Gut microbiota shifts favorably with delivery of handwashing with soap and water treatment intervention in a prospective cohort (CHoBI7 trial)
Abstract Background Cholera can result in the expulsion of important microbiota from the gut and result in death if left untreated. The disease transmits mainly via drinking water carrying Vibrio cholerae; and household contacts (HHC) of cholera patients are at elevated risk during the first week of...
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| Format: | Article |
| Language: | English |
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BMC
2023-12-01
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| Series: | Journal of Health, Population and Nutrition |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s41043-023-00477-0 |
| _version_ | 1797376948399243264 |
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| author | Shirajum Monira Indrajeet Barman Fatema Tuz Jubyda Sk. Imran Ali Aminul Islam Kazi Mohammad Zillur Rahman Mahamud-ur Rashid Fatema-Tuz Johura Marzia Sultana Fatema Zohura Sazzadul Islam Bhuyian Tahmina Parvin David Sack Tahmeed Ahmed K M Saif-Ur-Rahman Maqsud Hossain Haruo Watanabe Christine Marie George Munirul Alam |
| author_facet | Shirajum Monira Indrajeet Barman Fatema Tuz Jubyda Sk. Imran Ali Aminul Islam Kazi Mohammad Zillur Rahman Mahamud-ur Rashid Fatema-Tuz Johura Marzia Sultana Fatema Zohura Sazzadul Islam Bhuyian Tahmina Parvin David Sack Tahmeed Ahmed K M Saif-Ur-Rahman Maqsud Hossain Haruo Watanabe Christine Marie George Munirul Alam |
| author_sort | Shirajum Monira |
| collection | DOAJ |
| description | Abstract Background Cholera can result in the expulsion of important microbiota from the gut and result in death if left untreated. The disease transmits mainly via drinking water carrying Vibrio cholerae; and household contacts (HHC) of cholera patients are at elevated risk during the first week of infection. The gut microbiota profiles of HHC-children of cholera patients at Dhaka city slums were investigated before (day 0) and after (day 8) delivery of chlorinated water as part of the major study ‘CHoBI7 trial (cholera-hospital-based intervention for 7 days)’. Result Results of sequencing and analysis of bacterial community DNA revealed the predominance of two bacterial phyla: Bacteroidetes and Firmicutes at day 0 with a relative abundance of 62 ± 6 (mean ± SEM%) and 32 ± 7, respectively. The pattern reversed at day 8 with a decreased relative abundance of Bacteroidetes (39 ± 12; p = 0.034) and an increased abundance of Firmicutes (49 ± 12; p = 0.057). Of 65 bacterial families confirmed at day 0, six belonging to Proteobacteria including Vibrionaceae disappeared at day 8. Interestingly, the relative abundance of four Firmicutes families—Lachnospiraceae, Bifidobacteriaceae, Clostridiaceae, and Ruminococcaceae was increased in all five study children at day 8. Conclusion The observed exclusion of pathogenic Proteobacteria and enhancement of beneficial Firmicutes in the gut of children delivered with chlorinated water as part of WASH intervention reflect a great promise of the CHoBI7 program in preventing cholera and improving child health. |
| first_indexed | 2024-03-08T19:45:57Z |
| format | Article |
| id | doaj.art-f74f39c98a144c0fa01d6dd354dc06af |
| institution | Directory Open Access Journal |
| issn | 2072-1315 |
| language | English |
| last_indexed | 2024-03-08T19:45:57Z |
| publishDate | 2023-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Health, Population and Nutrition |
| spelling | doaj.art-f74f39c98a144c0fa01d6dd354dc06af2023-12-24T12:22:14ZengBMCJournal of Health, Population and Nutrition2072-13152023-12-0142111110.1186/s41043-023-00477-0Gut microbiota shifts favorably with delivery of handwashing with soap and water treatment intervention in a prospective cohort (CHoBI7 trial)Shirajum Monira0Indrajeet Barman1Fatema Tuz Jubyda2Sk. Imran Ali3Aminul Islam4Kazi Mohammad Zillur Rahman5Mahamud-ur Rashid6Fatema-Tuz Johura7Marzia Sultana8Fatema Zohura9Sazzadul Islam Bhuyian10Tahmina Parvin11David Sack12Tahmeed Ahmed13K M Saif-Ur-Rahman14Maqsud Hossain15Haruo Watanabe16Christine Marie George17Munirul Alam18Molecular Ecology and Metagenomics Laboratory, Infectious Diseases Division, icddr,b, (International Centre for Diarrhoeal Diseases Research Bangladesh)Molecular Ecology and Metagenomics Laboratory, Infectious Diseases Division, icddr,b, (International Centre for Diarrhoeal Diseases Research Bangladesh)Molecular Ecology and Metagenomics Laboratory, Infectious Diseases Division, icddr,b, (International Centre for Diarrhoeal Diseases Research Bangladesh)Molecular Ecology and Metagenomics Laboratory, Infectious Diseases Division, icddr,b, (International Centre for Diarrhoeal Diseases Research Bangladesh)Molecular Ecology and Metagenomics Laboratory, Infectious Diseases Division, icddr,b, (International Centre for Diarrhoeal Diseases Research Bangladesh)Molecular Ecology and Metagenomics Laboratory, Infectious Diseases Division, icddr,b, (International Centre for Diarrhoeal Diseases Research Bangladesh)Molecular Ecology and Metagenomics Laboratory, Infectious Diseases Division, icddr,b, (International Centre for Diarrhoeal Diseases Research Bangladesh)Molecular Ecology and Metagenomics Laboratory, Infectious Diseases Division, icddr,b, (International Centre for Diarrhoeal Diseases Research Bangladesh)Molecular Ecology and Metagenomics Laboratory, Infectious Diseases Division, icddr,b, (International Centre for Diarrhoeal Diseases Research Bangladesh)Molecular Ecology and Metagenomics Laboratory, Infectious Diseases Division, icddr,b, (International Centre for Diarrhoeal Diseases Research Bangladesh)Molecular Ecology and Metagenomics Laboratory, Infectious Diseases Division, icddr,b, (International Centre for Diarrhoeal Diseases Research Bangladesh)Molecular Ecology and Metagenomics Laboratory, Infectious Diseases Division, icddr,b, (International Centre for Diarrhoeal Diseases Research Bangladesh)Department of International Health, Johns Hopkins Bloomberg School of Public HealthMolecular Ecology and Metagenomics Laboratory, Infectious Diseases Division, icddr,b, (International Centre for Diarrhoeal Diseases Research Bangladesh)Molecular Ecology and Metagenomics Laboratory, Infectious Diseases Division, icddr,b, (International Centre for Diarrhoeal Diseases Research Bangladesh)NSU Genome Research Institute, North South UniversityNational Institutes of Infectious Diseases (NIID)Department of International Health, Johns Hopkins Bloomberg School of Public HealthMolecular Ecology and Metagenomics Laboratory, Infectious Diseases Division, icddr,b, (International Centre for Diarrhoeal Diseases Research Bangladesh)Abstract Background Cholera can result in the expulsion of important microbiota from the gut and result in death if left untreated. The disease transmits mainly via drinking water carrying Vibrio cholerae; and household contacts (HHC) of cholera patients are at elevated risk during the first week of infection. The gut microbiota profiles of HHC-children of cholera patients at Dhaka city slums were investigated before (day 0) and after (day 8) delivery of chlorinated water as part of the major study ‘CHoBI7 trial (cholera-hospital-based intervention for 7 days)’. Result Results of sequencing and analysis of bacterial community DNA revealed the predominance of two bacterial phyla: Bacteroidetes and Firmicutes at day 0 with a relative abundance of 62 ± 6 (mean ± SEM%) and 32 ± 7, respectively. The pattern reversed at day 8 with a decreased relative abundance of Bacteroidetes (39 ± 12; p = 0.034) and an increased abundance of Firmicutes (49 ± 12; p = 0.057). Of 65 bacterial families confirmed at day 0, six belonging to Proteobacteria including Vibrionaceae disappeared at day 8. Interestingly, the relative abundance of four Firmicutes families—Lachnospiraceae, Bifidobacteriaceae, Clostridiaceae, and Ruminococcaceae was increased in all five study children at day 8. Conclusion The observed exclusion of pathogenic Proteobacteria and enhancement of beneficial Firmicutes in the gut of children delivered with chlorinated water as part of WASH intervention reflect a great promise of the CHoBI7 program in preventing cholera and improving child health.https://doi.org/10.1186/s41043-023-00477-0CholeraHandwashing with soapCHoBI7 WASH interventionChlorine-treated drinking waterHousehold-contact childrenGut microbiota |
| spellingShingle | Shirajum Monira Indrajeet Barman Fatema Tuz Jubyda Sk. Imran Ali Aminul Islam Kazi Mohammad Zillur Rahman Mahamud-ur Rashid Fatema-Tuz Johura Marzia Sultana Fatema Zohura Sazzadul Islam Bhuyian Tahmina Parvin David Sack Tahmeed Ahmed K M Saif-Ur-Rahman Maqsud Hossain Haruo Watanabe Christine Marie George Munirul Alam Gut microbiota shifts favorably with delivery of handwashing with soap and water treatment intervention in a prospective cohort (CHoBI7 trial) Journal of Health, Population and Nutrition Cholera Handwashing with soap CHoBI7 WASH intervention Chlorine-treated drinking water Household-contact children Gut microbiota |
| title | Gut microbiota shifts favorably with delivery of handwashing with soap and water treatment intervention in a prospective cohort (CHoBI7 trial) |
| title_full | Gut microbiota shifts favorably with delivery of handwashing with soap and water treatment intervention in a prospective cohort (CHoBI7 trial) |
| title_fullStr | Gut microbiota shifts favorably with delivery of handwashing with soap and water treatment intervention in a prospective cohort (CHoBI7 trial) |
| title_full_unstemmed | Gut microbiota shifts favorably with delivery of handwashing with soap and water treatment intervention in a prospective cohort (CHoBI7 trial) |
| title_short | Gut microbiota shifts favorably with delivery of handwashing with soap and water treatment intervention in a prospective cohort (CHoBI7 trial) |
| title_sort | gut microbiota shifts favorably with delivery of handwashing with soap and water treatment intervention in a prospective cohort chobi7 trial |
| topic | Cholera Handwashing with soap CHoBI7 WASH intervention Chlorine-treated drinking water Household-contact children Gut microbiota |
| url | https://doi.org/10.1186/s41043-023-00477-0 |
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