Pseudomonas aeruginosa reference strains PAO1 and PA14: A genomic, phenotypic, and therapeutic review
Pseudomonas aeruginosa is a ubiquitous, motile, gram-negative bacterium that has been recently identified as a multi-drug resistant pathogen in critical need of novel therapeutics. Of the approximately 5,000 strains, PAO1 and PA14 are common laboratory reference strains, modeling moderately and hype...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1023523/full |
| _version_ | 1811241031612497920 |
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| author | Amber Grace Rajnish Sahu Donald R. Owen Vida A. Dennis |
| author_facet | Amber Grace Rajnish Sahu Donald R. Owen Vida A. Dennis |
| author_sort | Amber Grace |
| collection | DOAJ |
| description | Pseudomonas aeruginosa is a ubiquitous, motile, gram-negative bacterium that has been recently identified as a multi-drug resistant pathogen in critical need of novel therapeutics. Of the approximately 5,000 strains, PAO1 and PA14 are common laboratory reference strains, modeling moderately and hyper-virulent phenotypes, respectively. PAO1 and PA14 have been instrumental in facilitating the discovery of novel drug targets, testing novel therapeutics, and supplying critical genomic information on the bacterium. While the two strains have contributed to a wide breadth of knowledge on the natural behaviors and therapeutic susceptibilities of P. aeruginosa, they have demonstrated significant deviations from observations in human infections. Many of these deviations are related to experimental inconsistencies in laboratory strain environment that complicate and, at times, terminate translation from laboratory results to clinical applications. This review aims to provide a comparative analysis of the two strains and potential methods to improve their clinical relevance. |
| first_indexed | 2024-04-12T13:29:40Z |
| format | Article |
| id | doaj.art-f74bd5be9ec04bb39e94a0a30a3b0b8e |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T13:29:40Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-f74bd5be9ec04bb39e94a0a30a3b0b8e2022-12-22T03:31:13ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-10-011310.3389/fmicb.2022.10235231023523Pseudomonas aeruginosa reference strains PAO1 and PA14: A genomic, phenotypic, and therapeutic reviewAmber Grace0Rajnish Sahu1Donald R. Owen2Vida A. Dennis3Department of Biological Sciences, Alabama State University, Montgomery, AL, United StatesDepartment of Biological Sciences, Alabama State University, Montgomery, AL, United StatesOwen Biosciences Inc., Baton Rouge, LA, United StatesDepartment of Biological Sciences, Alabama State University, Montgomery, AL, United StatesPseudomonas aeruginosa is a ubiquitous, motile, gram-negative bacterium that has been recently identified as a multi-drug resistant pathogen in critical need of novel therapeutics. Of the approximately 5,000 strains, PAO1 and PA14 are common laboratory reference strains, modeling moderately and hyper-virulent phenotypes, respectively. PAO1 and PA14 have been instrumental in facilitating the discovery of novel drug targets, testing novel therapeutics, and supplying critical genomic information on the bacterium. While the two strains have contributed to a wide breadth of knowledge on the natural behaviors and therapeutic susceptibilities of P. aeruginosa, they have demonstrated significant deviations from observations in human infections. Many of these deviations are related to experimental inconsistencies in laboratory strain environment that complicate and, at times, terminate translation from laboratory results to clinical applications. This review aims to provide a comparative analysis of the two strains and potential methods to improve their clinical relevance.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1023523/fullPseudomonas aeruginosaPAO1PA14Pseudomonas genomeESKAPE pathogensclinical relevance of Pseudomonas aeruginosa strains |
| spellingShingle | Amber Grace Rajnish Sahu Donald R. Owen Vida A. Dennis Pseudomonas aeruginosa reference strains PAO1 and PA14: A genomic, phenotypic, and therapeutic review Frontiers in Microbiology Pseudomonas aeruginosa PAO1 PA14 Pseudomonas genome ESKAPE pathogens clinical relevance of Pseudomonas aeruginosa strains |
| title | Pseudomonas aeruginosa reference strains PAO1 and PA14: A genomic, phenotypic, and therapeutic review |
| title_full | Pseudomonas aeruginosa reference strains PAO1 and PA14: A genomic, phenotypic, and therapeutic review |
| title_fullStr | Pseudomonas aeruginosa reference strains PAO1 and PA14: A genomic, phenotypic, and therapeutic review |
| title_full_unstemmed | Pseudomonas aeruginosa reference strains PAO1 and PA14: A genomic, phenotypic, and therapeutic review |
| title_short | Pseudomonas aeruginosa reference strains PAO1 and PA14: A genomic, phenotypic, and therapeutic review |
| title_sort | pseudomonas aeruginosa reference strains pao1 and pa14 a genomic phenotypic and therapeutic review |
| topic | Pseudomonas aeruginosa PAO1 PA14 Pseudomonas genome ESKAPE pathogens clinical relevance of Pseudomonas aeruginosa strains |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1023523/full |
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