Corticosterone Can Be an Essential Stress Index in Channel Catfish (Ictalurus punctatus)
Although cortisol is considered the main glucocorticoid involved in regulating stress in fish by elevating the serum glucose, the presence of serum corticosterone and whether its level can be used as an indicator for fish stress activation and reflect the degree of fish stress remains to be determin...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-08-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2021.692726/full |
| _version_ | 1818356270256619520 |
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| author | Kang Xiao Kang Xiao Xi Wang Xi Wang Wen-Bin Liu Wen-Bin Liu Ding-Dong Zhang Ding-Dong Zhang Xiang-Fei Li Xiang-Fei Li Chun-Nuan Zhang Chun-Nuan Zhang Wei-Hua Chen Wei-Hua Chen Kenneth Prudence Abasubong Kenneth Prudence Abasubong Guang-Zhen Jiang Guang-Zhen Jiang |
| author_facet | Kang Xiao Kang Xiao Xi Wang Xi Wang Wen-Bin Liu Wen-Bin Liu Ding-Dong Zhang Ding-Dong Zhang Xiang-Fei Li Xiang-Fei Li Chun-Nuan Zhang Chun-Nuan Zhang Wei-Hua Chen Wei-Hua Chen Kenneth Prudence Abasubong Kenneth Prudence Abasubong Guang-Zhen Jiang Guang-Zhen Jiang |
| author_sort | Kang Xiao |
| collection | DOAJ |
| description | Although cortisol is considered the main glucocorticoid involved in regulating stress in fish by elevating the serum glucose, the presence of serum corticosterone and whether its level can be used as an indicator for fish stress activation and reflect the degree of fish stress remains to be determined. To comprehensively and accurately evaluate the performance of two glucocorticoids after stress, in this study, seven of the most common stressors (ammonia nitrogen, high-temperature, acid, alkali, Aeromonas hydrophila, and salinity) in aquaculture were selected. The levels of serum corticosterone, cortisol, and glucose were detected within 48 h, and then the dynamic changes were analyzed. In addition, the kidneys were collected to detect the expression of corresponding proteins involved in corticosterone and cortisol synthesis (3 β-HSD and CYP17A1) for further exploration. From the results obtained, the content of serum corticosterone was about 100 times higher than that of cortisol, and it was found that corticosterone and cortisol showed different dynamic changes within 48 h among the seven kinds of stress. In the subsequent detection of glucose, it was found that the dynamic shift in glucose was not consistent with the dynamic changes of corticosterone or cortisol entirely but seemed to be caused by the combination of the two. In the follow-up protein expression detection, we found that corticosterone seemed to respond to stress earlier. Therefore, it is concluded that the responses of corticosterone and cortisol to stress are independent, corticosterone may be more sensitive in the current seven acute stresses, and then they regulate glucose together to resist stress. The study aimed to fill the vacancy of corticosterone in fish research and provide a reference for the complete evaluation of fish stress degree. |
| first_indexed | 2024-12-13T19:54:33Z |
| format | Article |
| id | doaj.art-f720914b556d476892f0d06c4690a93d |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-12-13T19:54:33Z |
| publishDate | 2021-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-f720914b556d476892f0d06c4690a93d2022-12-21T23:33:21ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452021-08-01810.3389/fmars.2021.692726692726Corticosterone Can Be an Essential Stress Index in Channel Catfish (Ictalurus punctatus)Kang Xiao0Kang Xiao1Xi Wang2Xi Wang3Wen-Bin Liu4Wen-Bin Liu5Ding-Dong Zhang6Ding-Dong Zhang7Xiang-Fei Li8Xiang-Fei Li9Chun-Nuan Zhang10Chun-Nuan Zhang11Wei-Hua Chen12Wei-Hua Chen13Kenneth Prudence Abasubong14Kenneth Prudence Abasubong15Guang-Zhen Jiang16Guang-Zhen Jiang17Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, ChinaNational Laboratory of Animal Science, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, ChinaNational Laboratory of Animal Science, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, ChinaNational Laboratory of Animal Science, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, ChinaNational Laboratory of Animal Science, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, ChinaNational Laboratory of Animal Science, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, ChinaNational Laboratory of Animal Science, Nanjing Agricultural University, Nanjing, ChinaNational Laboratory of Animal Science, Nanjing Agricultural University, Nanjing, ChinaCollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, ChinaNational Laboratory of Animal Science, Nanjing Agricultural University, Nanjing, ChinaKey Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, ChinaNational Laboratory of Animal Science, Nanjing Agricultural University, Nanjing, ChinaAlthough cortisol is considered the main glucocorticoid involved in regulating stress in fish by elevating the serum glucose, the presence of serum corticosterone and whether its level can be used as an indicator for fish stress activation and reflect the degree of fish stress remains to be determined. To comprehensively and accurately evaluate the performance of two glucocorticoids after stress, in this study, seven of the most common stressors (ammonia nitrogen, high-temperature, acid, alkali, Aeromonas hydrophila, and salinity) in aquaculture were selected. The levels of serum corticosterone, cortisol, and glucose were detected within 48 h, and then the dynamic changes were analyzed. In addition, the kidneys were collected to detect the expression of corresponding proteins involved in corticosterone and cortisol synthesis (3 β-HSD and CYP17A1) for further exploration. From the results obtained, the content of serum corticosterone was about 100 times higher than that of cortisol, and it was found that corticosterone and cortisol showed different dynamic changes within 48 h among the seven kinds of stress. In the subsequent detection of glucose, it was found that the dynamic shift in glucose was not consistent with the dynamic changes of corticosterone or cortisol entirely but seemed to be caused by the combination of the two. In the follow-up protein expression detection, we found that corticosterone seemed to respond to stress earlier. Therefore, it is concluded that the responses of corticosterone and cortisol to stress are independent, corticosterone may be more sensitive in the current seven acute stresses, and then they regulate glucose together to resist stress. The study aimed to fill the vacancy of corticosterone in fish research and provide a reference for the complete evaluation of fish stress degree.https://www.frontiersin.org/articles/10.3389/fmars.2021.692726/fullstresscortisolcorticosteroneCYP17A13β-HSDIctalurus punctatus |
| spellingShingle | Kang Xiao Kang Xiao Xi Wang Xi Wang Wen-Bin Liu Wen-Bin Liu Ding-Dong Zhang Ding-Dong Zhang Xiang-Fei Li Xiang-Fei Li Chun-Nuan Zhang Chun-Nuan Zhang Wei-Hua Chen Wei-Hua Chen Kenneth Prudence Abasubong Kenneth Prudence Abasubong Guang-Zhen Jiang Guang-Zhen Jiang Corticosterone Can Be an Essential Stress Index in Channel Catfish (Ictalurus punctatus) Frontiers in Marine Science stress cortisol corticosterone CYP17A1 3β-HSD Ictalurus punctatus |
| title | Corticosterone Can Be an Essential Stress Index in Channel Catfish (Ictalurus punctatus) |
| title_full | Corticosterone Can Be an Essential Stress Index in Channel Catfish (Ictalurus punctatus) |
| title_fullStr | Corticosterone Can Be an Essential Stress Index in Channel Catfish (Ictalurus punctatus) |
| title_full_unstemmed | Corticosterone Can Be an Essential Stress Index in Channel Catfish (Ictalurus punctatus) |
| title_short | Corticosterone Can Be an Essential Stress Index in Channel Catfish (Ictalurus punctatus) |
| title_sort | corticosterone can be an essential stress index in channel catfish ictalurus punctatus |
| topic | stress cortisol corticosterone CYP17A1 3β-HSD Ictalurus punctatus |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2021.692726/full |
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