Valproic Acid Significantly Improves CRISPR/Cas9-Mediated Gene Editing
The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has emerged as a powerful technology, with the potential to generate transgenic animals. Particularly, efficient and precise genetic editing with CRISPR/Cas9 offers immense prospects in various biotechnological applic...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-06-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/9/6/1447 |
| _version_ | 1797565608584282112 |
|---|---|
| author | Hanseul Park Jaein Shin Hwan Choi Byounggook Cho Jongpil Kim |
| author_facet | Hanseul Park Jaein Shin Hwan Choi Byounggook Cho Jongpil Kim |
| author_sort | Hanseul Park |
| collection | DOAJ |
| description | The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has emerged as a powerful technology, with the potential to generate transgenic animals. Particularly, efficient and precise genetic editing with CRISPR/Cas9 offers immense prospects in various biotechnological applications. Here, we report that the histone deacetylase inhibitor valproic acid (VPA) significantly increases the efficiency of CRISPR/Cas9-mediated gene editing in mouse embryonic stem cells and embryos. This effect may be caused through globally enhanced chromatin accessibility, as indicate by histone hyperacetylation. Taken together, our results suggest that VPA can be used to increase the efficacy of CRISPR/Cas9 in generating transgenic systems. |
| first_indexed | 2024-03-10T19:15:34Z |
| format | Article |
| id | doaj.art-f723357dd86e45cfa7d43603a14ff2fd |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T19:15:34Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-f723357dd86e45cfa7d43603a14ff2fd2023-11-20T03:28:30ZengMDPI AGCells2073-44092020-06-0196144710.3390/cells9061447Valproic Acid Significantly Improves CRISPR/Cas9-Mediated Gene EditingHanseul Park0Jaein Shin1Hwan Choi2Byounggook Cho3Jongpil Kim4Department of Biomedical Engineering (BK21 Plus), Dongguk University, Seoul 04620, KoreaDepartment of Biomedical Engineering (BK21 Plus), Dongguk University, Seoul 04620, KoreaDepartment of Biomedical Engineering (BK21 Plus), Dongguk University, Seoul 04620, KoreaDepartment of Biomedical Engineering (BK21 Plus), Dongguk University, Seoul 04620, KoreaDepartment of Biomedical Engineering (BK21 Plus), Dongguk University, Seoul 04620, KoreaThe clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has emerged as a powerful technology, with the potential to generate transgenic animals. Particularly, efficient and precise genetic editing with CRISPR/Cas9 offers immense prospects in various biotechnological applications. Here, we report that the histone deacetylase inhibitor valproic acid (VPA) significantly increases the efficiency of CRISPR/Cas9-mediated gene editing in mouse embryonic stem cells and embryos. This effect may be caused through globally enhanced chromatin accessibility, as indicate by histone hyperacetylation. Taken together, our results suggest that VPA can be used to increase the efficacy of CRISPR/Cas9 in generating transgenic systems.https://www.mdpi.com/2073-4409/9/6/1447embryogene editingCRISPR/Cas9valproic acid |
| spellingShingle | Hanseul Park Jaein Shin Hwan Choi Byounggook Cho Jongpil Kim Valproic Acid Significantly Improves CRISPR/Cas9-Mediated Gene Editing Cells embryo gene editing CRISPR/Cas9 valproic acid |
| title | Valproic Acid Significantly Improves CRISPR/Cas9-Mediated Gene Editing |
| title_full | Valproic Acid Significantly Improves CRISPR/Cas9-Mediated Gene Editing |
| title_fullStr | Valproic Acid Significantly Improves CRISPR/Cas9-Mediated Gene Editing |
| title_full_unstemmed | Valproic Acid Significantly Improves CRISPR/Cas9-Mediated Gene Editing |
| title_short | Valproic Acid Significantly Improves CRISPR/Cas9-Mediated Gene Editing |
| title_sort | valproic acid significantly improves crispr cas9 mediated gene editing |
| topic | embryo gene editing CRISPR/Cas9 valproic acid |
| url | https://www.mdpi.com/2073-4409/9/6/1447 |
| work_keys_str_mv | AT hanseulpark valproicacidsignificantlyimprovescrisprcas9mediatedgeneediting AT jaeinshin valproicacidsignificantlyimprovescrisprcas9mediatedgeneediting AT hwanchoi valproicacidsignificantlyimprovescrisprcas9mediatedgeneediting AT byounggookcho valproicacidsignificantlyimprovescrisprcas9mediatedgeneediting AT jongpilkim valproicacidsignificantlyimprovescrisprcas9mediatedgeneediting |