Adjunctive Antiseptic Irrigation of Periodontal Pockets: Effects on Microbial and Cytokine Profiles
To evaluate the effect of adjunctive antiseptic irrigation of periodontal pockets on microbial and cytokine profiles. Fifty-nine patients with severe periodontitis were allocated to one of three groups for scaling and root planing facilitated with different adjunctive antiseptics: 1% polyhexamethyle...
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MDPI AG
2020-11-01
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| Series: | Dentistry Journal |
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| Online Access: | https://www.mdpi.com/2304-6767/8/4/124 |
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| author | Anton Vitt Andrei Babenka Elisabeth A. Boström Anders Gustafsson Ronaldo Lira Junior Veronica Slizen Timo Sorsa Taina Tervahartiala Kåre Buhlin |
| author_facet | Anton Vitt Andrei Babenka Elisabeth A. Boström Anders Gustafsson Ronaldo Lira Junior Veronica Slizen Timo Sorsa Taina Tervahartiala Kåre Buhlin |
| author_sort | Anton Vitt |
| collection | DOAJ |
| description | To evaluate the effect of adjunctive antiseptic irrigation of periodontal pockets on microbial and cytokine profiles. Fifty-nine patients with severe periodontitis were allocated to one of three groups for scaling and root planing facilitated with different adjunctive antiseptics: 1% polyhexamethyleneguanidine phosphate (PHMG-P) (n = 19), 0.2% chlorhexidine (CHX) (n = 21) or distilled water (n = 19). Gingival crevicular fluid and subgingival bacterial samples were collected at baseline, and at 2 weeks, and 1 and 4 months. The levels of interleukin (IL)-1β, IL-8, IL-10, and IL-17A, matrix metalloproteinase (MMP)-8, <i>Porphyromonas gingivalis</i>, <i>Tannerella forsythia</i>, <i>Treponema denticola</i>, <i>Fusobacterium nucleatum,</i><i>Aggregatibacter actinomycetemcomitans</i>, and <i>Prevotella intermedia</i> were determined. There were no intergroup differences in cytokine concentrations and bacterial counts at any follow-up, however, varying patterns were observed. In the PHMG-P and water groups IL-1β expression peaked at 2 weeks and then gradually declined. In all three groups, the dynamics of MMP-8 concentration were non-linear, increasing by 2 weeks and then declining to below baseline (<i>p</i> > 0.05). <i>P. gingivalis</i> and <i>T. forsythia</i> declined within the first month and increased thereafter, not regaining the baseline level. Adjunctive antiseptic treatment was associated with changes in biomarkers and bacterial counts in the course of the study. The effects of adjunctive antiseptic irrigation were limited in the applied protocol. |
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| id | doaj.art-f71ee0aeee734ea7b87ff0d59472a633 |
| institution | Directory Open Access Journal |
| issn | 2304-6767 |
| language | English |
| last_indexed | 2024-03-10T15:09:33Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Dentistry Journal |
| spelling | doaj.art-f71ee0aeee734ea7b87ff0d59472a6332023-11-20T19:28:20ZengMDPI AGDentistry Journal2304-67672020-11-018412410.3390/dj8040124Adjunctive Antiseptic Irrigation of Periodontal Pockets: Effects on Microbial and Cytokine ProfilesAnton Vitt0Andrei Babenka1Elisabeth A. Boström2Anders Gustafsson3Ronaldo Lira Junior4Veronica Slizen5Timo Sorsa6Taina Tervahartiala7Kåre Buhlin8Department of Dental Medicine, Division of Periodontology, Karolinska Institutet, 14152 Huddinge, SwedenDepartment of Bioorganic Chemistry, Belarusian State Medical University, 220045 Minsk, BelarusDepartment of Dental Medicine, Division of Periodontology, Karolinska Institutet, 14152 Huddinge, SwedenDepartment of Dental Medicine, Division of Periodontology, Karolinska Institutet, 14152 Huddinge, SwedenDepartment of Dental Medicine, Division of Periodontology, Karolinska Institutet, 14152 Huddinge, SwedenDepartment of Microbiology, Virology and Immunology, Belarusian State Medical University, 220045 Minsk, BelarusDepartment of Dental Medicine, Division of Periodontology, Karolinska Institutet, 14152 Huddinge, SwedenDepartment of Oral and Maxillofacial Diseases, Institute of Dentistry, University of Helsinki, 00290 Helsinki, FinlandDepartment of Dental Medicine, Division of Periodontology, Karolinska Institutet, 14152 Huddinge, SwedenTo evaluate the effect of adjunctive antiseptic irrigation of periodontal pockets on microbial and cytokine profiles. Fifty-nine patients with severe periodontitis were allocated to one of three groups for scaling and root planing facilitated with different adjunctive antiseptics: 1% polyhexamethyleneguanidine phosphate (PHMG-P) (n = 19), 0.2% chlorhexidine (CHX) (n = 21) or distilled water (n = 19). Gingival crevicular fluid and subgingival bacterial samples were collected at baseline, and at 2 weeks, and 1 and 4 months. The levels of interleukin (IL)-1β, IL-8, IL-10, and IL-17A, matrix metalloproteinase (MMP)-8, <i>Porphyromonas gingivalis</i>, <i>Tannerella forsythia</i>, <i>Treponema denticola</i>, <i>Fusobacterium nucleatum,</i><i>Aggregatibacter actinomycetemcomitans</i>, and <i>Prevotella intermedia</i> were determined. There were no intergroup differences in cytokine concentrations and bacterial counts at any follow-up, however, varying patterns were observed. In the PHMG-P and water groups IL-1β expression peaked at 2 weeks and then gradually declined. In all three groups, the dynamics of MMP-8 concentration were non-linear, increasing by 2 weeks and then declining to below baseline (<i>p</i> > 0.05). <i>P. gingivalis</i> and <i>T. forsythia</i> declined within the first month and increased thereafter, not regaining the baseline level. Adjunctive antiseptic treatment was associated with changes in biomarkers and bacterial counts in the course of the study. The effects of adjunctive antiseptic irrigation were limited in the applied protocol.https://www.mdpi.com/2304-6767/8/4/124inflammationperiodontitischlorhexidinepolyhexamethylene guanidine phosphateirrigation |
| spellingShingle | Anton Vitt Andrei Babenka Elisabeth A. Boström Anders Gustafsson Ronaldo Lira Junior Veronica Slizen Timo Sorsa Taina Tervahartiala Kåre Buhlin Adjunctive Antiseptic Irrigation of Periodontal Pockets: Effects on Microbial and Cytokine Profiles Dentistry Journal inflammation periodontitis chlorhexidine polyhexamethylene guanidine phosphate irrigation |
| title | Adjunctive Antiseptic Irrigation of Periodontal Pockets: Effects on Microbial and Cytokine Profiles |
| title_full | Adjunctive Antiseptic Irrigation of Periodontal Pockets: Effects on Microbial and Cytokine Profiles |
| title_fullStr | Adjunctive Antiseptic Irrigation of Periodontal Pockets: Effects on Microbial and Cytokine Profiles |
| title_full_unstemmed | Adjunctive Antiseptic Irrigation of Periodontal Pockets: Effects on Microbial and Cytokine Profiles |
| title_short | Adjunctive Antiseptic Irrigation of Periodontal Pockets: Effects on Microbial and Cytokine Profiles |
| title_sort | adjunctive antiseptic irrigation of periodontal pockets effects on microbial and cytokine profiles |
| topic | inflammation periodontitis chlorhexidine polyhexamethylene guanidine phosphate irrigation |
| url | https://www.mdpi.com/2304-6767/8/4/124 |
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