Constitutive Expression of OsGH3.1 Reduces Auxin Content and Enhances Defense Response and Resistance to a Fungal Pathogen in Rice
GH3 genes are main components of the hormonal mechanism regulating growth and development and, hence, are deeply involved in a broad range of physiological processes. They are implicated in hormonal homeostasis through the conjugation to amino acids of the free form of essential plant growth regulat...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2009-02-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-22-2-0201 |
| _version_ | 1811277721701974016 |
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| author | Concha Domingo Fernando Andrés Didier Tharreau Domingo J. Iglesias Manuel Talón |
| author_facet | Concha Domingo Fernando Andrés Didier Tharreau Domingo J. Iglesias Manuel Talón |
| author_sort | Concha Domingo |
| collection | DOAJ |
| description | GH3 genes are main components of the hormonal mechanism regulating growth and development and, hence, are deeply involved in a broad range of physiological processes. They are implicated in hormonal homeostasis through the conjugation to amino acids of the free form of essential plant growth regulators such as indoleacetic and jasmonic acids. In this work, we showed that OsGH3.1 overexpression in rice caused dwarfism and significantly reduced both free auxin content and cell elongation. Functional classification of the transcriptomic profiling revealed that most genes involved in auxin biosynthesis and auxin signaling inhibition were induced and repressed, respectively. Many genes related to cell organization and biogenesis were also significantly downregulated. The survey also showed that, although the response to abiotic stresses was not clearly stimulated, OsGH3.1 overexpression did activate a significant number of defense-related genes. In successive bioassays, it was demonstrated that the resistance of rice plants to pathogen infection, evaluated with two different Magnaporthe grisea strains, was higher in the transformants overexpressing OsGH3.1. Taken together, these results indicate that OsGH3.1 overexpression reduces auxin content, inhibits cell growth and cell wall loosening, and enhances resistance to a fungal pathogen. Our results provide evidence that auxin homeostasis can regulate the activation of the defense response in rice. |
| first_indexed | 2024-04-13T00:21:52Z |
| format | Article |
| id | doaj.art-f75ee02edae943ca88683430b4a87a24 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T00:21:52Z |
| publishDate | 2009-02-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-f75ee02edae943ca88683430b4a87a242022-12-22T03:10:44ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062009-02-0122220121010.1094/MPMI-22-2-0201Constitutive Expression of OsGH3.1 Reduces Auxin Content and Enhances Defense Response and Resistance to a Fungal Pathogen in RiceConcha DomingoFernando AndrésDidier TharreauDomingo J. IglesiasManuel TalónGH3 genes are main components of the hormonal mechanism regulating growth and development and, hence, are deeply involved in a broad range of physiological processes. They are implicated in hormonal homeostasis through the conjugation to amino acids of the free form of essential plant growth regulators such as indoleacetic and jasmonic acids. In this work, we showed that OsGH3.1 overexpression in rice caused dwarfism and significantly reduced both free auxin content and cell elongation. Functional classification of the transcriptomic profiling revealed that most genes involved in auxin biosynthesis and auxin signaling inhibition were induced and repressed, respectively. Many genes related to cell organization and biogenesis were also significantly downregulated. The survey also showed that, although the response to abiotic stresses was not clearly stimulated, OsGH3.1 overexpression did activate a significant number of defense-related genes. In successive bioassays, it was demonstrated that the resistance of rice plants to pathogen infection, evaluated with two different Magnaporthe grisea strains, was higher in the transformants overexpressing OsGH3.1. Taken together, these results indicate that OsGH3.1 overexpression reduces auxin content, inhibits cell growth and cell wall loosening, and enhances resistance to a fungal pathogen. Our results provide evidence that auxin homeostasis can regulate the activation of the defense response in rice.https://apsjournals.apsnet.org/doi/10.1094/MPMI-22-2-0201fungal resistance |
| spellingShingle | Concha Domingo Fernando Andrés Didier Tharreau Domingo J. Iglesias Manuel Talón Constitutive Expression of OsGH3.1 Reduces Auxin Content and Enhances Defense Response and Resistance to a Fungal Pathogen in Rice Molecular Plant-Microbe Interactions fungal resistance |
| title | Constitutive Expression of OsGH3.1 Reduces Auxin Content and Enhances Defense Response and Resistance to a Fungal Pathogen in Rice |
| title_full | Constitutive Expression of OsGH3.1 Reduces Auxin Content and Enhances Defense Response and Resistance to a Fungal Pathogen in Rice |
| title_fullStr | Constitutive Expression of OsGH3.1 Reduces Auxin Content and Enhances Defense Response and Resistance to a Fungal Pathogen in Rice |
| title_full_unstemmed | Constitutive Expression of OsGH3.1 Reduces Auxin Content and Enhances Defense Response and Resistance to a Fungal Pathogen in Rice |
| title_short | Constitutive Expression of OsGH3.1 Reduces Auxin Content and Enhances Defense Response and Resistance to a Fungal Pathogen in Rice |
| title_sort | constitutive expression of osgh3 1 reduces auxin content and enhances defense response and resistance to a fungal pathogen in rice |
| topic | fungal resistance |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-22-2-0201 |
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