First Description of a Temperate Bacteriophage (vB<i>_FhiM</i>_KIRK) of <i>Francisella hispaniensis</i> Strain 3523
Here we present the characterization of a <i>Francisella</i> bacteriophage (vB_<i>FhiM</i>_KIRK) including the morphology, the genome sequence and the induction of the prophage. The prophage sequence (FhaGI-1) has previously been identified in <i>F. hispaniensis</i&g...
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| Format: | Article |
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MDPI AG
2021-02-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/13/2/327 |
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| author | Kristin Köppen Grisna I. Prensa Kerstin Rydzewski Hana Tlapák Gudrun Holland Klaus Heuner |
| author_facet | Kristin Köppen Grisna I. Prensa Kerstin Rydzewski Hana Tlapák Gudrun Holland Klaus Heuner |
| author_sort | Kristin Köppen |
| collection | DOAJ |
| description | Here we present the characterization of a <i>Francisella</i> bacteriophage (vB_<i>FhiM</i>_KIRK) including the morphology, the genome sequence and the induction of the prophage. The prophage sequence (FhaGI-1) has previously been identified in <i>F. hispaniensis</i> strain 3523. UV radiation induced the prophage to assemble phage particles consisting of an icosahedral head (~52 nm in diameter), a tail of up to 97 nm in length and a mean width of 9 nm. The double stranded genome of vB_<i>FhiM</i>_KIRK contains 51 open reading frames and is 34,259 bp in length. The genotypic and phylogenetic analysis indicated that this phage seems to belong to the <i>Myoviridae</i> family of bacteriophages. Under the conditions tested here, host cell (<i>Francisella hispaniensis</i> 3523) lysis activity of KIRK was very low, and the phage particles seem to be defective for infecting new bacterial cells. Nevertheless, recombinant KIRK DNA was able to integrate site-specifically into the genome of different <i>Francisella</i> species after DNA transformation. |
| first_indexed | 2024-03-09T00:41:11Z |
| format | Article |
| id | doaj.art-f7319660746846e6904ee30531d74fe0 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T00:41:11Z |
| publishDate | 2021-02-01 |
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| series | Viruses |
| spelling | doaj.art-f7319660746846e6904ee30531d74fe02023-12-11T17:50:56ZengMDPI AGViruses1999-49152021-02-0113232710.3390/v13020327First Description of a Temperate Bacteriophage (vB<i>_FhiM</i>_KIRK) of <i>Francisella hispaniensis</i> Strain 3523Kristin Köppen0Grisna I. Prensa1Kerstin Rydzewski2Hana Tlapák3Gudrun Holland4Klaus Heuner5Centre for Biological Threats and Special Pathogens, Cellular Interactions of Bacterial Pathogens, ZBS 2, Robert Koch Institute, 13353 Berlin, GermanyCentre for Biological Threats and Special Pathogens, Cellular Interactions of Bacterial Pathogens, ZBS 2, Robert Koch Institute, 13353 Berlin, GermanyCentre for Biological Threats and Special Pathogens, Cellular Interactions of Bacterial Pathogens, ZBS 2, Robert Koch Institute, 13353 Berlin, GermanyCentre for Biological Threats and Special Pathogens, Cellular Interactions of Bacterial Pathogens, ZBS 2, Robert Koch Institute, 13353 Berlin, GermanyCentre for Biological Threats and Special Pathogens, Advanced Light and Electron Microscopy, ZBS 4, Robert Koch Institute, D-13353 Berlin, GermanyCentre for Biological Threats and Special Pathogens, Cellular Interactions of Bacterial Pathogens, ZBS 2, Robert Koch Institute, 13353 Berlin, GermanyHere we present the characterization of a <i>Francisella</i> bacteriophage (vB_<i>FhiM</i>_KIRK) including the morphology, the genome sequence and the induction of the prophage. The prophage sequence (FhaGI-1) has previously been identified in <i>F. hispaniensis</i> strain 3523. UV radiation induced the prophage to assemble phage particles consisting of an icosahedral head (~52 nm in diameter), a tail of up to 97 nm in length and a mean width of 9 nm. The double stranded genome of vB_<i>FhiM</i>_KIRK contains 51 open reading frames and is 34,259 bp in length. The genotypic and phylogenetic analysis indicated that this phage seems to belong to the <i>Myoviridae</i> family of bacteriophages. Under the conditions tested here, host cell (<i>Francisella hispaniensis</i> 3523) lysis activity of KIRK was very low, and the phage particles seem to be defective for infecting new bacterial cells. Nevertheless, recombinant KIRK DNA was able to integrate site-specifically into the genome of different <i>Francisella</i> species after DNA transformation.https://www.mdpi.com/1999-4915/13/2/327<i>Francisella hispaniensis</i>FhaGI-1prophageKIRKbacteriophage<i>Myoviridae</i> |
| spellingShingle | Kristin Köppen Grisna I. Prensa Kerstin Rydzewski Hana Tlapák Gudrun Holland Klaus Heuner First Description of a Temperate Bacteriophage (vB<i>_FhiM</i>_KIRK) of <i>Francisella hispaniensis</i> Strain 3523 Viruses <i>Francisella hispaniensis</i> FhaGI-1 prophage KIRK bacteriophage <i>Myoviridae</i> |
| title | First Description of a Temperate Bacteriophage (vB<i>_FhiM</i>_KIRK) of <i>Francisella hispaniensis</i> Strain 3523 |
| title_full | First Description of a Temperate Bacteriophage (vB<i>_FhiM</i>_KIRK) of <i>Francisella hispaniensis</i> Strain 3523 |
| title_fullStr | First Description of a Temperate Bacteriophage (vB<i>_FhiM</i>_KIRK) of <i>Francisella hispaniensis</i> Strain 3523 |
| title_full_unstemmed | First Description of a Temperate Bacteriophage (vB<i>_FhiM</i>_KIRK) of <i>Francisella hispaniensis</i> Strain 3523 |
| title_short | First Description of a Temperate Bacteriophage (vB<i>_FhiM</i>_KIRK) of <i>Francisella hispaniensis</i> Strain 3523 |
| title_sort | first description of a temperate bacteriophage vb i fhim i kirk of i francisella hispaniensis i strain 3523 |
| topic | <i>Francisella hispaniensis</i> FhaGI-1 prophage KIRK bacteriophage <i>Myoviridae</i> |
| url | https://www.mdpi.com/1999-4915/13/2/327 |
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