Immunoreactivities of AR, ERα, ERβ and aromatase in the nuptial pad of Chinese brown frog (<em>Rana dybowskii</em>) during pre-hibernation and the breeding period
There is a prominent local raised pad called nuptial pad on the forelimb of Chinese brown frog (Rana dybowskii), which is hypothetically concluded as an enhancement of the grip and a spreader of pheromone during the amplexus. In this study, we investigated the immunolocalization and protein expressi...
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| Format: | Article |
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PAGEPress Publications
2021-04-01
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| Serier: | European Journal of Histochemistry |
| Fag: | |
| Online adgang: | https://www.ejh.it/index.php/ejh/article/view/3206 |
| _version_ | 1830277635537633280 |
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| author | Zeqi Tang Yuan Chen Baolong Ren Xi Wang Haolin Zhang Yingying Han Zhengrong Yuan Qiang Weng |
| author_facet | Zeqi Tang Yuan Chen Baolong Ren Xi Wang Haolin Zhang Yingying Han Zhengrong Yuan Qiang Weng |
| author_sort | Zeqi Tang |
| collection | DOAJ |
| description | There is a prominent local raised pad called nuptial pad on the forelimb of Chinese brown frog (Rana dybowskii), which is hypothetically concluded as an enhancement of the grip and a spreader of pheromone during the amplexus. In this study, we investigated the immunolocalization and protein expression levels of AR, ERα, ERβ and aromatase in the nuptial pad of R. dybowskii during pre-hibernation and the breeding period. Histologically, the annual development of the nuptial pad in R. dybowskii is manifested as the larger area of specialized mucous gland and the longer length of papillary epidermal projection during the breeding period. AR, ERα, ERβ and aromatase are present in the stratum granulosum, stratum spinosum, stratum basale and the secretory portion of specialized mucous glands during both periods. Western blotting results confirmed that AR, ERα and ERβ protein levels are higher during pre-hibernation than those during the breeding season. These results suggest that nuptial pad is the direct target organ of androgen and estrogen. Androgen may participate in the regulation of annual development and glandular function of nuptial pad, and estrogen may play an endocrine, autocrine or paracrine role during pre-hibernation and the breeding period. |
| first_indexed | 2024-12-19T01:05:53Z |
| format | Article |
| id | doaj.art-f75e42656b4c4a9995bb935a3dacb51d |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-12-19T01:05:53Z |
| publishDate | 2021-04-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-f75e42656b4c4a9995bb935a3dacb51d2022-12-21T20:43:20ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062021-04-0165210.4081/ejh.2021.3206Immunoreactivities of AR, ERα, ERβ and aromatase in the nuptial pad of Chinese brown frog (<em>Rana dybowskii</em>) during pre-hibernation and the breeding periodZeqi Tang0Yuan Chen1Baolong Ren2Xi Wang3Haolin Zhang4Yingying Han5Zhengrong Yuan6Qiang Weng7Laboratory of Animal Physiology, College of Biological Sciences and Technology, Beijing Forestry University, BeijingLaboratory of Animal Physiology, College of Biological Sciences and Technology, Beijing Forestry University, BeijingLaboratory of Animal Physiology, College of Biological Sciences and Technology, Beijing Forestry University, BeijingLaboratory of Animal Physiology, College of Biological Sciences and Technology, Beijing Forestry University, BeijingLaboratory of Animal Physiology, College of Biological Sciences and Technology, Beijing Forestry University, BeijingLaboratory of Animal Physiology, College of Biological Sciences and Technology, Beijing Forestry University, BeijingLaboratory of Animal Physiology, College of Biological Sciences and Technology, Beijing Forestry University, BeijingLaboratory of Animal Physiology, College of Biological Sciences and Technology, Beijing Forestry University, BeijingThere is a prominent local raised pad called nuptial pad on the forelimb of Chinese brown frog (Rana dybowskii), which is hypothetically concluded as an enhancement of the grip and a spreader of pheromone during the amplexus. In this study, we investigated the immunolocalization and protein expression levels of AR, ERα, ERβ and aromatase in the nuptial pad of R. dybowskii during pre-hibernation and the breeding period. Histologically, the annual development of the nuptial pad in R. dybowskii is manifested as the larger area of specialized mucous gland and the longer length of papillary epidermal projection during the breeding period. AR, ERα, ERβ and aromatase are present in the stratum granulosum, stratum spinosum, stratum basale and the secretory portion of specialized mucous glands during both periods. Western blotting results confirmed that AR, ERα and ERβ protein levels are higher during pre-hibernation than those during the breeding season. These results suggest that nuptial pad is the direct target organ of androgen and estrogen. Androgen may participate in the regulation of annual development and glandular function of nuptial pad, and estrogen may play an endocrine, autocrine or paracrine role during pre-hibernation and the breeding period.https://www.ejh.it/index.php/ejh/article/view/3206Androgen receptoraromataseestrogen receptornuptial padRana dybowskii |
| spellingShingle | Zeqi Tang Yuan Chen Baolong Ren Xi Wang Haolin Zhang Yingying Han Zhengrong Yuan Qiang Weng Immunoreactivities of AR, ERα, ERβ and aromatase in the nuptial pad of Chinese brown frog (<em>Rana dybowskii</em>) during pre-hibernation and the breeding period European Journal of Histochemistry Androgen receptor aromatase estrogen receptor nuptial pad Rana dybowskii |
| title | Immunoreactivities of AR, ERα, ERβ and aromatase in the nuptial pad of Chinese brown frog (<em>Rana dybowskii</em>) during pre-hibernation and the breeding period |
| title_full | Immunoreactivities of AR, ERα, ERβ and aromatase in the nuptial pad of Chinese brown frog (<em>Rana dybowskii</em>) during pre-hibernation and the breeding period |
| title_fullStr | Immunoreactivities of AR, ERα, ERβ and aromatase in the nuptial pad of Chinese brown frog (<em>Rana dybowskii</em>) during pre-hibernation and the breeding period |
| title_full_unstemmed | Immunoreactivities of AR, ERα, ERβ and aromatase in the nuptial pad of Chinese brown frog (<em>Rana dybowskii</em>) during pre-hibernation and the breeding period |
| title_short | Immunoreactivities of AR, ERα, ERβ and aromatase in the nuptial pad of Chinese brown frog (<em>Rana dybowskii</em>) during pre-hibernation and the breeding period |
| title_sort | immunoreactivities of ar erα erβ and aromatase in the nuptial pad of chinese brown frog em rana dybowskii em during pre hibernation and the breeding period |
| topic | Androgen receptor aromatase estrogen receptor nuptial pad Rana dybowskii |
| url | https://www.ejh.it/index.php/ejh/article/view/3206 |
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