Regionally specific levels and patterns of keratin 8 expression in the mouse embryo visceral endoderm emerge upon anterior-posterior axis determination
The mechanical properties of the different germ layers of the early mammalian embryo are likely to be critical for morphogenesis. Cytoskeleton components (actin and myosin, microtubules, intermediate filaments) are major determinants of epithelial plasticity and resilience to stress. Here, we take a...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2022.1037041/full |
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| author | Evangéline Despin-Guitard Ronan Quenec’Hdu Wallis Nahaboo Nicole Schwarz Rudolf E. Leube Claire Chazaud Isabelle Migeotte |
| author_facet | Evangéline Despin-Guitard Ronan Quenec’Hdu Wallis Nahaboo Nicole Schwarz Rudolf E. Leube Claire Chazaud Isabelle Migeotte |
| author_sort | Evangéline Despin-Guitard |
| collection | DOAJ |
| description | The mechanical properties of the different germ layers of the early mammalian embryo are likely to be critical for morphogenesis. Cytoskeleton components (actin and myosin, microtubules, intermediate filaments) are major determinants of epithelial plasticity and resilience to stress. Here, we take advantage of a mouse reporter for Keratin 8 to record the pattern of the keratin intermediate filaments network in the first epithelia of the developing mouse embryo. At the blastocyst stage, Keratin 8 is strongly expressed in the trophectoderm, and undetectable in the inner cell mass and its derivatives, the epiblast and primitive endoderm. Visceral endoderm cells that differentiate from the primitive endoderm at the egg cylinder stage display apical Keratin 8 filaments. Upon migration of the Anterior Visceral Endoderm and determination of the anterior-posterior axis, Keratin 8 becomes regionally distributed, with a stronger expression in embryonic, compared to extra-embryonic, visceral endoderm. This pattern emerges concomitantly to a modification of the distribution of Filamentous (F)-actin, from a cortical ring to a dense apical shroud, in extra-embryonic visceral endoderm only. Those regional characteristics are maintained across gastrulation. Interestingly, for each stage and region of the embryo, adjacent germ layers display contrasted levels of keratin filaments, which may play a role in their adaptation to growth and morphological changes. |
| first_indexed | 2024-04-11T07:57:37Z |
| format | Article |
| id | doaj.art-f746ed3927ff46ad8e4a9bfc52eda0f3 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-04-11T07:57:37Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-f746ed3927ff46ad8e4a9bfc52eda0f32022-12-22T04:35:54ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2022-12-011010.3389/fcell.2022.10370411037041Regionally specific levels and patterns of keratin 8 expression in the mouse embryo visceral endoderm emerge upon anterior-posterior axis determinationEvangéline Despin-Guitard0Ronan Quenec’Hdu1Wallis Nahaboo2Nicole Schwarz3Rudolf E. Leube4Claire Chazaud5Isabelle Migeotte6Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire (IRIBHM), Université Libre de Bruxelles, Brussels, BelgiumUniversité Clermont Auvergne, CNRS, INSERM, GReD Institute, Faculté de Médecine, Clermont-Ferrand, FranceInstitut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire (IRIBHM), Université Libre de Bruxelles, Brussels, BelgiumInstitute of Molecular and Cellular Anatomy, RWTH Aachen University, Aachen, GermanyInstitute of Molecular and Cellular Anatomy, RWTH Aachen University, Aachen, GermanyUniversité Clermont Auvergne, CNRS, INSERM, GReD Institute, Faculté de Médecine, Clermont-Ferrand, FranceInstitut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire (IRIBHM), Université Libre de Bruxelles, Brussels, BelgiumThe mechanical properties of the different germ layers of the early mammalian embryo are likely to be critical for morphogenesis. Cytoskeleton components (actin and myosin, microtubules, intermediate filaments) are major determinants of epithelial plasticity and resilience to stress. Here, we take advantage of a mouse reporter for Keratin 8 to record the pattern of the keratin intermediate filaments network in the first epithelia of the developing mouse embryo. At the blastocyst stage, Keratin 8 is strongly expressed in the trophectoderm, and undetectable in the inner cell mass and its derivatives, the epiblast and primitive endoderm. Visceral endoderm cells that differentiate from the primitive endoderm at the egg cylinder stage display apical Keratin 8 filaments. Upon migration of the Anterior Visceral Endoderm and determination of the anterior-posterior axis, Keratin 8 becomes regionally distributed, with a stronger expression in embryonic, compared to extra-embryonic, visceral endoderm. This pattern emerges concomitantly to a modification of the distribution of Filamentous (F)-actin, from a cortical ring to a dense apical shroud, in extra-embryonic visceral endoderm only. Those regional characteristics are maintained across gastrulation. Interestingly, for each stage and region of the embryo, adjacent germ layers display contrasted levels of keratin filaments, which may play a role in their adaptation to growth and morphological changes.https://www.frontiersin.org/articles/10.3389/fcell.2022.1037041/fullmouse embryo developmentextra-embryonic tissueskeratin intermediate filamentsepitheliacytoskeleton |
| spellingShingle | Evangéline Despin-Guitard Ronan Quenec’Hdu Wallis Nahaboo Nicole Schwarz Rudolf E. Leube Claire Chazaud Isabelle Migeotte Regionally specific levels and patterns of keratin 8 expression in the mouse embryo visceral endoderm emerge upon anterior-posterior axis determination Frontiers in Cell and Developmental Biology mouse embryo development extra-embryonic tissues keratin intermediate filaments epithelia cytoskeleton |
| title | Regionally specific levels and patterns of keratin 8 expression in the mouse embryo visceral endoderm emerge upon anterior-posterior axis determination |
| title_full | Regionally specific levels and patterns of keratin 8 expression in the mouse embryo visceral endoderm emerge upon anterior-posterior axis determination |
| title_fullStr | Regionally specific levels and patterns of keratin 8 expression in the mouse embryo visceral endoderm emerge upon anterior-posterior axis determination |
| title_full_unstemmed | Regionally specific levels and patterns of keratin 8 expression in the mouse embryo visceral endoderm emerge upon anterior-posterior axis determination |
| title_short | Regionally specific levels and patterns of keratin 8 expression in the mouse embryo visceral endoderm emerge upon anterior-posterior axis determination |
| title_sort | regionally specific levels and patterns of keratin 8 expression in the mouse embryo visceral endoderm emerge upon anterior posterior axis determination |
| topic | mouse embryo development extra-embryonic tissues keratin intermediate filaments epithelia cytoskeleton |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2022.1037041/full |
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