Effect of n-Butanol on Chromosomal Damage in Mice Bone Marrow Cells
<h2>Background: n-Butanol is a four-carbon alcohol used widely in foods, cosmetics industries, biology and chemistry research laboratories, and other fields. Long time-effects of inhalation or consumption of small amounts of Butanol on human health are still unknown. On the other hand, numerou...
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| Format: | Article |
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Shahid Beheshti University of Medical Sciences
2016-07-01
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| Series: | Novelty in Biomedicine |
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| Online Access: | http://journals.sbmu.ac.ir/index.php/nbm/article/view/10731 |
| _version_ | 1819096314848215040 |
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| author | Nahid Mansouri Farhang Haddad Masoud Fereidoni Bita Pourkaveh |
| author_facet | Nahid Mansouri Farhang Haddad Masoud Fereidoni Bita Pourkaveh |
| author_sort | Nahid Mansouri |
| collection | DOAJ |
| description | <h2>Background: n-Butanol is a four-carbon alcohol used widely in foods, cosmetics industries, biology and chemistry research laboratories, and other fields. Long time-effects of inhalation or consumption of small amounts of Butanol on human health are still unknown. On the other hand, numerous reports about the development of n-Butanol toxicity are available. The main objective of the study was to investigate the effects of inhaled and oral administration of n-Butanol as a long-term in vivo investigation.</h2><p><strong>M</strong><strong>aterials and Methods: </strong>Small white laboratory, male mice (20-30 g) were used in 11 groups (n=4) including experimental 1 to 6, 1 to 4 control "A” and positive control groups. Experimental groups 1-3, for 10, 20, and 40 days; 5 hours a day were inside a box with ventilation facilities exposed to air saturated with n-Butanol<strong> </strong>vapor. Experimental groups 4 to 6, received water containing n-Butanol<strong> </strong>0.2%, 1% and 5% for 10 days. Control groups B, 1 to 3 was placed for 10, 20, and 40 days inside a similar box exposed to normal air, respectively. Control group B 4 received water without any particular substance for 10 days. The positive control group received 30µl subcutaneous vinblastine. Bone marrow cells were extracted 24 hours after treatments and stained by May-Grünwald-Giemsa staining and the number of micronucleus was counted. Vinblastine, as a positive control, increased an average of micronucleus numbers significantly compared to control group (P<0.001).</p><p><strong>R</strong><strong>es</strong><strong>ults: </strong>n-Butanol<strong> </strong>inhalation caused no significant difference in 1-3 experimental groups in the average numbers of micronucleus compared to control group, even in the 40 days treatment group, average numbers of micronucleus was decreased comparing to control group (P<0.05). Also, oral administration of 0.2% and 1% n-Butanol<strong> </strong>had no effect on the average micronucleus numbers compared to the control group, while oral administration of 5% n-Butanol<strong> </strong>caused even decrease in average numbers of micronucleus compared to control group (P<0.05).</p><strong>Conclusion: </strong>n-Butanol<strong> </strong>inhalation may not cause chromosome damages in rat bone marrow cells that probably is due to its very fast metabolism and decomposition in the body. Therefore, the amount of n-Butanol<strong> </strong>in the systemic circulation and tissues is very low and, probably, the damaging potential is decreased. |
| first_indexed | 2024-12-21T23:57:14Z |
| format | Article |
| id | doaj.art-f741bbccf83142fd8fc89cdf9d2e0be4 |
| institution | Directory Open Access Journal |
| issn | 2345-3346 2345-3907 |
| language | English |
| last_indexed | 2024-12-21T23:57:14Z |
| publishDate | 2016-07-01 |
| publisher | Shahid Beheshti University of Medical Sciences |
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| series | Novelty in Biomedicine |
| spelling | doaj.art-f741bbccf83142fd8fc89cdf9d2e0be42022-12-21T18:45:47ZengShahid Beheshti University of Medical SciencesNovelty in Biomedicine2345-33462345-39072016-07-01431101157015Effect of n-Butanol on Chromosomal Damage in Mice Bone Marrow CellsNahid Mansouri0Farhang Haddad1Masoud Fereidoni2Bita Pourkaveh3Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, IranDepartment of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, IranDepartment of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, IranInfectious Diseases and Tropical Medicine Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran<h2>Background: n-Butanol is a four-carbon alcohol used widely in foods, cosmetics industries, biology and chemistry research laboratories, and other fields. Long time-effects of inhalation or consumption of small amounts of Butanol on human health are still unknown. On the other hand, numerous reports about the development of n-Butanol toxicity are available. The main objective of the study was to investigate the effects of inhaled and oral administration of n-Butanol as a long-term in vivo investigation.</h2><p><strong>M</strong><strong>aterials and Methods: </strong>Small white laboratory, male mice (20-30 g) were used in 11 groups (n=4) including experimental 1 to 6, 1 to 4 control "A” and positive control groups. Experimental groups 1-3, for 10, 20, and 40 days; 5 hours a day were inside a box with ventilation facilities exposed to air saturated with n-Butanol<strong> </strong>vapor. Experimental groups 4 to 6, received water containing n-Butanol<strong> </strong>0.2%, 1% and 5% for 10 days. Control groups B, 1 to 3 was placed for 10, 20, and 40 days inside a similar box exposed to normal air, respectively. Control group B 4 received water without any particular substance for 10 days. The positive control group received 30µl subcutaneous vinblastine. Bone marrow cells were extracted 24 hours after treatments and stained by May-Grünwald-Giemsa staining and the number of micronucleus was counted. Vinblastine, as a positive control, increased an average of micronucleus numbers significantly compared to control group (P<0.001).</p><p><strong>R</strong><strong>es</strong><strong>ults: </strong>n-Butanol<strong> </strong>inhalation caused no significant difference in 1-3 experimental groups in the average numbers of micronucleus compared to control group, even in the 40 days treatment group, average numbers of micronucleus was decreased comparing to control group (P<0.05). Also, oral administration of 0.2% and 1% n-Butanol<strong> </strong>had no effect on the average micronucleus numbers compared to the control group, while oral administration of 5% n-Butanol<strong> </strong>caused even decrease in average numbers of micronucleus compared to control group (P<0.05).</p><strong>Conclusion: </strong>n-Butanol<strong> </strong>inhalation may not cause chromosome damages in rat bone marrow cells that probably is due to its very fast metabolism and decomposition in the body. Therefore, the amount of n-Butanol<strong> </strong>in the systemic circulation and tissues is very low and, probably, the damaging potential is decreased.http://journals.sbmu.ac.ir/index.php/nbm/article/view/10731n-Butanol, Chromosomal damage, Bone marrow cells, Micronucleus |
| spellingShingle | Nahid Mansouri Farhang Haddad Masoud Fereidoni Bita Pourkaveh Effect of n-Butanol on Chromosomal Damage in Mice Bone Marrow Cells Novelty in Biomedicine n-Butanol, Chromosomal damage, Bone marrow cells, Micronucleus |
| title | Effect of n-Butanol on Chromosomal Damage in Mice Bone Marrow Cells |
| title_full | Effect of n-Butanol on Chromosomal Damage in Mice Bone Marrow Cells |
| title_fullStr | Effect of n-Butanol on Chromosomal Damage in Mice Bone Marrow Cells |
| title_full_unstemmed | Effect of n-Butanol on Chromosomal Damage in Mice Bone Marrow Cells |
| title_short | Effect of n-Butanol on Chromosomal Damage in Mice Bone Marrow Cells |
| title_sort | effect of n butanol on chromosomal damage in mice bone marrow cells |
| topic | n-Butanol, Chromosomal damage, Bone marrow cells, Micronucleus |
| url | http://journals.sbmu.ac.ir/index.php/nbm/article/view/10731 |
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