Water extract of Clinacanthus nutans leaves exhibits in vitro, ex vivo and in vivo anti-angiogenic activities in endothelial cell via suppression of cell proliferation
Abstract Background Clinacanthus nutans (Burm. f.) Lindau. has traditionally been using in South East Asia countries to manage cancer. However, scientific evidence is generally lacking to support this traditional claim. This study aims to investigate the in vitro, ex-vivo and in vivo effects of C. n...
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| Format: | Article |
| Language: | English |
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BMC
2018-07-01
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| Series: | BMC Complementary and Alternative Medicine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12906-018-2270-1 |
| _version_ | 1818114713441009664 |
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| author | Chin Theng Ng Lai Yen Fong Jun Jie Tan Nor Fadilah Rajab Faridah Abas Khozirah Shaari Kok Meng Chan Fariza Juliana Yoke Keong Yong |
| author_facet | Chin Theng Ng Lai Yen Fong Jun Jie Tan Nor Fadilah Rajab Faridah Abas Khozirah Shaari Kok Meng Chan Fariza Juliana Yoke Keong Yong |
| author_sort | Chin Theng Ng |
| collection | DOAJ |
| description | Abstract Background Clinacanthus nutans (Burm. f.) Lindau. has traditionally been using in South East Asia countries to manage cancer. However, scientific evidence is generally lacking to support this traditional claim. This study aims to investigate the in vitro, ex-vivo and in vivo effects of C. nutans extracts on angiogenesis. Methods C. nutans leaves was extracted with 50–100% ethanol or deionised water at 1% (w/v). Human umbilical veins endothelial cell (HUVEC) proliferation was examined using MTT assay. The in vitro anti-angiogenic effects of C. nutans were assessed using wound scratch, tube formation and transwell migration assays. The VEGF levels secreted by human oral squamous cell carcinoma (HSC-4) cell and HUVEC permeability were also measured. Besides, the rat aortic ring and chick embryo chorioallantoic membrane (CAM) assays, representing ex vivo and in vivo models, respectively, were performed. Results The MTT assay revealed that water extract of C. nutans leaves exhibited the highest activity, compared to the ethanol extracts. Therefore, the water extract was chosen for subsequent experiments. C. nutans leaf extract significantly suppressed endothelial cell proliferation and migration in both absence and presence of VEGF. However, the water extract failed to suppress HUVEC transmigration, differentiation and permeability. C. nutans water extract also did not suppress HSC-4 cell-induced VEGF production. Importantly, C. nutans water extract significantly abolished the sprouting of vessels in aortic rings as well as in chick embryo CAM. Conclusion In conclusion, these findings reveal potential anti-angiogenic effects of C. nutans, providing new evidence for its potential application as an anti-angiogenic agent. |
| first_indexed | 2024-12-11T03:55:06Z |
| format | Article |
| id | doaj.art-f73d8d13a16640da8ab18710089f5562 |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-11T03:55:06Z |
| publishDate | 2018-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-f73d8d13a16640da8ab18710089f55622022-12-22T01:21:48ZengBMCBMC Complementary and Alternative Medicine1472-68822018-07-0118111210.1186/s12906-018-2270-1Water extract of Clinacanthus nutans leaves exhibits in vitro, ex vivo and in vivo anti-angiogenic activities in endothelial cell via suppression of cell proliferationChin Theng Ng0Lai Yen Fong1Jun Jie Tan2Nor Fadilah Rajab3Faridah Abas4Khozirah Shaari5Kok Meng Chan6Fariza Juliana7Yoke Keong Yong8Physiology Unit, Faculty of Medicine, AIMST UniversityDepartment of Pre-clinical Sciences, Faculty of Medicine and Health Sciences, Universiti Tunku Abdul RahmanAdvance Medical and Dental Institute, Universiti Sains MalaysiaFaculty of Health Sciences, Universiti Kebangsaan MalaysiaDepartment of Food Science, Faculty of Food Science and Technology, Universiti Putra MalaysiaFaculty of Science, Universiti Putra MalaysiaFaculty of Health Sciences, Universiti Kebangsaan MalaysiaFaculty of Health Sciences, Universiti Kebangsaan MalaysiaDepartment of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra MalaysiaAbstract Background Clinacanthus nutans (Burm. f.) Lindau. has traditionally been using in South East Asia countries to manage cancer. However, scientific evidence is generally lacking to support this traditional claim. This study aims to investigate the in vitro, ex-vivo and in vivo effects of C. nutans extracts on angiogenesis. Methods C. nutans leaves was extracted with 50–100% ethanol or deionised water at 1% (w/v). Human umbilical veins endothelial cell (HUVEC) proliferation was examined using MTT assay. The in vitro anti-angiogenic effects of C. nutans were assessed using wound scratch, tube formation and transwell migration assays. The VEGF levels secreted by human oral squamous cell carcinoma (HSC-4) cell and HUVEC permeability were also measured. Besides, the rat aortic ring and chick embryo chorioallantoic membrane (CAM) assays, representing ex vivo and in vivo models, respectively, were performed. Results The MTT assay revealed that water extract of C. nutans leaves exhibited the highest activity, compared to the ethanol extracts. Therefore, the water extract was chosen for subsequent experiments. C. nutans leaf extract significantly suppressed endothelial cell proliferation and migration in both absence and presence of VEGF. However, the water extract failed to suppress HUVEC transmigration, differentiation and permeability. C. nutans water extract also did not suppress HSC-4 cell-induced VEGF production. Importantly, C. nutans water extract significantly abolished the sprouting of vessels in aortic rings as well as in chick embryo CAM. Conclusion In conclusion, these findings reveal potential anti-angiogenic effects of C. nutans, providing new evidence for its potential application as an anti-angiogenic agent.http://link.springer.com/article/10.1186/s12906-018-2270-1Clinacanthus nutansAcanthaceaeAngiogenesisChick embryo choriollantoic membraneAortic ring |
| spellingShingle | Chin Theng Ng Lai Yen Fong Jun Jie Tan Nor Fadilah Rajab Faridah Abas Khozirah Shaari Kok Meng Chan Fariza Juliana Yoke Keong Yong Water extract of Clinacanthus nutans leaves exhibits in vitro, ex vivo and in vivo anti-angiogenic activities in endothelial cell via suppression of cell proliferation BMC Complementary and Alternative Medicine Clinacanthus nutans Acanthaceae Angiogenesis Chick embryo choriollantoic membrane Aortic ring |
| title | Water extract of Clinacanthus nutans leaves exhibits in vitro, ex vivo and in vivo anti-angiogenic activities in endothelial cell via suppression of cell proliferation |
| title_full | Water extract of Clinacanthus nutans leaves exhibits in vitro, ex vivo and in vivo anti-angiogenic activities in endothelial cell via suppression of cell proliferation |
| title_fullStr | Water extract of Clinacanthus nutans leaves exhibits in vitro, ex vivo and in vivo anti-angiogenic activities in endothelial cell via suppression of cell proliferation |
| title_full_unstemmed | Water extract of Clinacanthus nutans leaves exhibits in vitro, ex vivo and in vivo anti-angiogenic activities in endothelial cell via suppression of cell proliferation |
| title_short | Water extract of Clinacanthus nutans leaves exhibits in vitro, ex vivo and in vivo anti-angiogenic activities in endothelial cell via suppression of cell proliferation |
| title_sort | water extract of clinacanthus nutans leaves exhibits in vitro ex vivo and in vivo anti angiogenic activities in endothelial cell via suppression of cell proliferation |
| topic | Clinacanthus nutans Acanthaceae Angiogenesis Chick embryo choriollantoic membrane Aortic ring |
| url | http://link.springer.com/article/10.1186/s12906-018-2270-1 |
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