Anti-Ulcerative Colitis Effects and Active Ingredients in Ethyl Acetate Extract from Decoction of <i>Sargentodoxa cuneata</i>
Ulcerative colitis (UC) is an intractable disease prevalent worldwide. While ethyl acetate extract from decoction of <i>Sargentodoxa cuneata</i> (EAdSc) has potential anti-inflammatory activity, its effects on UC remain unknown. In this study, the constituent compounds discussed in the l...
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MDPI AG
2023-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/22/7663 |
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| author | Piao Yu Feng Xu Hongmei Wu Xiangpei Wang Qin Ding Mei Zhang Rongze Fang Ping Qin |
| author_facet | Piao Yu Feng Xu Hongmei Wu Xiangpei Wang Qin Ding Mei Zhang Rongze Fang Ping Qin |
| author_sort | Piao Yu |
| collection | DOAJ |
| description | Ulcerative colitis (UC) is an intractable disease prevalent worldwide. While ethyl acetate extract from decoction of <i>Sargentodoxa cuneata</i> (EAdSc) has potential anti-inflammatory activity, its effects on UC remain unknown. In this study, the constituent compounds discussed in the literature and identified by gas chromatography and mass spectrometry (GC–MS) were collected, and the blood-soluble components of EAdSc were identified by liquid chromatography–mass spectrometry. The network pharmacology analysis and molecular docking analysis were performed to explore the potential underlying mechanism and active ingredients of EAdSc against UC. Furthermore, mice with dextran sulfate sodium (DSS)-induced UC were used to study the therapeutic effects and validate the mechanism of EAdSc against UC. A total of 53 compounds from EAdSc were identified in the literature and by GC–MS, and 22 blood-soluble EAdSc components were recognized. Network pharmacology analysis revealed that multiple inflammatory signaling pathways are involved in EAdSc’s anti-UC activity. Furthermore, molecular docking analysis showed that the eleutheroside A, liriodendrin, epicatechin, 2-methoxy-4-vinylphenol, catechin, androsin, coumaroyltyramine, and catechol may be active against UC through the TLR4/NF-κB/NLRP3 pathway. EAdSc reduced the disease activity, macroscopic colon damage, and histological damage indices, as well as inhibiting DSS-induced spleen enlargement and colon shortening. In addition, EAdSc decreased the levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, and IL-17, as well as the expression of TLR4, NF-κB p65, NLRP3, and Caspase-1 mRNA in colon tissues. These results provide insights into the anti-UC effects and underlying mechanisms of EAdSc and help elucidate the active ingredients of EAdSc in the treatment of UC. |
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| last_indexed | 2024-03-09T16:34:50Z |
| publishDate | 2023-11-01 |
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| series | Molecules |
| spelling | doaj.art-f73141494ab949d89a80407503399cb32023-11-24T14:58:37ZengMDPI AGMolecules1420-30492023-11-012822766310.3390/molecules28227663Anti-Ulcerative Colitis Effects and Active Ingredients in Ethyl Acetate Extract from Decoction of <i>Sargentodoxa cuneata</i>Piao Yu0Feng Xu1Hongmei Wu2Xiangpei Wang3Qin Ding4Mei Zhang5Rongze Fang6Ping Qin7Department of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, ChinaDepartment of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, ChinaDepartment of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, ChinaSchool of Chinese Ethnic Medicine, Guizhou Minzu University, Guiyang 550025, ChinaDepartment of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, ChinaDepartment of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, ChinaDepartment of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, ChinaDepartment of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, ChinaUlcerative colitis (UC) is an intractable disease prevalent worldwide. While ethyl acetate extract from decoction of <i>Sargentodoxa cuneata</i> (EAdSc) has potential anti-inflammatory activity, its effects on UC remain unknown. In this study, the constituent compounds discussed in the literature and identified by gas chromatography and mass spectrometry (GC–MS) were collected, and the blood-soluble components of EAdSc were identified by liquid chromatography–mass spectrometry. The network pharmacology analysis and molecular docking analysis were performed to explore the potential underlying mechanism and active ingredients of EAdSc against UC. Furthermore, mice with dextran sulfate sodium (DSS)-induced UC were used to study the therapeutic effects and validate the mechanism of EAdSc against UC. A total of 53 compounds from EAdSc were identified in the literature and by GC–MS, and 22 blood-soluble EAdSc components were recognized. Network pharmacology analysis revealed that multiple inflammatory signaling pathways are involved in EAdSc’s anti-UC activity. Furthermore, molecular docking analysis showed that the eleutheroside A, liriodendrin, epicatechin, 2-methoxy-4-vinylphenol, catechin, androsin, coumaroyltyramine, and catechol may be active against UC through the TLR4/NF-κB/NLRP3 pathway. EAdSc reduced the disease activity, macroscopic colon damage, and histological damage indices, as well as inhibiting DSS-induced spleen enlargement and colon shortening. In addition, EAdSc decreased the levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, and IL-17, as well as the expression of TLR4, NF-κB p65, NLRP3, and Caspase-1 mRNA in colon tissues. These results provide insights into the anti-UC effects and underlying mechanisms of EAdSc and help elucidate the active ingredients of EAdSc in the treatment of UC.https://www.mdpi.com/1420-3049/28/22/7663<i>Sargentodoxa cuneata</i>ulcerative colitisgas chromatography and mass spectrometrymolecular dockingliquid chromatography–mass spectrometryTLR4/NF-κB/NLRP3 pathway |
| spellingShingle | Piao Yu Feng Xu Hongmei Wu Xiangpei Wang Qin Ding Mei Zhang Rongze Fang Ping Qin Anti-Ulcerative Colitis Effects and Active Ingredients in Ethyl Acetate Extract from Decoction of <i>Sargentodoxa cuneata</i> Molecules <i>Sargentodoxa cuneata</i> ulcerative colitis gas chromatography and mass spectrometry molecular docking liquid chromatography–mass spectrometry TLR4/NF-κB/NLRP3 pathway |
| title | Anti-Ulcerative Colitis Effects and Active Ingredients in Ethyl Acetate Extract from Decoction of <i>Sargentodoxa cuneata</i> |
| title_full | Anti-Ulcerative Colitis Effects and Active Ingredients in Ethyl Acetate Extract from Decoction of <i>Sargentodoxa cuneata</i> |
| title_fullStr | Anti-Ulcerative Colitis Effects and Active Ingredients in Ethyl Acetate Extract from Decoction of <i>Sargentodoxa cuneata</i> |
| title_full_unstemmed | Anti-Ulcerative Colitis Effects and Active Ingredients in Ethyl Acetate Extract from Decoction of <i>Sargentodoxa cuneata</i> |
| title_short | Anti-Ulcerative Colitis Effects and Active Ingredients in Ethyl Acetate Extract from Decoction of <i>Sargentodoxa cuneata</i> |
| title_sort | anti ulcerative colitis effects and active ingredients in ethyl acetate extract from decoction of i sargentodoxa cuneata i |
| topic | <i>Sargentodoxa cuneata</i> ulcerative colitis gas chromatography and mass spectrometry molecular docking liquid chromatography–mass spectrometry TLR4/NF-κB/NLRP3 pathway |
| url | https://www.mdpi.com/1420-3049/28/22/7663 |
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