Fast, large-field fluorescence and second-harmonic generation imaging with a single-spinning disk two-photon microscope

Confocal microscopes have been the workhorses of 3-D biological imaging, but they are slow, offer limited depth penetration and collect only ballistic photons. With their inefficient use of excitation photons they expose biological samples to an often intolerably high light burden. The speed limitat...

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Bibliographic Details
Main Authors: Deeg Andreas, Trigo Federico, Hazart Doriane, Delhomme Brigitte, Zar Tchyia, Naiser Thomas, Seebacher Christian, Salomon Adi, Ricard Clément, Uhl Rainer, Oheim Martin
Format: Article
Language:English
Published: EDP Sciences 2023-01-01
Series:EPJ Web of Conferences
Online Access:https://www.epj-conferences.org/articles/epjconf/pdf/2023/13/epjconf_eosam2023_03002.pdf