Sterols and Sphingolipids as New Players in Cell Wall Building and Apical Growth of <i>Nicotiana tabacum</i> L. Pollen Tubes
Pollen tubes are tip-growing cells that create safe routes to convey sperm cells to the embryo sac for double fertilization. Recent studies have purified and biochemically characterized detergent-insoluble membranes from tobacco pollen tubes. These microdomains, called lipid rafts, are rich in stero...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-12-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/12/1/8 |
| _version_ | 1797406609481138176 |
|---|---|
| author | Nadia Stroppa Elisabetta Onelli Patrick Moreau Lilly Maneta-Peyret Valeria Berno Eugenia Cammarota Roberto Ambrosini Marco Caccianiga Monica Scali Alessandra Moscatelli |
| author_facet | Nadia Stroppa Elisabetta Onelli Patrick Moreau Lilly Maneta-Peyret Valeria Berno Eugenia Cammarota Roberto Ambrosini Marco Caccianiga Monica Scali Alessandra Moscatelli |
| author_sort | Nadia Stroppa |
| collection | DOAJ |
| description | Pollen tubes are tip-growing cells that create safe routes to convey sperm cells to the embryo sac for double fertilization. Recent studies have purified and biochemically characterized detergent-insoluble membranes from tobacco pollen tubes. These microdomains, called lipid rafts, are rich in sterols and sphingolipids and are involved in cell polarization in organisms evolutionarily distant, such as fungi and mammals. The presence of actin in tobacco pollen tube detergent-insoluble membranes and the preferential distribution of these domains on the apical plasma membrane encouraged us to formulate the intriguing hypothesis that sterols and sphingolipids could be a “trait d’union” between actin dynamics and polarized secretion at the tip. To unravel the role of sterols and sphingolipids in tobacco pollen tube growth, we used squalestatin and myriocin, inhibitors of sterol and sphingolipid biosynthesis, respectively, to determine whether lipid modifications affect actin fringe morphology and dynamics, leading to changes in clear zone organization and cell wall deposition, thus suggesting a role played by these lipids in successful fertilization. |
| first_indexed | 2024-03-09T03:27:58Z |
| format | Article |
| id | doaj.art-f76bb216efcf43a4a16407b02b7410ca |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T03:27:58Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-f76bb216efcf43a4a16407b02b7410ca2023-12-03T15:00:05ZengMDPI AGPlants2223-77472022-12-01121810.3390/plants12010008Sterols and Sphingolipids as New Players in Cell Wall Building and Apical Growth of <i>Nicotiana tabacum</i> L. Pollen TubesNadia Stroppa0Elisabetta Onelli1Patrick Moreau2Lilly Maneta-Peyret3Valeria Berno4Eugenia Cammarota5Roberto Ambrosini6Marco Caccianiga7Monica Scali8Alessandra Moscatelli9Dipartimento di Bioscienze, Università degli Studi di Milano, Via Celoria 26, 20133 Milan, ItalyDipartimento di Bioscienze, Università degli Studi di Milano, Via Celoria 26, 20133 Milan, ItalyCNRS, Laboratoire de Biogenèse Membranaire, University of Bordeaux, UMR 5200, 71 Avenue Edouard Bourlaux, 33140 Villenave d’Ornon, FranceCNRS, Laboratoire de Biogenèse Membranaire, University of Bordeaux, UMR 5200, 71 Avenue Edouard Bourlaux, 33140 Villenave d’Ornon, FranceALEMBIC Advanced Light and Electron Microscopy BioImaging Center, San Raffaele Scientific Institute, DIBIT 1, Via Olgettina 58, 20132 Milan, ItalyALEMBIC Advanced Light and Electron Microscopy BioImaging Center, San Raffaele Scientific Institute, DIBIT 1, Via Olgettina 58, 20132 Milan, ItalyDipartimento di Scienze e Politiche Ambientali, Università degli Studi di Milano, Via Celoria 26, 20133 Milan, ItalyDipartimento di Bioscienze, Università degli Studi di Milano, Via Celoria 26, 20133 Milan, ItalyDipartimento di Scienze della Vita, Università degli Studi di Siena, Via Aldo Moro 2, 53100 Siena, ItalyDipartimento di Bioscienze, Università degli Studi di Milano, Via Celoria 26, 20133 Milan, ItalyPollen tubes are tip-growing cells that create safe routes to convey sperm cells to the embryo sac for double fertilization. Recent studies have purified and biochemically characterized detergent-insoluble membranes from tobacco pollen tubes. These microdomains, called lipid rafts, are rich in sterols and sphingolipids and are involved in cell polarization in organisms evolutionarily distant, such as fungi and mammals. The presence of actin in tobacco pollen tube detergent-insoluble membranes and the preferential distribution of these domains on the apical plasma membrane encouraged us to formulate the intriguing hypothesis that sterols and sphingolipids could be a “trait d’union” between actin dynamics and polarized secretion at the tip. To unravel the role of sterols and sphingolipids in tobacco pollen tube growth, we used squalestatin and myriocin, inhibitors of sterol and sphingolipid biosynthesis, respectively, to determine whether lipid modifications affect actin fringe morphology and dynamics, leading to changes in clear zone organization and cell wall deposition, thus suggesting a role played by these lipids in successful fertilization.https://www.mdpi.com/2223-7747/12/1/8pollen tubeapical growth<i>Nicotiana tabacum</i>lipid nanodomainscell wallvesicle trafficking |
| spellingShingle | Nadia Stroppa Elisabetta Onelli Patrick Moreau Lilly Maneta-Peyret Valeria Berno Eugenia Cammarota Roberto Ambrosini Marco Caccianiga Monica Scali Alessandra Moscatelli Sterols and Sphingolipids as New Players in Cell Wall Building and Apical Growth of <i>Nicotiana tabacum</i> L. Pollen Tubes Plants pollen tube apical growth <i>Nicotiana tabacum</i> lipid nanodomains cell wall vesicle trafficking |
| title | Sterols and Sphingolipids as New Players in Cell Wall Building and Apical Growth of <i>Nicotiana tabacum</i> L. Pollen Tubes |
| title_full | Sterols and Sphingolipids as New Players in Cell Wall Building and Apical Growth of <i>Nicotiana tabacum</i> L. Pollen Tubes |
| title_fullStr | Sterols and Sphingolipids as New Players in Cell Wall Building and Apical Growth of <i>Nicotiana tabacum</i> L. Pollen Tubes |
| title_full_unstemmed | Sterols and Sphingolipids as New Players in Cell Wall Building and Apical Growth of <i>Nicotiana tabacum</i> L. Pollen Tubes |
| title_short | Sterols and Sphingolipids as New Players in Cell Wall Building and Apical Growth of <i>Nicotiana tabacum</i> L. Pollen Tubes |
| title_sort | sterols and sphingolipids as new players in cell wall building and apical growth of i nicotiana tabacum i l pollen tubes |
| topic | pollen tube apical growth <i>Nicotiana tabacum</i> lipid nanodomains cell wall vesicle trafficking |
| url | https://www.mdpi.com/2223-7747/12/1/8 |
| work_keys_str_mv | AT nadiastroppa sterolsandsphingolipidsasnewplayersincellwallbuildingandapicalgrowthofinicotianatabacumilpollentubes AT elisabettaonelli sterolsandsphingolipidsasnewplayersincellwallbuildingandapicalgrowthofinicotianatabacumilpollentubes AT patrickmoreau sterolsandsphingolipidsasnewplayersincellwallbuildingandapicalgrowthofinicotianatabacumilpollentubes AT lillymanetapeyret sterolsandsphingolipidsasnewplayersincellwallbuildingandapicalgrowthofinicotianatabacumilpollentubes AT valeriaberno sterolsandsphingolipidsasnewplayersincellwallbuildingandapicalgrowthofinicotianatabacumilpollentubes AT eugeniacammarota sterolsandsphingolipidsasnewplayersincellwallbuildingandapicalgrowthofinicotianatabacumilpollentubes AT robertoambrosini sterolsandsphingolipidsasnewplayersincellwallbuildingandapicalgrowthofinicotianatabacumilpollentubes AT marcocaccianiga sterolsandsphingolipidsasnewplayersincellwallbuildingandapicalgrowthofinicotianatabacumilpollentubes AT monicascali sterolsandsphingolipidsasnewplayersincellwallbuildingandapicalgrowthofinicotianatabacumilpollentubes AT alessandramoscatelli sterolsandsphingolipidsasnewplayersincellwallbuildingandapicalgrowthofinicotianatabacumilpollentubes |