pH Regulates Endoglucanase Expression and Virulence of Alternaria alternata in Persimmon Fruit
The phytopathogenic fungus Alternaria alternata produces one endo-1,4-β-glucanase, AaK1, which is an important factor in disease development in persimmon fruit. During growth of A. alternata in media containing acidified yeast extract or cell walls from persimmon fruit, the fungus secreted ammonia a...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2002-08-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2002.15.8.774 |
| _version_ | 1818669149981769728 |
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| author | Dani Eshel Itay Miyara Tong Ailing Amos Dinoor Dov Prusky |
| author_facet | Dani Eshel Itay Miyara Tong Ailing Amos Dinoor Dov Prusky |
| author_sort | Dani Eshel |
| collection | DOAJ |
| description | The phytopathogenic fungus Alternaria alternata produces one endo-1,4-β-glucanase, AaK1, which is an important factor in disease development in persimmon fruit. During growth of A. alternata in media containing acidified yeast extract or cell walls from persimmon fruit, the fungus secreted ammonia and raised the medium pH. A rise in media pH from 3.8 to 6.0 in the presence of cell walls induced the expression of AaK1, whereas a glucose-induced decline in pH to 2.5 repressed transcription and enzymatic production. Treatments with buffered solutions at pH 6.0 during growth of A. alternata in the presence of glucose derepressed AaK1 expression and endo-1,4-β-glucanase production and enhanced decay development on the fruit. The results suggest that conditions affecting environmental pH modulate gene expression of AaK1 and virulence of A. alternata in persimmon fruit. |
| first_indexed | 2024-12-17T06:47:38Z |
| format | Article |
| id | doaj.art-f73844eee59d4203be6e85c06c1cb823 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-17T06:47:38Z |
| publishDate | 2002-08-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-f73844eee59d4203be6e85c06c1cb8232022-12-21T21:59:43ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062002-08-0115877477910.1094/MPMI.2002.15.8.774pH Regulates Endoglucanase Expression and Virulence of Alternaria alternata in Persimmon FruitDani EshelItay MiyaraTong AilingAmos DinoorDov PruskyThe phytopathogenic fungus Alternaria alternata produces one endo-1,4-β-glucanase, AaK1, which is an important factor in disease development in persimmon fruit. During growth of A. alternata in media containing acidified yeast extract or cell walls from persimmon fruit, the fungus secreted ammonia and raised the medium pH. A rise in media pH from 3.8 to 6.0 in the presence of cell walls induced the expression of AaK1, whereas a glucose-induced decline in pH to 2.5 repressed transcription and enzymatic production. Treatments with buffered solutions at pH 6.0 during growth of A. alternata in the presence of glucose derepressed AaK1 expression and endo-1,4-β-glucanase production and enhanced decay development on the fruit. The results suggest that conditions affecting environmental pH modulate gene expression of AaK1 and virulence of A. alternata in persimmon fruit.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2002.15.8.774postharvest diseases |
| spellingShingle | Dani Eshel Itay Miyara Tong Ailing Amos Dinoor Dov Prusky pH Regulates Endoglucanase Expression and Virulence of Alternaria alternata in Persimmon Fruit Molecular Plant-Microbe Interactions postharvest diseases |
| title | pH Regulates Endoglucanase Expression and Virulence of Alternaria alternata in Persimmon Fruit |
| title_full | pH Regulates Endoglucanase Expression and Virulence of Alternaria alternata in Persimmon Fruit |
| title_fullStr | pH Regulates Endoglucanase Expression and Virulence of Alternaria alternata in Persimmon Fruit |
| title_full_unstemmed | pH Regulates Endoglucanase Expression and Virulence of Alternaria alternata in Persimmon Fruit |
| title_short | pH Regulates Endoglucanase Expression and Virulence of Alternaria alternata in Persimmon Fruit |
| title_sort | ph regulates endoglucanase expression and virulence of alternaria alternata in persimmon fruit |
| topic | postharvest diseases |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2002.15.8.774 |
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