Cytotoxicity Analysis for the Hydroxyl Functionalized MWCNT Reinforced PMMA Nanocomposites in Oral Squamous Carcinoma (KB) Cells
In this particular research study, a unique three-dimensional mixing technique was used to incorporate multi-walled carbon nanotubes (MWCNTs) into polymethyl methacrylate (PMMA), and the KB cell line was used in the analysis of cytotoxicity, apoptosis detection, and cell viability using the MTT assa...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/15/5/1192 |
| _version_ | 1797614554641858560 |
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| author | Vijay Patel Unnati Joshi Anand Joshi Tarun Kumar Upadhyay Lamya Ahmed Al-Keridis Mohd Saeed |
| author_facet | Vijay Patel Unnati Joshi Anand Joshi Tarun Kumar Upadhyay Lamya Ahmed Al-Keridis Mohd Saeed |
| author_sort | Vijay Patel |
| collection | DOAJ |
| description | In this particular research study, a unique three-dimensional mixing technique was used to incorporate multi-walled carbon nanotubes (MWCNTs) into polymethyl methacrylate (PMMA), and the KB cell line was used in the analysis of cytotoxicity, apoptosis detection, and cell viability using the MTT assay protocol. At low concentrations (0.001 to 0.1 g/mL), these results showed that the CNT did not seem to cause cell death or apoptosis directly. It increased lymphocyte-mediated cytotoxicity against KB cell lines. This was demonstrated by the fact that the CNT increased the time it took for KB cell lines to die. In the end, the unique three-dimensional mixing method solves problems such as clumping and uneven mixing that have been written about in the relevant literature. Phagocytic uptake of MWCNT-reinforced PMMA nanocomposite by KB cells leads to oxidative stress and apoptosis induction in a dose-dependent manner. The cytotoxicity of the generated composite and the ROS (reactive oxygen species) it produces may be controlled by adjusting the MWCNT loading. The conclusion that can be drawn from the studies to date is that it could be possible to treat some types of cancer using PMMA that has MWCNTs incorporated into it. |
| first_indexed | 2024-03-11T07:14:07Z |
| format | Article |
| id | doaj.art-f72207894d8f421f92c3330a6efc4f7a |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-11T07:14:07Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-f72207894d8f421f92c3330a6efc4f7a2023-11-17T08:27:24ZengMDPI AGPolymers2073-43602023-02-01155119210.3390/polym15051192Cytotoxicity Analysis for the Hydroxyl Functionalized MWCNT Reinforced PMMA Nanocomposites in Oral Squamous Carcinoma (KB) CellsVijay Patel0Unnati Joshi1Anand Joshi2Tarun Kumar Upadhyay3Lamya Ahmed Al-Keridis4Mohd Saeed5Department of Mechanical Engineering, Parul University, Vadodara 391760, Gujarat, IndiaDepartment of Mechanical Engineering, Parul University, Vadodara 391760, Gujarat, IndiaDepartment of Mechatronics Engineering, Parul University, Vadodara 391760, Gujarat, IndiaDepartment of Biotechnology, Parul Institute of Applied Sciences and Centre of Research for Development, Parul University, Vadodara 391760, Gujarat, IndiaBiology Department, Faculty of Science, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi ArabiaDepartment of Biology, College of Sciences, University of Hail, Hail 34464, Saudi ArabiaIn this particular research study, a unique three-dimensional mixing technique was used to incorporate multi-walled carbon nanotubes (MWCNTs) into polymethyl methacrylate (PMMA), and the KB cell line was used in the analysis of cytotoxicity, apoptosis detection, and cell viability using the MTT assay protocol. At low concentrations (0.001 to 0.1 g/mL), these results showed that the CNT did not seem to cause cell death or apoptosis directly. It increased lymphocyte-mediated cytotoxicity against KB cell lines. This was demonstrated by the fact that the CNT increased the time it took for KB cell lines to die. In the end, the unique three-dimensional mixing method solves problems such as clumping and uneven mixing that have been written about in the relevant literature. Phagocytic uptake of MWCNT-reinforced PMMA nanocomposite by KB cells leads to oxidative stress and apoptosis induction in a dose-dependent manner. The cytotoxicity of the generated composite and the ROS (reactive oxygen species) it produces may be controlled by adjusting the MWCNT loading. The conclusion that can be drawn from the studies to date is that it could be possible to treat some types of cancer using PMMA that has MWCNTs incorporated into it.https://www.mdpi.com/2073-4360/15/5/1192nanocompositecytotoxicitymultiwall carbon nanotubesPMMAKB cells |
| spellingShingle | Vijay Patel Unnati Joshi Anand Joshi Tarun Kumar Upadhyay Lamya Ahmed Al-Keridis Mohd Saeed Cytotoxicity Analysis for the Hydroxyl Functionalized MWCNT Reinforced PMMA Nanocomposites in Oral Squamous Carcinoma (KB) Cells Polymers nanocomposite cytotoxicity multiwall carbon nanotubes PMMA KB cells |
| title | Cytotoxicity Analysis for the Hydroxyl Functionalized MWCNT Reinforced PMMA Nanocomposites in Oral Squamous Carcinoma (KB) Cells |
| title_full | Cytotoxicity Analysis for the Hydroxyl Functionalized MWCNT Reinforced PMMA Nanocomposites in Oral Squamous Carcinoma (KB) Cells |
| title_fullStr | Cytotoxicity Analysis for the Hydroxyl Functionalized MWCNT Reinforced PMMA Nanocomposites in Oral Squamous Carcinoma (KB) Cells |
| title_full_unstemmed | Cytotoxicity Analysis for the Hydroxyl Functionalized MWCNT Reinforced PMMA Nanocomposites in Oral Squamous Carcinoma (KB) Cells |
| title_short | Cytotoxicity Analysis for the Hydroxyl Functionalized MWCNT Reinforced PMMA Nanocomposites in Oral Squamous Carcinoma (KB) Cells |
| title_sort | cytotoxicity analysis for the hydroxyl functionalized mwcnt reinforced pmma nanocomposites in oral squamous carcinoma kb cells |
| topic | nanocomposite cytotoxicity multiwall carbon nanotubes PMMA KB cells |
| url | https://www.mdpi.com/2073-4360/15/5/1192 |
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