Estimating firing rates from calcium signals in locust projection neurons <i>in vivo</i>

Combining intracellular electrophysiology and multi-photon calcium imaging in vivo, we studied the relationship between calcium signals (sampled at 500-750 Hz) and spike output in principal neurons in the locust antennal lobe. Our goal was to determine whether the firing rate of individual neurons c...

Full description

Bibliographic Details
Main Authors: Laurent C Moreaux, Gilles Laurent
Format: Article
Language:English
Published: Frontiers Media S.A. 2007-11-01
Series:Frontiers in Neural Circuits
Subjects:
Online Access:http://journal.frontiersin.org/Journal/10.3389/neuro.04.002.2007/full
_version_ 1811314112499548160
author Laurent C Moreaux
Gilles Laurent
author_facet Laurent C Moreaux
Gilles Laurent
author_sort Laurent C Moreaux
collection DOAJ
description Combining intracellular electrophysiology and multi-photon calcium imaging in vivo, we studied the relationship between calcium signals (sampled at 500-750 Hz) and spike output in principal neurons in the locust antennal lobe. Our goal was to determine whether the firing rate of individual neurons can be estimated <I>in vivo</I> with calcium imaging and, if so, to measure directly the accuracy and resolution of our estimates. Using the calcium indicator Oregon Green BAPTA-1, we describe a simple method to reconstruct firing rates from dendritic calcium signals with 80-90% accuracy and 50ms temporal resolution.
first_indexed 2024-04-13T11:06:10Z
format Article
id doaj.art-f7661528075b4a289adc403b2cd631e6
institution Directory Open Access Journal
issn 1662-5110
language English
last_indexed 2024-04-13T11:06:10Z
publishDate 2007-11-01
publisher Frontiers Media S.A.
record_format Article
series Frontiers in Neural Circuits
spelling doaj.art-f7661528075b4a289adc403b2cd631e62022-12-22T02:49:16ZengFrontiers Media S.A.Frontiers in Neural Circuits1662-51102007-11-01110.3389/neuro.04.002.200772Estimating firing rates from calcium signals in locust projection neurons <i>in vivo</i>Laurent C Moreaux0Gilles Laurent1Division of Biology, California Institute of TechnologyDivision of Biology, California Institute of TechnologyCombining intracellular electrophysiology and multi-photon calcium imaging in vivo, we studied the relationship between calcium signals (sampled at 500-750 Hz) and spike output in principal neurons in the locust antennal lobe. Our goal was to determine whether the firing rate of individual neurons can be estimated <I>in vivo</I> with calcium imaging and, if so, to measure directly the accuracy and resolution of our estimates. Using the calcium indicator Oregon Green BAPTA-1, we describe a simple method to reconstruct firing rates from dendritic calcium signals with 80-90% accuracy and 50ms temporal resolution.http://journal.frontiersin.org/Journal/10.3389/neuro.04.002.2007/fullElectrophysiologyOlfactioncalcium imagingtwo-photon microscopyLocustOregon Green BAPTA-1
spellingShingle Laurent C Moreaux
Gilles Laurent
Estimating firing rates from calcium signals in locust projection neurons <i>in vivo</i>
Frontiers in Neural Circuits
Electrophysiology
Olfaction
calcium imaging
two-photon microscopy
Locust
Oregon Green BAPTA-1
title Estimating firing rates from calcium signals in locust projection neurons <i>in vivo</i>
title_full Estimating firing rates from calcium signals in locust projection neurons <i>in vivo</i>
title_fullStr Estimating firing rates from calcium signals in locust projection neurons <i>in vivo</i>
title_full_unstemmed Estimating firing rates from calcium signals in locust projection neurons <i>in vivo</i>
title_short Estimating firing rates from calcium signals in locust projection neurons <i>in vivo</i>
title_sort estimating firing rates from calcium signals in locust projection neurons i in vivo i
topic Electrophysiology
Olfaction
calcium imaging
two-photon microscopy
Locust
Oregon Green BAPTA-1
url http://journal.frontiersin.org/Journal/10.3389/neuro.04.002.2007/full
work_keys_str_mv AT laurentcmoreaux estimatingfiringratesfromcalciumsignalsinlocustprojectionneuronsiinvivoi
AT gilleslaurent estimatingfiringratesfromcalciumsignalsinlocustprojectionneuronsiinvivoi