Extraction of Bioactive Compounds from Red Cardinal Grape Pomace by Deep Eutectic Solvents

The objective of this study was to determine the most suitable extraction conditions from Red cardinal grape pomace using deep eutectic solvents (DES). Factors influencing the extraction process were investigated, including DES type, the ratio of hydrogen bond acceptor (HBA) to hydrogen bond donor (...

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Main Authors: Thao T.T. Nguyen, Viet B. Nguyen, Truc M. X. Le, An H.T. Tran, Tuyen C. Kha
Format: Article
Language:English
Published: AIDIC Servizi S.r.l. 2023-12-01
Series:Chemical Engineering Transactions
Online Access:https://www.cetjournal.it/index.php/cet/article/view/14209
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author Thao T.T. Nguyen
Viet B. Nguyen
Truc M. X. Le
An H.T. Tran
Tuyen C. Kha
author_facet Thao T.T. Nguyen
Viet B. Nguyen
Truc M. X. Le
An H.T. Tran
Tuyen C. Kha
author_sort Thao T.T. Nguyen
collection DOAJ
description The objective of this study was to determine the most suitable extraction conditions from Red cardinal grape pomace using deep eutectic solvents (DES). Factors influencing the extraction process were investigated, including DES type, the ratio of hydrogen bond acceptor (HBA) to hydrogen bond donor (HBD), water addition, solvent-to-material ratio, extraction temperature, and time. The best results were obtained with choline chloride: glycerol: citric acid (1:1:1, w/w/w) DES, adding 25 % water, using a 25:1 (v/w) solvent-to-material ratio, 60 °C, and 2.5 h extraction. The grape pomace extract showed substantial levels of bioactive compounds, with the highest recorded values being 65.49 ± 1.30 mg GAE/g DW for total polyphenol content, 177.88 ± 5.17 mg AE/g DW for total triterpenoid saponin content, and 36.22 ± 0.77 mg CE/g DW for proanthocyanidin content. The concentrations of specific compounds were quantified, revealing DES's superior extraction compared to ethanol and methanol, highlighting DES's effectiveness as an alternative extraction solvent.
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spelling doaj.art-f71e7ef72bd14d38ac58f56fc25fb3f12023-12-15T23:50:30ZengAIDIC Servizi S.r.l.Chemical Engineering Transactions2283-92162023-12-0110610.3303/CET23106149Extraction of Bioactive Compounds from Red Cardinal Grape Pomace by Deep Eutectic SolventsThao T.T. NguyenViet B. NguyenTruc M. X. LeAn H.T. TranTuyen C. KhaThe objective of this study was to determine the most suitable extraction conditions from Red cardinal grape pomace using deep eutectic solvents (DES). Factors influencing the extraction process were investigated, including DES type, the ratio of hydrogen bond acceptor (HBA) to hydrogen bond donor (HBD), water addition, solvent-to-material ratio, extraction temperature, and time. The best results were obtained with choline chloride: glycerol: citric acid (1:1:1, w/w/w) DES, adding 25 % water, using a 25:1 (v/w) solvent-to-material ratio, 60 °C, and 2.5 h extraction. The grape pomace extract showed substantial levels of bioactive compounds, with the highest recorded values being 65.49 ± 1.30 mg GAE/g DW for total polyphenol content, 177.88 ± 5.17 mg AE/g DW for total triterpenoid saponin content, and 36.22 ± 0.77 mg CE/g DW for proanthocyanidin content. The concentrations of specific compounds were quantified, revealing DES's superior extraction compared to ethanol and methanol, highlighting DES's effectiveness as an alternative extraction solvent.https://www.cetjournal.it/index.php/cet/article/view/14209
spellingShingle Thao T.T. Nguyen
Viet B. Nguyen
Truc M. X. Le
An H.T. Tran
Tuyen C. Kha
Extraction of Bioactive Compounds from Red Cardinal Grape Pomace by Deep Eutectic Solvents
Chemical Engineering Transactions
title Extraction of Bioactive Compounds from Red Cardinal Grape Pomace by Deep Eutectic Solvents
title_full Extraction of Bioactive Compounds from Red Cardinal Grape Pomace by Deep Eutectic Solvents
title_fullStr Extraction of Bioactive Compounds from Red Cardinal Grape Pomace by Deep Eutectic Solvents
title_full_unstemmed Extraction of Bioactive Compounds from Red Cardinal Grape Pomace by Deep Eutectic Solvents
title_short Extraction of Bioactive Compounds from Red Cardinal Grape Pomace by Deep Eutectic Solvents
title_sort extraction of bioactive compounds from red cardinal grape pomace by deep eutectic solvents
url https://www.cetjournal.it/index.php/cet/article/view/14209
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