Protocol for the production and purification of an i-Motif-specific nanobody

Protocol for the production and purification of an i-Motif-specific nanobody

Summary: Intercalated motifs or i-Motifs (iMs) are nucleic acid structures formed by cytosine-rich sequences, which may regulate cellular processes and have broad applications in nanotechnology due to their pH-dependent nature. We have developed an iM-specific nanobody (iMbody) that can recognize iM...

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Bibliographic Details
Main Authors:	Mahdi Zeraati, Samuel E. Ross, Behnaz Aghaei, Alvaro González Rajal, Cecile King, Marcel E. Dinger
Format:	Article
Language:	English
Published:	Elsevier 2023-12-01
Series:	STAR Protocols
Subjects:	Genomics Microscopy Molecular Biology Antibody Molecular/Chemical Probes Protein Biochemistry
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166723006962

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