Estimating firing rates from calcium signals in locust projection neurons <i>in vivo</i>
Combining intracellular electrophysiology and multi-photon calcium imaging in vivo, we studied the relationship between calcium signals (sampled at 500-750 Hz) and spike output in principal neurons in the locust antennal lobe. Our goal was to determine whether the firing rate of individual neurons c...
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Format: | Article |
Language: | English |
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Frontiers Media S.A.
2007-11-01
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Series: | Frontiers in Neural Circuits |
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Online Access: | http://journal.frontiersin.org/Journal/10.3389/neuro.04.002.2007/full |
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author | Laurent C Moreaux Gilles Laurent |
author_facet | Laurent C Moreaux Gilles Laurent |
author_sort | Laurent C Moreaux |
collection | DOAJ |
description | Combining intracellular electrophysiology and multi-photon calcium imaging in vivo, we studied the relationship between calcium signals (sampled at 500-750 Hz) and spike output in principal neurons in the locust antennal lobe. Our goal was to determine whether the firing rate of individual neurons can be estimated <I>in vivo</I> with calcium imaging and, if so, to measure directly the accuracy and resolution of our estimates. Using the calcium indicator Oregon Green BAPTA-1, we describe a simple method to reconstruct firing rates from dendritic calcium signals with 80-90% accuracy and 50ms temporal resolution. |
first_indexed | 2024-04-13T11:06:10Z |
format | Article |
id | doaj.art-f7661528075b4a289adc403b2cd631e6 |
institution | Directory Open Access Journal |
issn | 1662-5110 |
language | English |
last_indexed | 2024-04-13T11:06:10Z |
publishDate | 2007-11-01 |
publisher | Frontiers Media S.A. |
record_format | Article |
series | Frontiers in Neural Circuits |
spelling | doaj.art-f7661528075b4a289adc403b2cd631e62022-12-22T02:49:16ZengFrontiers Media S.A.Frontiers in Neural Circuits1662-51102007-11-01110.3389/neuro.04.002.200772Estimating firing rates from calcium signals in locust projection neurons <i>in vivo</i>Laurent C Moreaux0Gilles Laurent1Division of Biology, California Institute of TechnologyDivision of Biology, California Institute of TechnologyCombining intracellular electrophysiology and multi-photon calcium imaging in vivo, we studied the relationship between calcium signals (sampled at 500-750 Hz) and spike output in principal neurons in the locust antennal lobe. Our goal was to determine whether the firing rate of individual neurons can be estimated <I>in vivo</I> with calcium imaging and, if so, to measure directly the accuracy and resolution of our estimates. Using the calcium indicator Oregon Green BAPTA-1, we describe a simple method to reconstruct firing rates from dendritic calcium signals with 80-90% accuracy and 50ms temporal resolution.http://journal.frontiersin.org/Journal/10.3389/neuro.04.002.2007/fullElectrophysiologyOlfactioncalcium imagingtwo-photon microscopyLocustOregon Green BAPTA-1 |
spellingShingle | Laurent C Moreaux Gilles Laurent Estimating firing rates from calcium signals in locust projection neurons <i>in vivo</i> Frontiers in Neural Circuits Electrophysiology Olfaction calcium imaging two-photon microscopy Locust Oregon Green BAPTA-1 |
title | Estimating firing rates from calcium signals in locust projection neurons <i>in vivo</i> |
title_full | Estimating firing rates from calcium signals in locust projection neurons <i>in vivo</i> |
title_fullStr | Estimating firing rates from calcium signals in locust projection neurons <i>in vivo</i> |
title_full_unstemmed | Estimating firing rates from calcium signals in locust projection neurons <i>in vivo</i> |
title_short | Estimating firing rates from calcium signals in locust projection neurons <i>in vivo</i> |
title_sort | estimating firing rates from calcium signals in locust projection neurons i in vivo i |
topic | Electrophysiology Olfaction calcium imaging two-photon microscopy Locust Oregon Green BAPTA-1 |
url | http://journal.frontiersin.org/Journal/10.3389/neuro.04.002.2007/full |
work_keys_str_mv | AT laurentcmoreaux estimatingfiringratesfromcalciumsignalsinlocustprojectionneuronsiinvivoi AT gilleslaurent estimatingfiringratesfromcalciumsignalsinlocustprojectionneuronsiinvivoi |