Leaf antioxidant machinery stimulation by Meloidogyne javanica infestation: A case study on Cucumis melo seedlings
Following nematode infestation, leaf-localized responses are vital not only as early infection signs but also as an indication of actions required to preserve crop productivity. In this context, the leaf-level physiological responses of cantaloupe seedlings were evaluated after 40 consecutive days o...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-01-01
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| Series: | Plant Stress |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667064X21000014 |
| _version_ | 1829478626830057472 |
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| author | Georgios Tsaniklidis Theocharis Chatzistathis Dimitrios Fanourakis Nikolaos Nikoloudakis Anastasios Kotsiras Costas Delis Emmanuel A. Tzortzakakis |
| author_facet | Georgios Tsaniklidis Theocharis Chatzistathis Dimitrios Fanourakis Nikolaos Nikoloudakis Anastasios Kotsiras Costas Delis Emmanuel A. Tzortzakakis |
| author_sort | Georgios Tsaniklidis |
| collection | DOAJ |
| description | Following nematode infestation, leaf-localized responses are vital not only as early infection signs but also as an indication of actions required to preserve crop productivity. In this context, the leaf-level physiological responses of cantaloupe seedlings were evaluated after 40 consecutive days of cultivation under different Meloidogyne javanica juveniles (J2s) inoculation regimes (0, 100, and 850 J2s plant−1). Leaf growth parameters and photosynthetic pigments (chlorophyll, carotenoids) content were not affected by J2s infestation, while foliar Potassium concentration significantly declined. The RuBIsCo activase gene expression was negatively associated with the J2s inoculation level. Total flavonoids, total phenolics, lipid peroxidation indexes, phenylalanine ammonia-lyase activity, as well as, ascorbate peroxidase gene transcription, were higher in the inoculated plants (regardless of the J2s inoculant level). Taken together, present data indicate that J2s infestation impacts primarily the Potassium levels in leaves. Additionally, a substantial stimulation of the plant antioxidant machinery (independent of the nematode infestation intensity) is triggered. Finally, it was established that the transcriptional regulation of the RuBIsCo activase 1 gene under biotic stress has the capacity to be employed as a potential stress indicator marker. |
| first_indexed | 2024-12-14T17:18:36Z |
| format | Article |
| id | doaj.art-f7559eb040af4c0e91a1ec15789b0fdc |
| institution | Directory Open Access Journal |
| issn | 2667-064X |
| language | English |
| last_indexed | 2024-12-14T17:18:36Z |
| publishDate | 2021-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Plant Stress |
| spelling | doaj.art-f7559eb040af4c0e91a1ec15789b0fdc2022-12-21T22:53:22ZengElsevierPlant Stress2667-064X2021-01-011100002Leaf antioxidant machinery stimulation by Meloidogyne javanica infestation: A case study on Cucumis melo seedlingsGeorgios Tsaniklidis0Theocharis Chatzistathis1Dimitrios Fanourakis2Nikolaos Nikoloudakis3Anastasios Kotsiras4Costas Delis5Emmanuel A. Tzortzakakis6Hellenic Agricultural Organization (H.A.O.)-“DIMITRA”, Institute of Olive Tree, Subtropical Crops and Viticulture, Department of Viticulture, Vegetable Crops, Floriculture and Plant Protection, 32A Kastorias street, 71307, Heraklion, Crete, Greece; Corresponding author.Hellenic Agricultural Organization (H.A.O.)-“DIMITRA”, Institute of Soil and Water Resources, 57001, Thessaloniki (Thermi), GreeceHellenic Mediterranean University, Department of Agriculture, Laboratory of Quality and Safety of Agricultural Products, Landscape and Environment, Specialization of Floriculture and Landscape Architecture, Estavromenos, Heraklion, Crete, 71004, GreeceCyprus University of Technology, Department of Agricultural Sciences, Biotechnology and Food Science, Limassol, CyprusDepartment of Agriculture, University of the Peloponnese, 24100 Antikalamos, Kalamata, GreeceDepartment of Agriculture, University of the Peloponnese, 24100 Antikalamos, Kalamata, GreeceHellenic Agricultural Organization (H.A.O.)-“DIMITRA”, Institute of Olive Tree, Subtropical Crops and Viticulture, Department of Viticulture, Vegetable Crops, Floriculture and Plant Protection, 32A Kastorias street, 71307, Heraklion, Crete, GreeceFollowing nematode infestation, leaf-localized responses are vital not only as early infection signs but also as an indication of actions required to preserve crop productivity. In this context, the leaf-level physiological responses of cantaloupe seedlings were evaluated after 40 consecutive days of cultivation under different Meloidogyne javanica juveniles (J2s) inoculation regimes (0, 100, and 850 J2s plant−1). Leaf growth parameters and photosynthetic pigments (chlorophyll, carotenoids) content were not affected by J2s infestation, while foliar Potassium concentration significantly declined. The RuBIsCo activase gene expression was negatively associated with the J2s inoculation level. Total flavonoids, total phenolics, lipid peroxidation indexes, phenylalanine ammonia-lyase activity, as well as, ascorbate peroxidase gene transcription, were higher in the inoculated plants (regardless of the J2s inoculant level). Taken together, present data indicate that J2s infestation impacts primarily the Potassium levels in leaves. Additionally, a substantial stimulation of the plant antioxidant machinery (independent of the nematode infestation intensity) is triggered. Finally, it was established that the transcriptional regulation of the RuBIsCo activase 1 gene under biotic stress has the capacity to be employed as a potential stress indicator marker.http://www.sciencedirect.com/science/article/pii/S2667064X21000014Antioxidant defenseBiotic stressMelonMeloidogyne javanicaPhotosynthesisRoot-knot nematode |
| spellingShingle | Georgios Tsaniklidis Theocharis Chatzistathis Dimitrios Fanourakis Nikolaos Nikoloudakis Anastasios Kotsiras Costas Delis Emmanuel A. Tzortzakakis Leaf antioxidant machinery stimulation by Meloidogyne javanica infestation: A case study on Cucumis melo seedlings Plant Stress Antioxidant defense Biotic stress Melon Meloidogyne javanica Photosynthesis Root-knot nematode |
| title | Leaf antioxidant machinery stimulation by Meloidogyne javanica infestation: A case study on Cucumis melo seedlings |
| title_full | Leaf antioxidant machinery stimulation by Meloidogyne javanica infestation: A case study on Cucumis melo seedlings |
| title_fullStr | Leaf antioxidant machinery stimulation by Meloidogyne javanica infestation: A case study on Cucumis melo seedlings |
| title_full_unstemmed | Leaf antioxidant machinery stimulation by Meloidogyne javanica infestation: A case study on Cucumis melo seedlings |
| title_short | Leaf antioxidant machinery stimulation by Meloidogyne javanica infestation: A case study on Cucumis melo seedlings |
| title_sort | leaf antioxidant machinery stimulation by meloidogyne javanica infestation a case study on cucumis melo seedlings |
| topic | Antioxidant defense Biotic stress Melon Meloidogyne javanica Photosynthesis Root-knot nematode |
| url | http://www.sciencedirect.com/science/article/pii/S2667064X21000014 |
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