Novel Vitronectin Variations and Their Comparative Analysis in Six Porcine Breeds
Vitronectin plays a role in the blood homeostasis and has been implicated in cell adhesion, migration, and proliferation. Vitronectin has a potential role affecting the residual feed intake (RFI) or feeding efficiency in swine production. Its variations have not been reported in Chinese swine breeds...
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MDPI AG
2019-08-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/9/8/520 |
| _version_ | 1819060100426366976 |
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| author | Wei Yan Xutin Zhao Juyin Li Long Cheng Yanqing Li |
| author_facet | Wei Yan Xutin Zhao Juyin Li Long Cheng Yanqing Li |
| author_sort | Wei Yan |
| collection | DOAJ |
| description | Vitronectin plays a role in the blood homeostasis and has been implicated in cell adhesion, migration, and proliferation. Vitronectin has a potential role affecting the residual feed intake (RFI) or feeding efficiency in swine production. Its variations have not been reported in Chinese swine breeds. In this study, two regions of porcine vitronectin were analyzed using PCR and sequencing. The sequence analysis revealed thirteen nucleotide substitutions in region 1 (exon 2- exon 3) and three nucleotide substitutions in region 2 (exon 5- intron 5), which would result in five amino acid changes (p.Ala52Thr, p.Leu94Pro, p.Leu94Gln, p.Gln94Pro, and p.Glu126Gly). In region 1, c.156C/T, c.281A/T, and c.377A/G were the most common (at a total frequency of 49.3%, 31.3% and 31.9% respectively), whereas c.153C/T and c.180C/G were rare (at a total frequency of 1.39%). In region 2, c.597 + 12A/G was the most common (at a total frequency of 39.6%), followed by c.597 + 15A/G (at a total frequency of 31.3%) and c.459A/G (at a total frequency of 16.0%). There was a difference (<i>p</i> < 0.05) in variant frequencies between Chinese breeds and overseas breeds. These results indicate that the porcine vitronectin gene is polymorphic and suggest further analysis is required to see if the variation detected affects RFI or feed efficiency in swines. |
| first_indexed | 2024-12-21T14:21:37Z |
| format | Article |
| id | doaj.art-f7224c855ca54c5c98612817c4b0301b |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-12-21T14:21:37Z |
| publishDate | 2019-08-01 |
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| spelling | doaj.art-f7224c855ca54c5c98612817c4b0301b2022-12-21T19:00:46ZengMDPI AGAnimals2076-26152019-08-019852010.3390/ani9080520ani9080520Novel Vitronectin Variations and Their Comparative Analysis in Six Porcine BreedsWei Yan0Xutin Zhao1Juyin Li2Long Cheng3Yanqing Li4School of Animal Science and Technology, Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, ChinaSchool of Animal Science and Technology, Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, ChinaSchool of Animal Science and Technology, Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, ChinaFaculty of Veterinary and Agricultural Sciences, The University of Melbourne, Dookie Campus, VIC 3647, AustraliaIPIG Management & Consulting Company Limited, Guangzhou 511457, ChinaVitronectin plays a role in the blood homeostasis and has been implicated in cell adhesion, migration, and proliferation. Vitronectin has a potential role affecting the residual feed intake (RFI) or feeding efficiency in swine production. Its variations have not been reported in Chinese swine breeds. In this study, two regions of porcine vitronectin were analyzed using PCR and sequencing. The sequence analysis revealed thirteen nucleotide substitutions in region 1 (exon 2- exon 3) and three nucleotide substitutions in region 2 (exon 5- intron 5), which would result in five amino acid changes (p.Ala52Thr, p.Leu94Pro, p.Leu94Gln, p.Gln94Pro, and p.Glu126Gly). In region 1, c.156C/T, c.281A/T, and c.377A/G were the most common (at a total frequency of 49.3%, 31.3% and 31.9% respectively), whereas c.153C/T and c.180C/G were rare (at a total frequency of 1.39%). In region 2, c.597 + 12A/G was the most common (at a total frequency of 39.6%), followed by c.597 + 15A/G (at a total frequency of 31.3%) and c.459A/G (at a total frequency of 16.0%). There was a difference (<i>p</i> < 0.05) in variant frequencies between Chinese breeds and overseas breeds. These results indicate that the porcine vitronectin gene is polymorphic and suggest further analysis is required to see if the variation detected affects RFI or feed efficiency in swines.https://www.mdpi.com/2076-2615/9/8/520Vitronectinfeed efficiencypigs |
| spellingShingle | Wei Yan Xutin Zhao Juyin Li Long Cheng Yanqing Li Novel Vitronectin Variations and Their Comparative Analysis in Six Porcine Breeds Animals Vitronectin feed efficiency pigs |
| title | Novel Vitronectin Variations and Their Comparative Analysis in Six Porcine Breeds |
| title_full | Novel Vitronectin Variations and Their Comparative Analysis in Six Porcine Breeds |
| title_fullStr | Novel Vitronectin Variations and Their Comparative Analysis in Six Porcine Breeds |
| title_full_unstemmed | Novel Vitronectin Variations and Their Comparative Analysis in Six Porcine Breeds |
| title_short | Novel Vitronectin Variations and Their Comparative Analysis in Six Porcine Breeds |
| title_sort | novel vitronectin variations and their comparative analysis in six porcine breeds |
| topic | Vitronectin feed efficiency pigs |
| url | https://www.mdpi.com/2076-2615/9/8/520 |
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