Surface-associated lipid droplets: an intermediate site for lipid transport in human adipocytes?
Adipose tissue plays a major role in regulating whole-body energy metabolism. While the biochemical processes regulating storage and release of excess energy are well known, the temporal organization of these events is much less defined. In this study, we have characterized the presence of small sur...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2020-01-01
|
| Series: | Adipocyte |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/21623945.2020.1838684 |
| _version_ | 1818654997419655168 |
|---|---|
| author | Björn Morén Claes Fryklund Karin Stenkula |
| author_facet | Björn Morén Claes Fryklund Karin Stenkula |
| author_sort | Björn Morén |
| collection | DOAJ |
| description | Adipose tissue plays a major role in regulating whole-body energy metabolism. While the biochemical processes regulating storage and release of excess energy are well known, the temporal organization of these events is much less defined. In this study, we have characterized the presence of small surface-associated lipid droplets, distinct from the central droplet, in primary human adipocytes. Based on microscopy analyses, we illustrate the distribution of mitochondria, endoplasmic reticulum and lysosomes in the vicinity of these specialized lipid droplets. Ultrastructure analysis confirmed the presence of small droplets in intact adipose tissue. Further, CIDEC, known to bind and regulate lipid droplet expansion, clearly localized at these lipid droplets. Neither acute or prolonged stimulation with insulin or isoprenaline, or pharmacologic intervention to suppress lipid flux, affected the presence of these lipid droplets. Still, phosphorylated perilipin and hormone-sensitive lipase accumulated at these droplets following adrenergic stimuli, which supports metabolic activity at these locations. Altogether, we propose these lipid droplet clusters represent an intermediate site involved in lipid transport in primary adipocytes. |
| first_indexed | 2024-12-17T03:02:41Z |
| format | Article |
| id | doaj.art-f7604469915947ee89d7c648cfbd7478 |
| institution | Directory Open Access Journal |
| issn | 2162-3945 2162-397X |
| language | English |
| last_indexed | 2024-12-17T03:02:41Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Adipocyte |
| spelling | doaj.art-f7604469915947ee89d7c648cfbd74782022-12-21T22:06:03ZengTaylor & Francis GroupAdipocyte2162-39452162-397X2020-01-019163664810.1080/21623945.2020.18386841838684Surface-associated lipid droplets: an intermediate site for lipid transport in human adipocytes?Björn Morén0Claes Fryklund1Karin Stenkula2Lund UniversityLund UniversityLund UniversityAdipose tissue plays a major role in regulating whole-body energy metabolism. While the biochemical processes regulating storage and release of excess energy are well known, the temporal organization of these events is much less defined. In this study, we have characterized the presence of small surface-associated lipid droplets, distinct from the central droplet, in primary human adipocytes. Based on microscopy analyses, we illustrate the distribution of mitochondria, endoplasmic reticulum and lysosomes in the vicinity of these specialized lipid droplets. Ultrastructure analysis confirmed the presence of small droplets in intact adipose tissue. Further, CIDEC, known to bind and regulate lipid droplet expansion, clearly localized at these lipid droplets. Neither acute or prolonged stimulation with insulin or isoprenaline, or pharmacologic intervention to suppress lipid flux, affected the presence of these lipid droplets. Still, phosphorylated perilipin and hormone-sensitive lipase accumulated at these droplets following adrenergic stimuli, which supports metabolic activity at these locations. Altogether, we propose these lipid droplet clusters represent an intermediate site involved in lipid transport in primary adipocytes.http://dx.doi.org/10.1080/21623945.2020.1838684lipid dropletadipocytesmitochondriaperilipintriglycerideslipolysis |
| spellingShingle | Björn Morén Claes Fryklund Karin Stenkula Surface-associated lipid droplets: an intermediate site for lipid transport in human adipocytes? Adipocyte lipid droplet adipocytes mitochondria perilipin triglycerides lipolysis |
| title | Surface-associated lipid droplets: an intermediate site for lipid transport in human adipocytes? |
| title_full | Surface-associated lipid droplets: an intermediate site for lipid transport in human adipocytes? |
| title_fullStr | Surface-associated lipid droplets: an intermediate site for lipid transport in human adipocytes? |
| title_full_unstemmed | Surface-associated lipid droplets: an intermediate site for lipid transport in human adipocytes? |
| title_short | Surface-associated lipid droplets: an intermediate site for lipid transport in human adipocytes? |
| title_sort | surface associated lipid droplets an intermediate site for lipid transport in human adipocytes |
| topic | lipid droplet adipocytes mitochondria perilipin triglycerides lipolysis |
| url | http://dx.doi.org/10.1080/21623945.2020.1838684 |
| work_keys_str_mv | AT bjornmoren surfaceassociatedlipiddropletsanintermediatesiteforlipidtransportinhumanadipocytes AT claesfryklund surfaceassociatedlipiddropletsanintermediatesiteforlipidtransportinhumanadipocytes AT karinstenkula surfaceassociatedlipiddropletsanintermediatesiteforlipidtransportinhumanadipocytes |