, the objective was to determine if horses with high titre results were shedding <i>Leptospira</i> in their urine. Blood samples were collected from twelve horses located on or next to the farm with the outbreak in alpacas, on day zero and at four subsequent time points (two, four, six and nine weeks). The microscopic agglutination test was used to test sera for five serovars endemic in New Zealand: Ballum, Copenhageni, Hardjo, Pomona and Tarassovi. A reciprocal MAT titre cut-off of ≥1:100 was used to determine positive horses. Seven out of twelve horses (58%) were positive to at least one serovar during one of the time points. Two horses recorded titres of ≥1600, one for both Pomona and Copenhageni and the other for Hardjo, and these two horses were both PCR positive for <i>Leptospira</i> in their urine samples. For five out of seven horses, the titres either remained the same or changed by one dilution across the sampling time points. The study confirmed endemic exposure to five endemic <i>Leptospira</i> serovars in New Zealand in a group of horses located near a confirmed leptospirosis outbreak in alpacas.https://www.mdpi.com/2306-7381/9/8/426horse<i>Leptospira</i> serovarsleptospirosisNew Zealand |
| spellingShingle | Charlotte Bolwell Erica Gee Brooke Adams Julie Collins-Emerson Katherine Scarfe Shahista Nisa Emma Gordon Chris Rogers Jackie Benschop Longitudinal Testing of <i>Leptospira</i> Antibodies in Horses Located near a Leptospirosis Outbreak in Alpacas Veterinary Sciences horse <i>Leptospira</i> serovars leptospirosis New Zealand |
| title | Longitudinal Testing of <i>Leptospira</i> Antibodies in Horses Located near a Leptospirosis Outbreak in Alpacas |
| title_full | Longitudinal Testing of <i>Leptospira</i> Antibodies in Horses Located near a Leptospirosis Outbreak in Alpacas |
| title_fullStr | Longitudinal Testing of <i>Leptospira</i> Antibodies in Horses Located near a Leptospirosis Outbreak in Alpacas |
| title_full_unstemmed | Longitudinal Testing of <i>Leptospira</i> Antibodies in Horses Located near a Leptospirosis Outbreak in Alpacas |
| title_short | Longitudinal Testing of <i>Leptospira</i> Antibodies in Horses Located near a Leptospirosis Outbreak in Alpacas |
| title_sort | longitudinal testing of i leptospira i antibodies in horses located near a leptospirosis outbreak in alpacas |
| topic | horse <i>Leptospira</i> serovars leptospirosis New Zealand |
| url | https://www.mdpi.com/2306-7381/9/8/426 |
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