Acibenzolar-S-Methyl Activates Stomatal-Based Defense Systemically in Japanese Radish
Acibenzolar-S-methyl (ASM) is a well-known plant activator, which is a synthetic analog of salicylic acid (SA). Recently, copper fungicides and antibiotics are major strategies for controlling bacterial diseases. However, resistant strains have already been found. Therefore, there is an increasing d...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-10-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2020.565745/full |
| _version_ | 1818969767496646656 |
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| author | Nanami Sakata Takako Ishiga Shizuku Taniguchi Yasuhiro Ishiga |
| author_facet | Nanami Sakata Takako Ishiga Shizuku Taniguchi Yasuhiro Ishiga |
| author_sort | Nanami Sakata |
| collection | DOAJ |
| description | Acibenzolar-S-methyl (ASM) is a well-known plant activator, which is a synthetic analog of salicylic acid (SA). Recently, copper fungicides and antibiotics are major strategies for controlling bacterial diseases. However, resistant strains have already been found. Therefore, there is an increasing demand for sustainable new disease control strategies. We investigated the ASM disease control effect against Pseudomonas cannabina pv. alisalensis (Pcal), which causes bacterial blight on Japanese radish. In this study, we demonstrated that ASM effectively suppressed Pcal disease symptom development associated with reduced bacterial populations on Japanese radish leaves. Interestingly, we also demonstrated that ASM activated systemic acquired resistance (SAR), including stomatal-based defense on ASM-untreated upper and lower leaves. Reactive oxygen species (ROS) are essential second messengers in stomatal-based defense. We found that ASM induced stomatal closure by inducing ROS production through peroxidase. These results indicate that stomatal closure induced by ASM treatment is effective for preventing Pcal pathogen invasion into plants, and in turn reduction of disease development. |
| first_indexed | 2024-12-20T14:25:49Z |
| format | Article |
| id | doaj.art-f752e44b1f4844c5b1c74a2b7171f036 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-20T14:25:49Z |
| publishDate | 2020-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-f752e44b1f4844c5b1c74a2b7171f0362022-12-21T19:37:48ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-10-011110.3389/fpls.2020.565745565745Acibenzolar-S-Methyl Activates Stomatal-Based Defense Systemically in Japanese RadishNanami Sakata0Takako Ishiga1Shizuku Taniguchi2Yasuhiro Ishiga3Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, JapanFaculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, JapanSyngenta Japan, Ushiku, JapanFaculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, JapanAcibenzolar-S-methyl (ASM) is a well-known plant activator, which is a synthetic analog of salicylic acid (SA). Recently, copper fungicides and antibiotics are major strategies for controlling bacterial diseases. However, resistant strains have already been found. Therefore, there is an increasing demand for sustainable new disease control strategies. We investigated the ASM disease control effect against Pseudomonas cannabina pv. alisalensis (Pcal), which causes bacterial blight on Japanese radish. In this study, we demonstrated that ASM effectively suppressed Pcal disease symptom development associated with reduced bacterial populations on Japanese radish leaves. Interestingly, we also demonstrated that ASM activated systemic acquired resistance (SAR), including stomatal-based defense on ASM-untreated upper and lower leaves. Reactive oxygen species (ROS) are essential second messengers in stomatal-based defense. We found that ASM induced stomatal closure by inducing ROS production through peroxidase. These results indicate that stomatal closure induced by ASM treatment is effective for preventing Pcal pathogen invasion into plants, and in turn reduction of disease development.https://www.frontiersin.org/articles/10.3389/fpls.2020.565745/fullAcibenzolar-S-methylPseudomonas cannabina pv. alisalensisJapanese radishstomatal-based defensesystemic acquired resistanceplant defense activator |
| spellingShingle | Nanami Sakata Takako Ishiga Shizuku Taniguchi Yasuhiro Ishiga Acibenzolar-S-Methyl Activates Stomatal-Based Defense Systemically in Japanese Radish Frontiers in Plant Science Acibenzolar-S-methyl Pseudomonas cannabina pv. alisalensis Japanese radish stomatal-based defense systemic acquired resistance plant defense activator |
| title | Acibenzolar-S-Methyl Activates Stomatal-Based Defense Systemically in Japanese Radish |
| title_full | Acibenzolar-S-Methyl Activates Stomatal-Based Defense Systemically in Japanese Radish |
| title_fullStr | Acibenzolar-S-Methyl Activates Stomatal-Based Defense Systemically in Japanese Radish |
| title_full_unstemmed | Acibenzolar-S-Methyl Activates Stomatal-Based Defense Systemically in Japanese Radish |
| title_short | Acibenzolar-S-Methyl Activates Stomatal-Based Defense Systemically in Japanese Radish |
| title_sort | acibenzolar s methyl activates stomatal based defense systemically in japanese radish |
| topic | Acibenzolar-S-methyl Pseudomonas cannabina pv. alisalensis Japanese radish stomatal-based defense systemic acquired resistance plant defense activator |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2020.565745/full |
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