Molecular epidemiological study of clinical cases of acute hepatitis E in Belarus
Relevance. The frequency of occurrence of anamnestic antibodies to the hepatitis E virus (HEV) in the general population of the Republic of Belarus is 7.3%, which is clearly not consistent with the low incidence of hepatitis E (HE). Most of primary HEV infections remain undiagnosed. The intensive ep...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | Russian |
| Published: |
Central Research Institute for Epidemiology
2023-01-01
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| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
| Subjects: | |
| Online Access: | https://microbiol.crie.ru/jour/article/viewFile/1355/787 |
| _version_ | 1811176864564117504 |
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| author | Vladimir V. Davydov Sergey V. Zhavoronok Tatyana V. Znovets Vladimir M. Tsyrkunov Andrei S. Babenkа Svetlana I. Marchuk Elena L. Gasich Tatiana P. Novik Galina I. Alatortseva Mikhail I. Mikhailov Vitaly V. Zverev |
| author_facet | Vladimir V. Davydov Sergey V. Zhavoronok Tatyana V. Znovets Vladimir M. Tsyrkunov Andrei S. Babenkа Svetlana I. Marchuk Elena L. Gasich Tatiana P. Novik Galina I. Alatortseva Mikhail I. Mikhailov Vitaly V. Zverev |
| author_sort | Vladimir V. Davydov |
| collection | DOAJ |
| description | Relevance. The frequency of occurrence of anamnestic antibodies to the hepatitis E virus (HEV) in the general population of the Republic of Belarus is 7.3%, which is clearly not consistent with the low incidence of hepatitis E (HE). Most of primary HEV infections remain undiagnosed. The intensive epidemic process of HEV in the Belarusian population is hidden. Conducting epidemiological studies, including genotyping of HEV sequences isolated on the territory of the republic, makes it possible to more accurately characterize the sources of HEV infection and the mechanisms of its transmission.
Aim molecular epidemiological study of two cases of acute hepatitis E detected in patients from Belarus.
Materials and methods. During 20212022, samples of biological material were obtained from two patients undergoing treatment with an established diagnosis of acute hepatitis E. Serum samples were tested to detect antibodies to HEV using enzyme immunoassay, HEV RNA was detected in fecal samples using nested RT-PCR. The nucleotide sequence was determined by an automatic sequencer using the Sanger method. Analysis of nucleotide sequences, their genotyping, and calculation of evolutionary distances were performed using MEGA X software.
Results. The HEV sequence isolated from a pregnant woman who had an epidemiological episode of alimentary contact with raw pork meat is clustered into a common phylogenetic clade with HEV sequence obtained from the patient from Belarus with a history of kidney transplantation and HEV sequences isolated from a domestic pigs. The HEV sequence isolated from a patient with a history of travel to Pakistan belongs to the HEV genotype 1 and joins a clade of HEV sequences isolated in Pakistan, India, Nepal and Mongolia. |
| first_indexed | 2024-04-10T19:59:51Z |
| format | Article |
| id | doaj.art-f72d38e7bbb6459ea11588bd8b0dd8a2 |
| institution | Directory Open Access Journal |
| issn | 0372-9311 2686-7613 |
| language | Russian |
| last_indexed | 2024-04-10T19:59:51Z |
| publishDate | 2023-01-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Журнал микробиологии, эпидемиологии и иммунобиологии |
| spelling | doaj.art-f72d38e7bbb6459ea11588bd8b0dd8a22023-01-27T12:56:03ZrusCentral Research Institute for EpidemiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132023-01-0199662563610.36233/0372-9311-328852Molecular epidemiological study of clinical cases of acute hepatitis E in BelarusVladimir V. Davydov0https://orcid.org/0000-0002-5672-9509Sergey V. Zhavoronok1https://orcid.org/0000-0001-9727-1103Tatyana V. Znovets2https://orcid.org/0000-0002-5603-490XVladimir M. Tsyrkunov3https://orcid.org/0000-0002-9366-6789Andrei S. Babenkа4https://orcid.org/0000-0002-5513-970XSvetlana I. Marchuk5https://orcid.org/0000-0002-2291-4538Elena L. Gasich6https://orcid.org/0000-0002-3662-3045Tatiana P. Novik7https://orcid.org/0000-0002-3220-9592Galina I. Alatortseva8https://orcid.org/0000-0001-9887-4061Mikhail I. Mikhailov9https://orcid.org/0000-0002-6636-6801Vitaly V. Zverev10https://orcid.org/0000-0002-0017-1892Belarusian State Medical UniversityBelarusian State Medical UniversityThird City Clinical Hospital named after E.V. KlumovGrodno State Medical UniversityBelarusian State Medical UniversityBelarusian State Medical UniversityRepublican Scientific and Practical Center of Epidemiology and MicrobiologyBelarusian State Medical UniversityI. Mechnikov Research Institute of Vaccines and SeraI. Mechnikov Research Institute of Vaccines and SeraI. Mechnikov Research Institute of Vaccines and SeraRelevance. The frequency of occurrence of anamnestic antibodies to the hepatitis E virus (HEV) in the general population of the Republic of Belarus is 7.3%, which is clearly not consistent with the low incidence of hepatitis E (HE). Most of primary HEV infections remain undiagnosed. The intensive epidemic process of HEV in the Belarusian population is hidden. Conducting epidemiological studies, including genotyping of HEV sequences isolated on the territory of the republic, makes it possible to more accurately characterize the sources of HEV infection and the mechanisms of its transmission. Aim molecular epidemiological study of two cases of acute hepatitis E detected in patients from Belarus. Materials and methods. During 20212022, samples of biological material were obtained from two patients undergoing treatment with an established diagnosis of acute hepatitis E. Serum samples were tested to detect antibodies to HEV using enzyme immunoassay, HEV RNA was detected in fecal samples using nested RT-PCR. The nucleotide sequence was determined by an automatic sequencer using the Sanger method. Analysis of nucleotide sequences, their genotyping, and calculation of evolutionary distances were performed using MEGA X software. Results. The HEV sequence isolated from a pregnant woman who had an epidemiological episode of alimentary contact with raw pork meat is clustered into a common phylogenetic clade with HEV sequence obtained from the patient from Belarus with a history of kidney transplantation and HEV sequences isolated from a domestic pigs. The HEV sequence isolated from a patient with a history of travel to Pakistan belongs to the HEV genotype 1 and joins a clade of HEV sequences isolated in Pakistan, India, Nepal and Mongolia.https://microbiol.crie.ru/jour/article/viewFile/1355/787hepatitis ephylogenetic analysisnucleotide sequences of hepatitis e virusmolecular epidemiology |
| spellingShingle | Vladimir V. Davydov Sergey V. Zhavoronok Tatyana V. Znovets Vladimir M. Tsyrkunov Andrei S. Babenkа Svetlana I. Marchuk Elena L. Gasich Tatiana P. Novik Galina I. Alatortseva Mikhail I. Mikhailov Vitaly V. Zverev Molecular epidemiological study of clinical cases of acute hepatitis E in Belarus Журнал микробиологии, эпидемиологии и иммунобиологии hepatitis e phylogenetic analysis nucleotide sequences of hepatitis e virus molecular epidemiology |
| title | Molecular epidemiological study of clinical cases of acute hepatitis E in Belarus |
| title_full | Molecular epidemiological study of clinical cases of acute hepatitis E in Belarus |
| title_fullStr | Molecular epidemiological study of clinical cases of acute hepatitis E in Belarus |
| title_full_unstemmed | Molecular epidemiological study of clinical cases of acute hepatitis E in Belarus |
| title_short | Molecular epidemiological study of clinical cases of acute hepatitis E in Belarus |
| title_sort | molecular epidemiological study of clinical cases of acute hepatitis e in belarus |
| topic | hepatitis e phylogenetic analysis nucleotide sequences of hepatitis e virus molecular epidemiology |
| url | https://microbiol.crie.ru/jour/article/viewFile/1355/787 |
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