Nanostructured Surfaces to Promote Osteoblast Proliferation and Minimize Bacterial Adhesion on Titanium
The objective of this study was to investigate the potential of titanium nanotubes to promote the proliferation of human osteoblasts and to reduce monomicrobial biofilm adhesion. A secondary objective was to determine the effect of silicon carbide (SiC) on these nanostructured surfaces. Anodized tit...
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MDPI AG
2021-08-01
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| Online Access: | https://www.mdpi.com/1996-1944/14/16/4357 |
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| author | Samira Esteves Afonso Camargo Xinyi Xia Chaker Fares Fan Ren Shu-Min Hsu Dragos Budei Chairmandurai Aravindraja Lakshmyya Kesavalu Josephine F. Esquivel-Upshaw |
| author_facet | Samira Esteves Afonso Camargo Xinyi Xia Chaker Fares Fan Ren Shu-Min Hsu Dragos Budei Chairmandurai Aravindraja Lakshmyya Kesavalu Josephine F. Esquivel-Upshaw |
| author_sort | Samira Esteves Afonso Camargo |
| collection | DOAJ |
| description | The objective of this study was to investigate the potential of titanium nanotubes to promote the proliferation of human osteoblasts and to reduce monomicrobial biofilm adhesion. A secondary objective was to determine the effect of silicon carbide (SiC) on these nanostructured surfaces. Anodized titanium sheets with 100–150 nm nanotubes were either coated or not coated with SiC. After 24 h of osteoblast cultivation on the samples, cells were observed on all titanium sheets by SEM. In addition, the cytotoxicity was evaluated by CellTiter-BlueCell assay after 1, 3, and 7 days. The samples were also cultivated in culture medium with microorganisms incubated anaerobically with respective predominant periodontal bacteria viz. <i>Porphyromonas gingivalis</i>, <i>Treponema denticola</i>, and <i>Tannerella forsythia</i> as monoinfection at 37 °C for 30 days. The biofilm adhesion and coverage were evaluated through surface observation using Scanning Electron Microscopy (SEM). The results demonstrate that Ti nanostructured surfaces induced more cell proliferation after seven days. All groups presented no cytotoxic effects on human osteoblasts. In addition, SEM images illustrate that Ti nanostructured surfaces exhibited lower biofilm coverage compared to the reference samples. These results indicate that Ti nanotubes promoted osteoblasts proliferation and induced cell proliferation on the surface, compared with the controls. Ti nanotubes also reduced biofilm adhesion on titanium implant surfaces. |
| first_indexed | 2024-03-10T08:39:35Z |
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| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T08:39:35Z |
| publishDate | 2021-08-01 |
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| series | Materials |
| spelling | doaj.art-f760643e799e4b1ebfa7c07c96ffea502023-11-22T08:26:18ZengMDPI AGMaterials1996-19442021-08-011416435710.3390/ma14164357Nanostructured Surfaces to Promote Osteoblast Proliferation and Minimize Bacterial Adhesion on TitaniumSamira Esteves Afonso Camargo0Xinyi Xia1Chaker Fares2Fan Ren3Shu-Min Hsu4Dragos Budei5Chairmandurai Aravindraja6Lakshmyya Kesavalu7Josephine F. Esquivel-Upshaw8Department of Restorative Dental Sciences, Division of Prosthodontics, University of Florida College of Dentistry, Gainesville, FL 32610, USADepartment of Chemical Engineering, Herbert Wertheim College of Engineering, University of Florida, Gainesville, FL 32611, USADepartment of Chemical Engineering, Herbert Wertheim College of Engineering, University of Florida, Gainesville, FL 32611, USADepartment of Chemical Engineering, Herbert Wertheim College of Engineering, University of Florida, Gainesville, FL 32611, USADepartment of Restorative Dental Sciences, Division of Prosthodontics, University of Florida College of Dentistry, Gainesville, FL 32610, USADentix Millennium SRL, 087153 Giurgiu, RomaniaDepartment of Periodontology, College of Dentistry, University of Florida, Gainesville, FL 32611, USADepartment of Periodontology, College of Dentistry, University of Florida, Gainesville, FL 32611, USADepartment of Restorative Dental Sciences, Division of Prosthodontics, University of Florida College of Dentistry, Gainesville, FL 32610, USAThe objective of this study was to investigate the potential of titanium nanotubes to promote the proliferation of human osteoblasts and to reduce monomicrobial biofilm adhesion. A secondary objective was to determine the effect of silicon carbide (SiC) on these nanostructured surfaces. Anodized titanium sheets with 100–150 nm nanotubes were either coated or not coated with SiC. After 24 h of osteoblast cultivation on the samples, cells were observed on all titanium sheets by SEM. In addition, the cytotoxicity was evaluated by CellTiter-BlueCell assay after 1, 3, and 7 days. The samples were also cultivated in culture medium with microorganisms incubated anaerobically with respective predominant periodontal bacteria viz. <i>Porphyromonas gingivalis</i>, <i>Treponema denticola</i>, and <i>Tannerella forsythia</i> as monoinfection at 37 °C for 30 days. The biofilm adhesion and coverage were evaluated through surface observation using Scanning Electron Microscopy (SEM). The results demonstrate that Ti nanostructured surfaces induced more cell proliferation after seven days. All groups presented no cytotoxic effects on human osteoblasts. In addition, SEM images illustrate that Ti nanostructured surfaces exhibited lower biofilm coverage compared to the reference samples. These results indicate that Ti nanotubes promoted osteoblasts proliferation and induced cell proliferation on the surface, compared with the controls. Ti nanotubes also reduced biofilm adhesion on titanium implant surfaces.https://www.mdpi.com/1996-1944/14/16/4357nanotubesimplantosteoblastscoatingSiC |
| spellingShingle | Samira Esteves Afonso Camargo Xinyi Xia Chaker Fares Fan Ren Shu-Min Hsu Dragos Budei Chairmandurai Aravindraja Lakshmyya Kesavalu Josephine F. Esquivel-Upshaw Nanostructured Surfaces to Promote Osteoblast Proliferation and Minimize Bacterial Adhesion on Titanium Materials nanotubes implant osteoblasts coating SiC |
| title | Nanostructured Surfaces to Promote Osteoblast Proliferation and Minimize Bacterial Adhesion on Titanium |
| title_full | Nanostructured Surfaces to Promote Osteoblast Proliferation and Minimize Bacterial Adhesion on Titanium |
| title_fullStr | Nanostructured Surfaces to Promote Osteoblast Proliferation and Minimize Bacterial Adhesion on Titanium |
| title_full_unstemmed | Nanostructured Surfaces to Promote Osteoblast Proliferation and Minimize Bacterial Adhesion on Titanium |
| title_short | Nanostructured Surfaces to Promote Osteoblast Proliferation and Minimize Bacterial Adhesion on Titanium |
| title_sort | nanostructured surfaces to promote osteoblast proliferation and minimize bacterial adhesion on titanium |
| topic | nanotubes implant osteoblasts coating SiC |
| url | https://www.mdpi.com/1996-1944/14/16/4357 |
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