The Parasitome of the Phytonematode Heterodera glycines
Parasitism genes expressed in the esophageal gland cells of phytonematodes encode secretions that control the complex process of plant parasitism. In the soybean cyst nematode, Heterodera glycines, the parasitome, i.e., the secreted products of parasitism genes, facilitate nematode migration in soyb...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2003-08-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2003.16.8.720 |
| _version_ | 1818647125070708736 |
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| author | Bingli Gao R. Allen Tom Maier Eric L. Davis Thomas J. Baum Richard S. Hussey |
| author_facet | Bingli Gao R. Allen Tom Maier Eric L. Davis Thomas J. Baum Richard S. Hussey |
| author_sort | Bingli Gao |
| collection | DOAJ |
| description | Parasitism genes expressed in the esophageal gland cells of phytonematodes encode secretions that control the complex process of plant parasitism. In the soybean cyst nematode, Heterodera glycines, the parasitome, i.e., the secreted products of parasitism genes, facilitate nematode migration in soybean roots and mediate the modification of root cells into elaborate feeding cells required to support the growth and development of the nematode. With very few exceptions, the identities of these secretions are unknown, and the mechanisms of cyst nematode parasitism, therefore, remain obscure. The most direct and efficient approach for cloning parasitism genes and rapidly advancing our understanding of the molecular interactions during nematode parasitism of plants is to create gland cell-specific cDNA libraries using cytoplasm microaspirated from the esophageal gland cells of various parasitic stages. By combining expressed sequence tag analysis of a gland cell cDNA library with high throughput in situ expression localization of clones encoding secretory proteins, we obtained the first comprehensive parasitome profile for a parasitic nematode. We identified 51 new H. glycines gland-expressed candidate parasitism genes, of which 38 genes constitute completely novel sequences. Individual parasitome members showed distinct gland cell expression patterns throughout the parasitic cycle. The parasitome complexity discovered paints a more elaborate picture of host cellular events under specific control by the nematode parasite than previously hypothesized. |
| first_indexed | 2024-12-17T00:57:33Z |
| format | Article |
| id | doaj.art-f733cecd1bcc4d1daaa2547e4d711fa6 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-17T00:57:33Z |
| publishDate | 2003-08-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-f733cecd1bcc4d1daaa2547e4d711fa62022-12-21T22:09:34ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062003-08-0116872072610.1094/MPMI.2003.16.8.720The Parasitome of the Phytonematode Heterodera glycinesBingli GaoR. AllenTom MaierEric L. DavisThomas J. BaumRichard S. HusseyParasitism genes expressed in the esophageal gland cells of phytonematodes encode secretions that control the complex process of plant parasitism. In the soybean cyst nematode, Heterodera glycines, the parasitome, i.e., the secreted products of parasitism genes, facilitate nematode migration in soybean roots and mediate the modification of root cells into elaborate feeding cells required to support the growth and development of the nematode. With very few exceptions, the identities of these secretions are unknown, and the mechanisms of cyst nematode parasitism, therefore, remain obscure. The most direct and efficient approach for cloning parasitism genes and rapidly advancing our understanding of the molecular interactions during nematode parasitism of plants is to create gland cell-specific cDNA libraries using cytoplasm microaspirated from the esophageal gland cells of various parasitic stages. By combining expressed sequence tag analysis of a gland cell cDNA library with high throughput in situ expression localization of clones encoding secretory proteins, we obtained the first comprehensive parasitome profile for a parasitic nematode. We identified 51 new H. glycines gland-expressed candidate parasitism genes, of which 38 genes constitute completely novel sequences. Individual parasitome members showed distinct gland cell expression patterns throughout the parasitic cycle. The parasitome complexity discovered paints a more elaborate picture of host cellular events under specific control by the nematode parasite than previously hypothesized.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2003.16.8.720microaspirationplant-parasitic nematode |
| spellingShingle | Bingli Gao R. Allen Tom Maier Eric L. Davis Thomas J. Baum Richard S. Hussey The Parasitome of the Phytonematode Heterodera glycines Molecular Plant-Microbe Interactions microaspiration plant-parasitic nematode |
| title | The Parasitome of the Phytonematode Heterodera glycines |
| title_full | The Parasitome of the Phytonematode Heterodera glycines |
| title_fullStr | The Parasitome of the Phytonematode Heterodera glycines |
| title_full_unstemmed | The Parasitome of the Phytonematode Heterodera glycines |
| title_short | The Parasitome of the Phytonematode Heterodera glycines |
| title_sort | parasitome of the phytonematode heterodera glycines |
| topic | microaspiration plant-parasitic nematode |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2003.16.8.720 |
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