An Improved Method of Optimizing the Extraction of Polyphenol Oxidase from Potato (Solanum tuberosum L.) Peel
The present study has an objective to optimize the extraction of Polyphenol Oxidase (PPO) from potato (Solanum tuberosum L.) peel. Response surface methodology (RSM) was used to design experiments and study the effect of six influential extraction parameters: extraction buffer concentration (100-500...
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| Format: | Article |
| Language: | English |
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Society of Land Measurements and Cadastre from Transylvania (SMTCT)
2012-02-01
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| Series: | Notulae Scientia Biologicae |
| Online Access: | http://www.notulaebiologicae.ro/index.php/nsb/article/view/7186 |
| _version_ | 1828751998887919616 |
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| author | Suprabhat MUKHERJEE Bidyut BANDYOPADHAYAY Bikram BASAK Nilrudra MANDAL Apurba DEY Biswanath MONDAL |
| author_facet | Suprabhat MUKHERJEE Bidyut BANDYOPADHAYAY Bikram BASAK Nilrudra MANDAL Apurba DEY Biswanath MONDAL |
| author_sort | Suprabhat MUKHERJEE |
| collection | DOAJ |
| description | The present study has an objective to optimize the extraction of Polyphenol Oxidase (PPO) from potato (Solanum tuberosum L.) peel. Response surface methodology (RSM) was used to design experiments and study the effect of six influential extraction parameters: extraction buffer concentration (100-500 mM), pH of extraction buffer (4.5-8.5), time (1-12 hours), temperature (4-40C), concentration of PMSF (1-5 mM) and volume of extraction buffer (200-1000 ml) on the extraction of PPO. The dependent variable was considered as response function which was specific activity (SA) of extracted PPO. ANOVA was performed to obtain the regression equation that could predict the responses within given range. From RSM generated model, the optimum conditions for the maximum extraction of PPO were phosphate buffer concentration of 100 mm, buffer pH of 4.5, extraction time of 1 hour, 40C temperature, PMSF concentration of 5 mM and buffer volume of 200 ml. Finally, this study illustrates a cost effective and less time consuming method to maximize the extraction of PPO from a vegetable waste. |
| first_indexed | 2024-12-10T21:02:48Z |
| format | Article |
| id | doaj.art-f72d97581b474b0499d737cebc160650 |
| institution | Directory Open Access Journal |
| issn | 2067-3205 2067-3264 |
| language | English |
| last_indexed | 2024-12-10T21:02:48Z |
| publishDate | 2012-02-01 |
| publisher | Society of Land Measurements and Cadastre from Transylvania (SMTCT) |
| record_format | Article |
| series | Notulae Scientia Biologicae |
| spelling | doaj.art-f72d97581b474b0499d737cebc1606502022-12-22T01:33:45ZengSociety of Land Measurements and Cadastre from Transylvania (SMTCT)Notulae Scientia Biologicae2067-32052067-32642012-02-01419810710.15835/nsb4171866914An Improved Method of Optimizing the Extraction of Polyphenol Oxidase from Potato (Solanum tuberosum L.) PeelSuprabhat MUKHERJEE0Bidyut BANDYOPADHAYAY1Bikram BASAK2Nilrudra MANDAL3Apurba DEY4Biswanath MONDAL5CSIR-Central Mechanical Engineering Research Institute, Centre for Advanced Materials Processing, 1Durgapur -713209Oriental Institute of Science and Technology, Department of Biotechnology, Burdwan3National Institute of Technology, Department of Biotechnology, Durgapur, Mahatma Gandhi Avenue, Durgapur-713209CSIR-Central Mechanical Engineering Research Institute, Centre for Advanced Materials Processing, 1Durgapur -7132093National Institute of Technology, Department of Biotechnology, Durgapur, Mahatma Gandhi Avenue, Durgapur-713209CSIR-Central Mechanical Engineering Research Institute, Centre for Advanced Materials Processing, 1Durgapur -713209The present study has an objective to optimize the extraction of Polyphenol Oxidase (PPO) from potato (Solanum tuberosum L.) peel. Response surface methodology (RSM) was used to design experiments and study the effect of six influential extraction parameters: extraction buffer concentration (100-500 mM), pH of extraction buffer (4.5-8.5), time (1-12 hours), temperature (4-40C), concentration of PMSF (1-5 mM) and volume of extraction buffer (200-1000 ml) on the extraction of PPO. The dependent variable was considered as response function which was specific activity (SA) of extracted PPO. ANOVA was performed to obtain the regression equation that could predict the responses within given range. From RSM generated model, the optimum conditions for the maximum extraction of PPO were phosphate buffer concentration of 100 mm, buffer pH of 4.5, extraction time of 1 hour, 40C temperature, PMSF concentration of 5 mM and buffer volume of 200 ml. Finally, this study illustrates a cost effective and less time consuming method to maximize the extraction of PPO from a vegetable waste.http://www.notulaebiologicae.ro/index.php/nsb/article/view/7186 |
| spellingShingle | Suprabhat MUKHERJEE Bidyut BANDYOPADHAYAY Bikram BASAK Nilrudra MANDAL Apurba DEY Biswanath MONDAL An Improved Method of Optimizing the Extraction of Polyphenol Oxidase from Potato (Solanum tuberosum L.) Peel Notulae Scientia Biologicae |
| title | An Improved Method of Optimizing the Extraction of Polyphenol Oxidase from Potato (Solanum tuberosum L.) Peel |
| title_full | An Improved Method of Optimizing the Extraction of Polyphenol Oxidase from Potato (Solanum tuberosum L.) Peel |
| title_fullStr | An Improved Method of Optimizing the Extraction of Polyphenol Oxidase from Potato (Solanum tuberosum L.) Peel |
| title_full_unstemmed | An Improved Method of Optimizing the Extraction of Polyphenol Oxidase from Potato (Solanum tuberosum L.) Peel |
| title_short | An Improved Method of Optimizing the Extraction of Polyphenol Oxidase from Potato (Solanum tuberosum L.) Peel |
| title_sort | improved method of optimizing the extraction of polyphenol oxidase from potato solanum tuberosum l peel |
| url | http://www.notulaebiologicae.ro/index.php/nsb/article/view/7186 |
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