A Small-RNA-Mediated Feedback Loop Maintains Proper Levels of 22G-RNAs in C. elegans
Summary: RNA interference (RNAi) is an essential regulatory mechanism in all animals. In Caenorhabditis elegans, several classes of small RNAs act to silence or license expression of mRNA targets. ERI-6/7 is required for the production of some endogenous small interfering RNAs (siRNAs) and acts as a...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-10-01
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| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124720312687 |
| _version_ | 1818242154750803968 |
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| author | Alicia K. Rogers Carolyn M. Phillips |
| author_facet | Alicia K. Rogers Carolyn M. Phillips |
| author_sort | Alicia K. Rogers |
| collection | DOAJ |
| description | Summary: RNA interference (RNAi) is an essential regulatory mechanism in all animals. In Caenorhabditis elegans, several classes of small RNAs act to silence or license expression of mRNA targets. ERI-6/7 is required for the production of some endogenous small interfering RNAs (siRNAs) and acts as a negative regulator of the exogenous RNAi pathway. We find that the genomic locus encoding eri-6/7 contains two distinct regions that are targeted by endogenous siRNAs. Loss of these siRNAs disrupts eri-6/7 mRNA expression, resulting in increased production of siRNAs from other small RNA pathways because these pathways compete with eri-6/7-dependent transcripts for access to the downstream siRNA amplification machinery. Thus, the pathway acts like a small-RNA-mediated feedback loop to ensure homeostasis of gene expression by small RNA pathways. Similar feedback loops that maintain chromatin homeostasis have been identified in yeast and Drosophila melanogaster, suggesting an evolutionary conservation of feedback mechanisms in gene regulatory pathways. |
| first_indexed | 2024-12-12T13:40:43Z |
| format | Article |
| id | doaj.art-f74bafc2fd2c47019734710e953a049d |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-12T13:40:43Z |
| publishDate | 2020-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-f74bafc2fd2c47019734710e953a049d2022-12-22T00:22:48ZengElsevierCell Reports2211-12472020-10-01333108279A Small-RNA-Mediated Feedback Loop Maintains Proper Levels of 22G-RNAs in C. elegansAlicia K. Rogers0Carolyn M. Phillips1Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089, USADepartment of Biological Sciences, University of Southern California, Los Angeles, CA 90089, USA; Corresponding authorSummary: RNA interference (RNAi) is an essential regulatory mechanism in all animals. In Caenorhabditis elegans, several classes of small RNAs act to silence or license expression of mRNA targets. ERI-6/7 is required for the production of some endogenous small interfering RNAs (siRNAs) and acts as a negative regulator of the exogenous RNAi pathway. We find that the genomic locus encoding eri-6/7 contains two distinct regions that are targeted by endogenous siRNAs. Loss of these siRNAs disrupts eri-6/7 mRNA expression, resulting in increased production of siRNAs from other small RNA pathways because these pathways compete with eri-6/7-dependent transcripts for access to the downstream siRNA amplification machinery. Thus, the pathway acts like a small-RNA-mediated feedback loop to ensure homeostasis of gene expression by small RNA pathways. Similar feedback loops that maintain chromatin homeostasis have been identified in yeast and Drosophila melanogaster, suggesting an evolutionary conservation of feedback mechanisms in gene regulatory pathways.http://www.sciencedirect.com/science/article/pii/S2211124720312687RNAiC. eleganssmall RNAgene regulationfeedback loop22G-RNAs |
| spellingShingle | Alicia K. Rogers Carolyn M. Phillips A Small-RNA-Mediated Feedback Loop Maintains Proper Levels of 22G-RNAs in C. elegans Cell Reports RNAi C. elegans small RNA gene regulation feedback loop 22G-RNAs |
| title | A Small-RNA-Mediated Feedback Loop Maintains Proper Levels of 22G-RNAs in C. elegans |
| title_full | A Small-RNA-Mediated Feedback Loop Maintains Proper Levels of 22G-RNAs in C. elegans |
| title_fullStr | A Small-RNA-Mediated Feedback Loop Maintains Proper Levels of 22G-RNAs in C. elegans |
| title_full_unstemmed | A Small-RNA-Mediated Feedback Loop Maintains Proper Levels of 22G-RNAs in C. elegans |
| title_short | A Small-RNA-Mediated Feedback Loop Maintains Proper Levels of 22G-RNAs in C. elegans |
| title_sort | small rna mediated feedback loop maintains proper levels of 22g rnas in c elegans |
| topic | RNAi C. elegans small RNA gene regulation feedback loop 22G-RNAs |
| url | http://www.sciencedirect.com/science/article/pii/S2211124720312687 |
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