Generation of a human induced pluripotent stem cell line (HMGUi002-A) from a healthy male individual
Induced pluripotent stem cells (iPSCs) can be used to generate different somatic cell types in vitro, including insulin-producing pancreatic β-cells. Here, we have generated iPSCs from a healthy male individual using an episomal reprogramming method. The resulting iPSCs are integration-free, have a...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019-08-01
|
| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506119301618 |
| _version_ | 1818173075149029376 |
|---|---|
| author | Xianming Wang Andrzej R. Malinowski Julia Beckenbauer Johanna Siehler Anna Blöchinger Thomas Meitinger Hans-Ulrich Häring Harald Staiger Ingo Burtscher Heiko Lickert |
| author_facet | Xianming Wang Andrzej R. Malinowski Julia Beckenbauer Johanna Siehler Anna Blöchinger Thomas Meitinger Hans-Ulrich Häring Harald Staiger Ingo Burtscher Heiko Lickert |
| author_sort | Xianming Wang |
| collection | DOAJ |
| description | Induced pluripotent stem cells (iPSCs) can be used to generate different somatic cell types in vitro, including insulin-producing pancreatic β-cells. Here, we have generated iPSCs from a healthy male individual using an episomal reprogramming method. The resulting iPSCs are integration-free, have a normal karyotype and are pluripotent in vitro and in vivo. Furthermore, we show that this iPSC line can be differentiated into pancreatic lineage cells. Taken together, this iPSC line will be useful to test differentiation protocols towards β-cell as well as other cell types and will also serve as a control for drug development and disease modelling studies. |
| first_indexed | 2024-12-11T19:22:44Z |
| format | Article |
| id | doaj.art-f72f05a930a24fe49056510d3ca4be9a |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-11T19:22:44Z |
| publishDate | 2019-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-f72f05a930a24fe49056510d3ca4be9a2022-12-22T00:53:29ZengElsevierStem Cell Research1873-50612019-08-0139Generation of a human induced pluripotent stem cell line (HMGUi002-A) from a healthy male individualXianming Wang0Andrzej R. Malinowski1Julia Beckenbauer2Johanna Siehler3Anna Blöchinger4Thomas Meitinger5Hans-Ulrich Häring6Harald Staiger7Ingo Burtscher8Heiko Lickert9Institute of Diabetes and Regeneration Research, Helmholtz Zentrum München, 85764 Neuherberg, Germany; Institute of Stem Cell Research, Helmholtz Zentrum München, 85764 Neuherberg, Germany; Technische Universität München, Ismaningerstraße 22, 81675 München, GermanyInstitute of Diabetes and Regeneration Research, Helmholtz Zentrum München, 85764 Neuherberg, Germany; Institute of Stem Cell Research, Helmholtz Zentrum München, 85764 Neuherberg, GermanyInstitute of Diabetes and Regeneration Research, Helmholtz Zentrum München, 85764 Neuherberg, Germany; Institute of Stem Cell Research, Helmholtz Zentrum München, 85764 Neuherberg, GermanyInstitute of Diabetes and Regeneration Research, Helmholtz Zentrum München, 85764 Neuherberg, Germany; Institute of Stem Cell Research, Helmholtz Zentrum München, 85764 Neuherberg, Germany; Technische Universität München, Ismaningerstraße 22, 81675 München, GermanyInstitute of Diabetes and Regeneration Research, Helmholtz Zentrum München, 85764 Neuherberg, Germany; Institute of Stem Cell Research, Helmholtz Zentrum München, 85764 Neuherberg, Germany; Technische Universität München, Ismaningerstraße 22, 81675 München, GermanyInstitute of Human Genetics, Helmholtz Zentrum München, Neuherberg, GermanyInstitute for Diabetes Research and Metabolic Diseases of the Helmholtz Zentrum München at the University of Tübingen, 72076 Tübingen, Germany; Department of Internal Medicine, Division of Endocrinology, Diabetology, Vascular Medicine, Nephrology and Clinical Chemistry, University of Tübingen, 72076 Tübingen, Germany; German Center for Diabetes Research (DZD), 85764 Neuherberg, GermanyInstitute for Diabetes Research and Metabolic Diseases of the Helmholtz Zentrum München at the University of Tübingen, 72076 Tübingen, Germany; Institute of Pharmaceutical Sciences, Department of Pharmacy and Biochemistry, Eberhard Karls University Tübingen, 72076 Tübingen, Germany; German Center for Diabetes Research (DZD), 85764 Neuherberg, GermanyInstitute of Diabetes and Regeneration Research, Helmholtz Zentrum München, 85764 Neuherberg, Germany; Institute of Stem Cell Research, Helmholtz Zentrum München, 85764 Neuherberg, GermanyInstitute of Diabetes and Regeneration Research, Helmholtz Zentrum München, 85764 Neuherberg, Germany; Institute of Stem Cell Research, Helmholtz Zentrum München, 85764 Neuherberg, Germany; Technische Universität München, Ismaningerstraße 22, 81675 München, Germany; German Center for Diabetes Research (DZD), 85764 Neuherberg, Germany; Corresponding author at: Helmholtz Zentrum München, Ingolstaedter Landstraße 1, 85764 Neuherberg, Germany.Induced pluripotent stem cells (iPSCs) can be used to generate different somatic cell types in vitro, including insulin-producing pancreatic β-cells. Here, we have generated iPSCs from a healthy male individual using an episomal reprogramming method. The resulting iPSCs are integration-free, have a normal karyotype and are pluripotent in vitro and in vivo. Furthermore, we show that this iPSC line can be differentiated into pancreatic lineage cells. Taken together, this iPSC line will be useful to test differentiation protocols towards β-cell as well as other cell types and will also serve as a control for drug development and disease modelling studies.http://www.sciencedirect.com/science/article/pii/S1873506119301618 |
| spellingShingle | Xianming Wang Andrzej R. Malinowski Julia Beckenbauer Johanna Siehler Anna Blöchinger Thomas Meitinger Hans-Ulrich Häring Harald Staiger Ingo Burtscher Heiko Lickert Generation of a human induced pluripotent stem cell line (HMGUi002-A) from a healthy male individual Stem Cell Research |
| title | Generation of a human induced pluripotent stem cell line (HMGUi002-A) from a healthy male individual |
| title_full | Generation of a human induced pluripotent stem cell line (HMGUi002-A) from a healthy male individual |
| title_fullStr | Generation of a human induced pluripotent stem cell line (HMGUi002-A) from a healthy male individual |
| title_full_unstemmed | Generation of a human induced pluripotent stem cell line (HMGUi002-A) from a healthy male individual |
| title_short | Generation of a human induced pluripotent stem cell line (HMGUi002-A) from a healthy male individual |
| title_sort | generation of a human induced pluripotent stem cell line hmgui002 a from a healthy male individual |
| url | http://www.sciencedirect.com/science/article/pii/S1873506119301618 |
| work_keys_str_mv | AT xianmingwang generationofahumaninducedpluripotentstemcelllinehmgui002afromahealthymaleindividual AT andrzejrmalinowski generationofahumaninducedpluripotentstemcelllinehmgui002afromahealthymaleindividual AT juliabeckenbauer generationofahumaninducedpluripotentstemcelllinehmgui002afromahealthymaleindividual AT johannasiehler generationofahumaninducedpluripotentstemcelllinehmgui002afromahealthymaleindividual AT annablochinger generationofahumaninducedpluripotentstemcelllinehmgui002afromahealthymaleindividual AT thomasmeitinger generationofahumaninducedpluripotentstemcelllinehmgui002afromahealthymaleindividual AT hansulrichharing generationofahumaninducedpluripotentstemcelllinehmgui002afromahealthymaleindividual AT haraldstaiger generationofahumaninducedpluripotentstemcelllinehmgui002afromahealthymaleindividual AT ingoburtscher generationofahumaninducedpluripotentstemcelllinehmgui002afromahealthymaleindividual AT heikolickert generationofahumaninducedpluripotentstemcelllinehmgui002afromahealthymaleindividual |