Catalysis of proline isomerization and molecular chaperone activity in a tug-of-war
Cyclophilin A (CypA) is a peptidylprolyl isomerase that also has chaperone activity and interacts with the intrinsically disordered protein α-Synuclein (aSyn). Here, the authors combine NMR measurements and biochemical experiments to characterise the interplay between the catalysis of proline isomer...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2020-11-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-19844-0 |
| _version_ | 1818991926109536256 |
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| author | Filippo Favretto David Flores Jeremy D. Baker Timo Strohäker Loren B. Andreas Laura J. Blair Stefan Becker Markus Zweckstetter |
| author_facet | Filippo Favretto David Flores Jeremy D. Baker Timo Strohäker Loren B. Andreas Laura J. Blair Stefan Becker Markus Zweckstetter |
| author_sort | Filippo Favretto |
| collection | DOAJ |
| description | Cyclophilin A (CypA) is a peptidylprolyl isomerase that also has chaperone activity and interacts with the intrinsically disordered protein α-Synuclein (aSyn). Here, the authors combine NMR measurements and biochemical experiments to characterise the interplay between the catalysis of proline isomerization and molecular chaperone activity of CypA and find that both activities have opposing effects on aSyn and further show that the that cis/trans isomerization outpowers the holding activity of CypA. |
| first_indexed | 2024-12-20T20:18:01Z |
| format | Article |
| id | doaj.art-f754601d5f914b7aaa2c4a5fef0fc9ba |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-20T20:18:01Z |
| publishDate | 2020-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-f754601d5f914b7aaa2c4a5fef0fc9ba2022-12-21T19:27:39ZengNature PortfolioNature Communications2041-17232020-11-0111111210.1038/s41467-020-19844-0Catalysis of proline isomerization and molecular chaperone activity in a tug-of-warFilippo Favretto0David Flores1Jeremy D. Baker2Timo Strohäker3Loren B. Andreas4Laura J. Blair5Stefan Becker6Markus Zweckstetter7German Center for Neurodegenerative Diseases (DZNE)German Center for Neurodegenerative Diseases (DZNE)Department of Molecular Medicine, Morsani College of Medicine, USF Health Byrd Alzheimer’s Institute, University of South FloridaGerman Center for Neurodegenerative Diseases (DZNE)Department for NMR-based Structural Biology, Max Planck Institute for Biophysical ChemistryDepartment of Molecular Medicine, Morsani College of Medicine, USF Health Byrd Alzheimer’s Institute, University of South FloridaDepartment for NMR-based Structural Biology, Max Planck Institute for Biophysical ChemistryGerman Center for Neurodegenerative Diseases (DZNE)Cyclophilin A (CypA) is a peptidylprolyl isomerase that also has chaperone activity and interacts with the intrinsically disordered protein α-Synuclein (aSyn). Here, the authors combine NMR measurements and biochemical experiments to characterise the interplay between the catalysis of proline isomerization and molecular chaperone activity of CypA and find that both activities have opposing effects on aSyn and further show that the that cis/trans isomerization outpowers the holding activity of CypA.https://doi.org/10.1038/s41467-020-19844-0 |
| spellingShingle | Filippo Favretto David Flores Jeremy D. Baker Timo Strohäker Loren B. Andreas Laura J. Blair Stefan Becker Markus Zweckstetter Catalysis of proline isomerization and molecular chaperone activity in a tug-of-war Nature Communications |
| title | Catalysis of proline isomerization and molecular chaperone activity in a tug-of-war |
| title_full | Catalysis of proline isomerization and molecular chaperone activity in a tug-of-war |
| title_fullStr | Catalysis of proline isomerization and molecular chaperone activity in a tug-of-war |
| title_full_unstemmed | Catalysis of proline isomerization and molecular chaperone activity in a tug-of-war |
| title_short | Catalysis of proline isomerization and molecular chaperone activity in a tug-of-war |
| title_sort | catalysis of proline isomerization and molecular chaperone activity in a tug of war |
| url | https://doi.org/10.1038/s41467-020-19844-0 |
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