Genomic Analysis of <i>Aeromonas veronii</i> C198, a Novel <i>Mcr-3.41</i>-Harboring Isolate from a Patient with Septicemia in Thailand
The resistance of Gram-negative bacteria to colistin, mediated by plasmid-borne <i>mcr</i> genes, is an emerging public health concern. The complete genome sequence (4.55 Mb) of a clinical isolate of <i>Aeromonas veronii</i> biovar <i>veronii</i> obtained from a p...
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MDPI AG
2020-12-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/9/12/1031 |
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| author | Rujirat Hatrongjit Anusak Kerdsin Dan Takeuchi Thidathip Wongsurawat Piroon Jenjaroenpun Peechanika Chopjitt Parichart Boueroy Yukihiro Akeda Shigeyuki Hamada |
| author_facet | Rujirat Hatrongjit Anusak Kerdsin Dan Takeuchi Thidathip Wongsurawat Piroon Jenjaroenpun Peechanika Chopjitt Parichart Boueroy Yukihiro Akeda Shigeyuki Hamada |
| author_sort | Rujirat Hatrongjit |
| collection | DOAJ |
| description | The resistance of Gram-negative bacteria to colistin, mediated by plasmid-borne <i>mcr</i> genes, is an emerging public health concern. The complete genome sequence (4.55 Mb) of a clinical isolate of <i>Aeromonas veronii</i> biovar <i>veronii</i> obtained from a patient with septicemia was determined using short-read and long-read platforms. This isolate (C198) was found to harbor a novel <i>mcr-3</i> gene, designated <i>mcr-3.41</i>. Isolate C198 revealed adjacent <i>mcr-3.41</i> and <i>mcr-3</i>-like genes. It contained one chromosome and two plasmids, both of which encoded a RepB replication protein. Other antimicrobial resistance genes, including <i>bla<sub>cphA3</sub>, bla<sub>OXA-12</sub>, tetA, rsmA,</i> and adeF, were also present. Isolate C198 was resistant to amoxicillin–clavulanate, ampicillin–sulbactam and tetracycline, and showed intermediate resistance to trimethoprim–sulfamethoxazole. The isolate was susceptible to piperacillin–tazobactam, carbapenem, third-generation cephalosporins, fluoroquinolones, chloramphenicol, and aminoglycosides. Putative virulence genes in the C198 genome encoded type II, III, and VI secretion systems; type IV <i>Aeromonas</i> pili; and type I fimbria, flagella, hemagglutinin, aerolysin, and hemolysins. Multilocus sequence typing revealed a novel sequence type (ST), ST720 for C198. Phylogenetic analysis of the single nucleotide polymorphisms in C198 demonstrated that the strain was closely related to <i>A. veronii</i> 17ISAe. The present study provides insights into the genomic characteristics of human <i>A. veronii</i> isolates. |
| first_indexed | 2024-03-10T14:13:12Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T14:13:12Z |
| publishDate | 2020-12-01 |
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| spelling | doaj.art-f75b391b5d5149c9a4e1908dd00b62632023-11-21T00:02:10ZengMDPI AGPathogens2076-08172020-12-01912103110.3390/pathogens9121031Genomic Analysis of <i>Aeromonas veronii</i> C198, a Novel <i>Mcr-3.41</i>-Harboring Isolate from a Patient with Septicemia in ThailandRujirat Hatrongjit0Anusak Kerdsin1Dan Takeuchi2Thidathip Wongsurawat3Piroon Jenjaroenpun4Peechanika Chopjitt5Parichart Boueroy6Yukihiro Akeda7Shigeyuki Hamada8Department of General Sciences, Faculty of Science and Engineering, Chalermphrakiat Sakon Nakhon province campus, Kasetsart University, Sakon Nakhon 47000, ThailandFaculty of Public Health, Chalermphrakiat Sakon Nakhon province campus, Kasetsart University, Sakon Nakhon 47000, ThailandResearch Institute for Microbial Diseases, Osaka University, Osaka 565-0871, JapanDepartment of Biomedical Informatics, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USADepartment of Biomedical Informatics, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USAFaculty of Public Health, Chalermphrakiat Sakon Nakhon province campus, Kasetsart University, Sakon Nakhon 47000, ThailandFaculty of Public Health, Chalermphrakiat Sakon Nakhon province campus, Kasetsart University, Sakon Nakhon 47000, ThailandResearch Institute for Microbial Diseases, Osaka University, Osaka 565-0871, JapanResearch Institute for Microbial Diseases, Osaka University, Osaka 565-0871, JapanThe resistance of Gram-negative bacteria to colistin, mediated by plasmid-borne <i>mcr</i> genes, is an emerging public health concern. The complete genome sequence (4.55 Mb) of a clinical isolate of <i>Aeromonas veronii</i> biovar <i>veronii</i> obtained from a patient with septicemia was determined using short-read and long-read platforms. This isolate (C198) was found to harbor a novel <i>mcr-3</i> gene, designated <i>mcr-3.41</i>. Isolate C198 revealed adjacent <i>mcr-3.41</i> and <i>mcr-3</i>-like genes. It contained one chromosome and two plasmids, both of which encoded a RepB replication protein. Other antimicrobial resistance genes, including <i>bla<sub>cphA3</sub>, bla<sub>OXA-12</sub>, tetA, rsmA,</i> and adeF, were also present. Isolate C198 was resistant to amoxicillin–clavulanate, ampicillin–sulbactam and tetracycline, and showed intermediate resistance to trimethoprim–sulfamethoxazole. The isolate was susceptible to piperacillin–tazobactam, carbapenem, third-generation cephalosporins, fluoroquinolones, chloramphenicol, and aminoglycosides. Putative virulence genes in the C198 genome encoded type II, III, and VI secretion systems; type IV <i>Aeromonas</i> pili; and type I fimbria, flagella, hemagglutinin, aerolysin, and hemolysins. Multilocus sequence typing revealed a novel sequence type (ST), ST720 for C198. Phylogenetic analysis of the single nucleotide polymorphisms in C198 demonstrated that the strain was closely related to <i>A. veronii</i> 17ISAe. The present study provides insights into the genomic characteristics of human <i>A. veronii</i> isolates.https://www.mdpi.com/2076-0817/9/12/1031<i>Aeromonas veronii</i>colistin<i>mcr</i>genomeThailand |
| spellingShingle | Rujirat Hatrongjit Anusak Kerdsin Dan Takeuchi Thidathip Wongsurawat Piroon Jenjaroenpun Peechanika Chopjitt Parichart Boueroy Yukihiro Akeda Shigeyuki Hamada Genomic Analysis of <i>Aeromonas veronii</i> C198, a Novel <i>Mcr-3.41</i>-Harboring Isolate from a Patient with Septicemia in Thailand Pathogens <i>Aeromonas veronii</i> colistin <i>mcr</i> genome Thailand |
| title | Genomic Analysis of <i>Aeromonas veronii</i> C198, a Novel <i>Mcr-3.41</i>-Harboring Isolate from a Patient with Septicemia in Thailand |
| title_full | Genomic Analysis of <i>Aeromonas veronii</i> C198, a Novel <i>Mcr-3.41</i>-Harboring Isolate from a Patient with Septicemia in Thailand |
| title_fullStr | Genomic Analysis of <i>Aeromonas veronii</i> C198, a Novel <i>Mcr-3.41</i>-Harboring Isolate from a Patient with Septicemia in Thailand |
| title_full_unstemmed | Genomic Analysis of <i>Aeromonas veronii</i> C198, a Novel <i>Mcr-3.41</i>-Harboring Isolate from a Patient with Septicemia in Thailand |
| title_short | Genomic Analysis of <i>Aeromonas veronii</i> C198, a Novel <i>Mcr-3.41</i>-Harboring Isolate from a Patient with Septicemia in Thailand |
| title_sort | genomic analysis of i aeromonas veronii i c198 a novel i mcr 3 41 i harboring isolate from a patient with septicemia in thailand |
| topic | <i>Aeromonas veronii</i> colistin <i>mcr</i> genome Thailand |
| url | https://www.mdpi.com/2076-0817/9/12/1031 |
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