Performance of Colombian Silk Fibroin Hydrogels for Hyaline Cartilage Tissue Engineering
The development and evaluation of scaffolds play a crucial role in the engineering of hyaline cartilage tissue. This work aims to evaluate the performance of silk fibroin hydrogels fabricated from the cocoons of the Colombian hybrid in the in vitro regeneration of hyaline cartilage. The scaffolds we...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/13/4/297 |
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| author | Augusto Zuluaga-Vélez Carlos Andrés Toro-Acevedo Adrián Quintero-Martinez Jhon Jairo Melchor-Moncada Francisco Pedraza-Ordoñez Enrique Aguilar-Fernández Juan Carlos Sepúlveda-Arias |
| author_facet | Augusto Zuluaga-Vélez Carlos Andrés Toro-Acevedo Adrián Quintero-Martinez Jhon Jairo Melchor-Moncada Francisco Pedraza-Ordoñez Enrique Aguilar-Fernández Juan Carlos Sepúlveda-Arias |
| author_sort | Augusto Zuluaga-Vélez |
| collection | DOAJ |
| description | The development and evaluation of scaffolds play a crucial role in the engineering of hyaline cartilage tissue. This work aims to evaluate the performance of silk fibroin hydrogels fabricated from the cocoons of the Colombian hybrid in the in vitro regeneration of hyaline cartilage. The scaffolds were physicochemically characterized, and their performance was evaluated in a cellular model. The results showed that the scaffolds were rich in random coils and β-sheets in their structure and susceptible to various serine proteases with different degradation profiles. Furthermore, they showed a significant increase in <i>ACAN</i>, <i>COL10A1,</i> and <i>COL2A1</i> expression compared to pellet culture alone and allowed GAG deposition. The soluble portion of the scaffold did not affect chondrogenesis. Furthermore, they promoted the increase in <i>COL1A2</i>, showing a slight tendency to differentiate towards fibrous cartilage. The results also showed that Colombian silk could be used as a source of biomedical devices, paving the way for sericulture to become a more diverse economic activity in emerging countries. |
| first_indexed | 2024-03-09T16:15:24Z |
| format | Article |
| id | doaj.art-f764b9f253f94ef49b263f95f358b3d4 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-09T16:15:24Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-f764b9f253f94ef49b263f95f358b3d42023-11-24T15:51:01ZengMDPI AGJournal of Functional Biomaterials2079-49832022-12-0113429710.3390/jfb13040297Performance of Colombian Silk Fibroin Hydrogels for Hyaline Cartilage Tissue EngineeringAugusto Zuluaga-Vélez0Carlos Andrés Toro-Acevedo1Adrián Quintero-Martinez2Jhon Jairo Melchor-Moncada3Francisco Pedraza-Ordoñez4Enrique Aguilar-Fernández5Juan Carlos Sepúlveda-Arias6Grupo Infección e Inmunidad, Facultad de Ciencias de la Salud, Universidad Tecnológica de Pereira, Pereira 660003, ColombiaGrupo Infección e Inmunidad, Facultad de Ciencias de la Salud, Universidad Tecnológica de Pereira, Pereira 660003, ColombiaGrupo Infección e Inmunidad, Facultad de Ciencias de la Salud, Universidad Tecnológica de Pereira, Pereira 660003, ColombiaGrupo Infección e Inmunidad, Facultad de Ciencias de la Salud, Universidad Tecnológica de Pereira, Pereira 660003, ColombiaFacultad de Ciencias Agropecuarias, Universidad de Caldas, Manizales 170004, ColombiaGrupo Infección e Inmunidad, Facultad de Ciencias de la Salud, Universidad Tecnológica de Pereira, Pereira 660003, ColombiaGrupo Infección e Inmunidad, Facultad de Ciencias de la Salud, Universidad Tecnológica de Pereira, Pereira 660003, ColombiaThe development and evaluation of scaffolds play a crucial role in the engineering of hyaline cartilage tissue. This work aims to evaluate the performance of silk fibroin hydrogels fabricated from the cocoons of the Colombian hybrid in the in vitro regeneration of hyaline cartilage. The scaffolds were physicochemically characterized, and their performance was evaluated in a cellular model. The results showed that the scaffolds were rich in random coils and β-sheets in their structure and susceptible to various serine proteases with different degradation profiles. Furthermore, they showed a significant increase in <i>ACAN</i>, <i>COL10A1,</i> and <i>COL2A1</i> expression compared to pellet culture alone and allowed GAG deposition. The soluble portion of the scaffold did not affect chondrogenesis. Furthermore, they promoted the increase in <i>COL1A2</i>, showing a slight tendency to differentiate towards fibrous cartilage. The results also showed that Colombian silk could be used as a source of biomedical devices, paving the way for sericulture to become a more diverse economic activity in emerging countries.https://www.mdpi.com/2079-4983/13/4/297silk fibroinhydrogelshyaline cartilagetissue engineeringchondrogenesis |
| spellingShingle | Augusto Zuluaga-Vélez Carlos Andrés Toro-Acevedo Adrián Quintero-Martinez Jhon Jairo Melchor-Moncada Francisco Pedraza-Ordoñez Enrique Aguilar-Fernández Juan Carlos Sepúlveda-Arias Performance of Colombian Silk Fibroin Hydrogels for Hyaline Cartilage Tissue Engineering Journal of Functional Biomaterials silk fibroin hydrogels hyaline cartilage tissue engineering chondrogenesis |
| title | Performance of Colombian Silk Fibroin Hydrogels for Hyaline Cartilage Tissue Engineering |
| title_full | Performance of Colombian Silk Fibroin Hydrogels for Hyaline Cartilage Tissue Engineering |
| title_fullStr | Performance of Colombian Silk Fibroin Hydrogels for Hyaline Cartilage Tissue Engineering |
| title_full_unstemmed | Performance of Colombian Silk Fibroin Hydrogels for Hyaline Cartilage Tissue Engineering |
| title_short | Performance of Colombian Silk Fibroin Hydrogels for Hyaline Cartilage Tissue Engineering |
| title_sort | performance of colombian silk fibroin hydrogels for hyaline cartilage tissue engineering |
| topic | silk fibroin hydrogels hyaline cartilage tissue engineering chondrogenesis |
| url | https://www.mdpi.com/2079-4983/13/4/297 |
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