Temperature depending bioelectrocatalysis current of multicopper oxidase from a hyperthermophilic archaeon Pyrobaculum aerophilum
The thermostable blue multicopper oxidase is stable at high temperature, however, because of this structural stability this enzyme has low activity. In this study, high electrochemical catalytic activity was achieved by immobilizing a thermostable blue multicopper oxidase from hyperthermophilic arch...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-04-01
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| Series: | Electrochemistry Communications |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1388248121000667 |
| _version_ | 1818447175320862720 |
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| author | Masato Tominaga Shino Nakao Makoto Takafuji Eiichiro Takamura Shin-ichiro Suye Takenori Satomura |
| author_facet | Masato Tominaga Shino Nakao Makoto Takafuji Eiichiro Takamura Shin-ichiro Suye Takenori Satomura |
| author_sort | Masato Tominaga |
| collection | DOAJ |
| description | The thermostable blue multicopper oxidase is stable at high temperature, however, because of this structural stability this enzyme has low activity. In this study, high electrochemical catalytic activity was achieved by immobilizing a thermostable blue multicopper oxidase from hyperthermophilic archaeon Pyrobaculum aerophilum (McoP) onto a single-walled carbon nanotube (SWCNT) electrode. The both results of electrochemical investigation for the McoP immobilized the SWCNT and spectroscopic measurements for the McoP solution suggested that a structural transition occured around 40 °C. |
| first_indexed | 2024-12-14T19:59:26Z |
| format | Article |
| id | doaj.art-f74c85d2dbd94f11a5b3c1bde4e7b9ca |
| institution | Directory Open Access Journal |
| issn | 1388-2481 |
| language | English |
| last_indexed | 2024-12-14T19:59:26Z |
| publishDate | 2021-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electrochemistry Communications |
| spelling | doaj.art-f74c85d2dbd94f11a5b3c1bde4e7b9ca2022-12-21T22:49:12ZengElsevierElectrochemistry Communications1388-24812021-04-01125106982Temperature depending bioelectrocatalysis current of multicopper oxidase from a hyperthermophilic archaeon Pyrobaculum aerophilumMasato Tominaga0Shino Nakao1Makoto Takafuji2Eiichiro Takamura3Shin-ichiro Suye4Takenori Satomura5Department of Chemistry and Applied Chemistry, Saga University, Saga 840-8502, Japan; Corresponding author.Department of Chemistry and Applied Chemistry, Saga University, Saga 840-8502, JapanFaculty of Advanced Science and Technology, Kumamoto University, Kumamoto 860-8555, JapanFaculty of Engineering, University of Fukui, Fukui 910-8507, JapanFaculty of Engineering, University of Fukui, Fukui 910-8507, JapanFaculty of Engineering, University of Fukui, Fukui 910-8507, JapanThe thermostable blue multicopper oxidase is stable at high temperature, however, because of this structural stability this enzyme has low activity. In this study, high electrochemical catalytic activity was achieved by immobilizing a thermostable blue multicopper oxidase from hyperthermophilic archaeon Pyrobaculum aerophilum (McoP) onto a single-walled carbon nanotube (SWCNT) electrode. The both results of electrochemical investigation for the McoP immobilized the SWCNT and spectroscopic measurements for the McoP solution suggested that a structural transition occured around 40 °C.http://www.sciencedirect.com/science/article/pii/S1388248121000667Blue multicopper oxidaseThermostableOxygen reductionSingle-walled carbon nanotube (SWCNT)Hyperthermophilic archaeonStructural transition |
| spellingShingle | Masato Tominaga Shino Nakao Makoto Takafuji Eiichiro Takamura Shin-ichiro Suye Takenori Satomura Temperature depending bioelectrocatalysis current of multicopper oxidase from a hyperthermophilic archaeon Pyrobaculum aerophilum Electrochemistry Communications Blue multicopper oxidase Thermostable Oxygen reduction Single-walled carbon nanotube (SWCNT) Hyperthermophilic archaeon Structural transition |
| title | Temperature depending bioelectrocatalysis current of multicopper oxidase from a hyperthermophilic archaeon Pyrobaculum aerophilum |
| title_full | Temperature depending bioelectrocatalysis current of multicopper oxidase from a hyperthermophilic archaeon Pyrobaculum aerophilum |
| title_fullStr | Temperature depending bioelectrocatalysis current of multicopper oxidase from a hyperthermophilic archaeon Pyrobaculum aerophilum |
| title_full_unstemmed | Temperature depending bioelectrocatalysis current of multicopper oxidase from a hyperthermophilic archaeon Pyrobaculum aerophilum |
| title_short | Temperature depending bioelectrocatalysis current of multicopper oxidase from a hyperthermophilic archaeon Pyrobaculum aerophilum |
| title_sort | temperature depending bioelectrocatalysis current of multicopper oxidase from a hyperthermophilic archaeon pyrobaculum aerophilum |
| topic | Blue multicopper oxidase Thermostable Oxygen reduction Single-walled carbon nanotube (SWCNT) Hyperthermophilic archaeon Structural transition |
| url | http://www.sciencedirect.com/science/article/pii/S1388248121000667 |
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