Evaluation of color based breast cancer cell images analysis

This paper describes a simple yet effective algorithm for automatically counting stained breast cancer cell imagesbased on color contents. The procedure for the approach consists of four steps. First, the cancer cell image in red-greenblue(RGB) color space is transformed with Haar wavelet. Second, t...

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Main Authors: Chalit Primkhajeepong, Pornchai Phukpattaranont, Somchai Limsiroratana, Pleumjit Boonyaphiphat, Kanita Kayasut3
Format: Article
Language:English
Published: Prince of Songkla University 2010-07-01
Series:Songklanakarin Journal of Science and Technology (SJST)
Subjects:
Online Access:http://www.rdoapp.psu.ac.th/html/sjst/journal/32-3/0125-3395-32-3-231-239.pdf
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author Chalit Primkhajeepong
Pornchai Phukpattaranont
Somchai Limsiroratana
Pleumjit Boonyaphiphat
Kanita Kayasut3
author_facet Chalit Primkhajeepong
Pornchai Phukpattaranont
Somchai Limsiroratana
Pleumjit Boonyaphiphat
Kanita Kayasut3
author_sort Chalit Primkhajeepong
collection DOAJ
description This paper describes a simple yet effective algorithm for automatically counting stained breast cancer cell imagesbased on color contents. The procedure for the approach consists of four steps. First, the cancer cell image in red-greenblue(RGB) color space is transformed with Haar wavelet. Second, the wavelet transformed image is changed to gray-scaleimage. Next, the gray-scale image is segmented with global thresholding using Otsu’s method and morphological operationsusing opening, region filling, border clearing, and watershed segmentation. Third, the wavelet transformed image is changedto CIEL*a*b* color space. The feature, i.e. average value of b* of each isolated cell, is extracted. Finally, the classification isapplied by using the extracted feature. If the average value of b* is positive that indicates yellow, the cancer cell is a positivecell. In addition, if the average value of b* is negative that indicates blue, the cancer cell is a negative cell. Results show thatthe classified cancer cells by the proposed algorithm are in good agreement with the expert perception. In addition, thealgorithm is practical to be used by a pathologist due to its simplicity and effectiveness.
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spelling doaj.art-f741c1837a284528beea3ccd7db9c3d12022-12-22T02:40:52ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952010-07-01323231239Evaluation of color based breast cancer cell images analysisChalit PrimkhajeepongPornchai PhukpattaranontSomchai LimsiroratanaPleumjit BoonyaphiphatKanita Kayasut3This paper describes a simple yet effective algorithm for automatically counting stained breast cancer cell imagesbased on color contents. The procedure for the approach consists of four steps. First, the cancer cell image in red-greenblue(RGB) color space is transformed with Haar wavelet. Second, the wavelet transformed image is changed to gray-scaleimage. Next, the gray-scale image is segmented with global thresholding using Otsu’s method and morphological operationsusing opening, region filling, border clearing, and watershed segmentation. Third, the wavelet transformed image is changedto CIEL*a*b* color space. The feature, i.e. average value of b* of each isolated cell, is extracted. Finally, the classification isapplied by using the extracted feature. If the average value of b* is positive that indicates yellow, the cancer cell is a positivecell. In addition, if the average value of b* is negative that indicates blue, the cancer cell is a negative cell. Results show thatthe classified cancer cells by the proposed algorithm are in good agreement with the expert perception. In addition, thealgorithm is practical to be used by a pathologist due to its simplicity and effectiveness.http://www.rdoapp.psu.ac.th/html/sjst/journal/32-3/0125-3395-32-3-231-239.pdfquantitative immunohistophathologybiomedical image processingimage segmentationwavelet transformationmorphological operation
spellingShingle Chalit Primkhajeepong
Pornchai Phukpattaranont
Somchai Limsiroratana
Pleumjit Boonyaphiphat
Kanita Kayasut3
Evaluation of color based breast cancer cell images analysis
Songklanakarin Journal of Science and Technology (SJST)
quantitative immunohistophathology
biomedical image processing
image segmentation
wavelet transformation
morphological operation
title Evaluation of color based breast cancer cell images analysis
title_full Evaluation of color based breast cancer cell images analysis
title_fullStr Evaluation of color based breast cancer cell images analysis
title_full_unstemmed Evaluation of color based breast cancer cell images analysis
title_short Evaluation of color based breast cancer cell images analysis
title_sort evaluation of color based breast cancer cell images analysis
topic quantitative immunohistophathology
biomedical image processing
image segmentation
wavelet transformation
morphological operation
url http://www.rdoapp.psu.ac.th/html/sjst/journal/32-3/0125-3395-32-3-231-239.pdf
work_keys_str_mv AT chalitprimkhajeepong evaluationofcolorbasedbreastcancercellimagesanalysis
AT pornchaiphukpattaranont evaluationofcolorbasedbreastcancercellimagesanalysis
AT somchailimsiroratana evaluationofcolorbasedbreastcancercellimagesanalysis
AT pleumjitboonyaphiphat evaluationofcolorbasedbreastcancercellimagesanalysis
AT kanitakayasut3 evaluationofcolorbasedbreastcancercellimagesanalysis