Transcriptome Analysis of the Marine Nematode <i>Litoditis marina</i> in a Chemically Defined Food Environment with Stearic Acid Supplementation
Stearic acid represents one of the most abundant fatty acids in the Western diet and profoundly regulates health and diseases of animals and human beings. We previously showed that stearic acid supplementation promoted development of the terrestrial model nematode <i>Caenorhabditis elegans<...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | Journal of Marine Science and Engineering |
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| Online Access: | https://www.mdpi.com/2077-1312/10/3/428 |
| _version_ | 1797470335664128000 |
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| author | Xuwen Cao Peiqi Sun Liusuo Zhang |
| author_facet | Xuwen Cao Peiqi Sun Liusuo Zhang |
| author_sort | Xuwen Cao |
| collection | DOAJ |
| description | Stearic acid represents one of the most abundant fatty acids in the Western diet and profoundly regulates health and diseases of animals and human beings. We previously showed that stearic acid supplementation promoted development of the terrestrial model nematode <i>Caenorhabditis elegans</i> in chemically defined CeMM food environment. However, whether stearic acid regulates development of other nematodes remains unknown. Here, we found that dietary supplementation with stearic acid could promote the development of the marine nematode <i>Litoditis marina</i>, belonging to the same family as <i>C. elegans</i>, indicating the conserved roles of stearic acid in developmental regulation. We further employed transcriptome analysis to analyze genome-wide transcriptional signatures of <i>L. marina</i> with dietary stearic acid supplementation. We found that stearic acid might promote development of <i>L. marina</i> via upregulation of the expression of genes involved in aminoacyl-tRNA biosynthesis, translation initiation and elongation, ribosome biogenesis, and transmembrane transport. In addition, we observed that the expression of neuronal signaling-related genes was decreased. This study provided important insights into how a single fatty acid stearic acid regulates development of marine nematode, and further studies with CRISPR genome editing will facilitate demonstrating the molecular mechanisms underlying how a single metabolite regulates animal development and health. |
| first_indexed | 2024-03-09T19:35:09Z |
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| id | doaj.art-f74240cc340b4d53a7cbe6844720141a |
| institution | Directory Open Access Journal |
| issn | 2077-1312 |
| language | English |
| last_indexed | 2024-03-09T19:35:09Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Marine Science and Engineering |
| spelling | doaj.art-f74240cc340b4d53a7cbe6844720141a2023-11-24T01:58:41ZengMDPI AGJournal of Marine Science and Engineering2077-13122022-03-0110342810.3390/jmse10030428Transcriptome Analysis of the Marine Nematode <i>Litoditis marina</i> in a Chemically Defined Food Environment with Stearic Acid SupplementationXuwen Cao0Peiqi Sun1Liusuo Zhang2CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaCAS and Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaCAS and Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaStearic acid represents one of the most abundant fatty acids in the Western diet and profoundly regulates health and diseases of animals and human beings. We previously showed that stearic acid supplementation promoted development of the terrestrial model nematode <i>Caenorhabditis elegans</i> in chemically defined CeMM food environment. However, whether stearic acid regulates development of other nematodes remains unknown. Here, we found that dietary supplementation with stearic acid could promote the development of the marine nematode <i>Litoditis marina</i>, belonging to the same family as <i>C. elegans</i>, indicating the conserved roles of stearic acid in developmental regulation. We further employed transcriptome analysis to analyze genome-wide transcriptional signatures of <i>L. marina</i> with dietary stearic acid supplementation. We found that stearic acid might promote development of <i>L. marina</i> via upregulation of the expression of genes involved in aminoacyl-tRNA biosynthesis, translation initiation and elongation, ribosome biogenesis, and transmembrane transport. In addition, we observed that the expression of neuronal signaling-related genes was decreased. This study provided important insights into how a single fatty acid stearic acid regulates development of marine nematode, and further studies with CRISPR genome editing will facilitate demonstrating the molecular mechanisms underlying how a single metabolite regulates animal development and health.https://www.mdpi.com/2077-1312/10/3/428marine nematodestearic acidCeMMdevelopmenttranscriptomeprotein translation |
| spellingShingle | Xuwen Cao Peiqi Sun Liusuo Zhang Transcriptome Analysis of the Marine Nematode <i>Litoditis marina</i> in a Chemically Defined Food Environment with Stearic Acid Supplementation Journal of Marine Science and Engineering marine nematode stearic acid CeMM development transcriptome protein translation |
| title | Transcriptome Analysis of the Marine Nematode <i>Litoditis marina</i> in a Chemically Defined Food Environment with Stearic Acid Supplementation |
| title_full | Transcriptome Analysis of the Marine Nematode <i>Litoditis marina</i> in a Chemically Defined Food Environment with Stearic Acid Supplementation |
| title_fullStr | Transcriptome Analysis of the Marine Nematode <i>Litoditis marina</i> in a Chemically Defined Food Environment with Stearic Acid Supplementation |
| title_full_unstemmed | Transcriptome Analysis of the Marine Nematode <i>Litoditis marina</i> in a Chemically Defined Food Environment with Stearic Acid Supplementation |
| title_short | Transcriptome Analysis of the Marine Nematode <i>Litoditis marina</i> in a Chemically Defined Food Environment with Stearic Acid Supplementation |
| title_sort | transcriptome analysis of the marine nematode i litoditis marina i in a chemically defined food environment with stearic acid supplementation |
| topic | marine nematode stearic acid CeMM development transcriptome protein translation |
| url | https://www.mdpi.com/2077-1312/10/3/428 |
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