The Influence of Prenatal Exposure to Methamphetamine on the Development of Dopaminergic Neurons in the Ventral Midbrain
Methamphetamine, a highly addictive central nervous system (CNS) stimulant, is used worldwide as an anorexiant and attention enhancer. Methamphetamine use during pregnancy, even at therapeutic doses, may harm fetal development. Here, we examined whether exposure to methamphetamine affects the morpho...
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2023-03-01
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author | Walaa F. Alsanie Sherin Abdelrahman Raed I. Felimban Heba A. Alkhatabi Ahmed Gaber Ebtisam Abdulah Alosimi Majid Alhomrani Hamza Habeeballah Charlotte A. E. Hauser Abdulhakeem S. Alamri Aiysha Althobaiti Abdulaziz Alsharif Ahmed S. Alzahrani Mohammad S. Al-Ghamdi Bassem M. Raafat Khaled A. Alswat Yusuf S. Althobaiti Yousif A. Asiri |
author_facet | Walaa F. Alsanie Sherin Abdelrahman Raed I. Felimban Heba A. Alkhatabi Ahmed Gaber Ebtisam Abdulah Alosimi Majid Alhomrani Hamza Habeeballah Charlotte A. E. Hauser Abdulhakeem S. Alamri Aiysha Althobaiti Abdulaziz Alsharif Ahmed S. Alzahrani Mohammad S. Al-Ghamdi Bassem M. Raafat Khaled A. Alswat Yusuf S. Althobaiti Yousif A. Asiri |
author_sort | Walaa F. Alsanie |
collection | DOAJ |
description | Methamphetamine, a highly addictive central nervous system (CNS) stimulant, is used worldwide as an anorexiant and attention enhancer. Methamphetamine use during pregnancy, even at therapeutic doses, may harm fetal development. Here, we examined whether exposure to methamphetamine affects the morphogenesis and diversity of ventral midbrain dopaminergic neurons (VMDNs). The effects of methamphetamine on morphogenesis, viability, the release of mediator chemicals (such as ATP), and the expression of genes involved in neurogenesis were evaluated using VMDNs isolated from the embryos of timed-mated mice on embryonic day 12.5. We demonstrated that methamphetamine (10 µM; equivalent to its therapeutic dose) did not affect the viability and morphogenesis of VMDNs, but it reduced the ATP release negligibly. It significantly downregulated <i>Lmx1a</i>, <i>En1</i>, <i>Pitx3</i>, <i>Th</i>, <i>Chl1</i>, <i>Dat</i>, and <i>Drd1</i> but did not affect <i>Nurr1</i> or <i>Bdnf</i> expression. Our results illustrate that methamphetamine could impair VMDN differentiation by altering the expression of important neurogenesis-related genes. Overall, this study suggests that methamphetamine use may impair VMDNs in the fetus if taken during pregnancy. Therefore, it is essential to exercise strict caution for its use in expectant mothers. |
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spelling | doaj.art-f76f07a47a1b49b28575ad7e2a94e9b52023-11-17T11:37:19ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01246566810.3390/ijms24065668The Influence of Prenatal Exposure to Methamphetamine on the Development of Dopaminergic Neurons in the Ventral MidbrainWalaa F. Alsanie0Sherin Abdelrahman1Raed I. Felimban2Heba A. Alkhatabi3Ahmed Gaber4Ebtisam Abdulah Alosimi5Majid Alhomrani6Hamza Habeeballah7Charlotte A. E. Hauser8Abdulhakeem S. Alamri9Aiysha Althobaiti10Abdulaziz Alsharif11Ahmed S. Alzahrani12Mohammad S. Al-Ghamdi13Bassem M. Raafat14Khaled A. Alswat15Yusuf S. Althobaiti16Yousif A. Asiri17Department of Clinical Laboratories Sciences, The Faculty of Applied Medical Sciences, Taif University, Taif 21944, Saudi ArabiaLaboratory for Nanomedicine, Division of Biological and Environmental Science and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955, Saudi ArabiaDepartment of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah 21589, Saudi ArabiaCentre of Biomedical Sciences Research (CBSR), Deanship of Scientific Research, Taif University, Taif 21944, Saudi ArabiaCentre of Biomedical Sciences Research (CBSR), Deanship of Scientific Research, Taif University, Taif 21944, Saudi ArabiaDepartment of Clinical Laboratories Sciences, The Faculty of Applied Medical Sciences, Taif University, Taif 21944, Saudi ArabiaDepartment of Medical Laboratory Technology, Faculty of Applied Medical Sciences in Rabigh, King Abdulaziz University, Jeddah 21589, Saudi ArabiaLaboratory for Nanomedicine, Division of Biological and Environmental Science and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955, Saudi ArabiaDepartment of Clinical Laboratories Sciences, The Faculty of Applied Medical Sciences, Taif University, Taif 21944, Saudi ArabiaCentre of Biomedical Sciences Research (CBSR), Deanship of Scientific Research, Taif University, Taif 21944, Saudi ArabiaDepartment of Clinical Laboratories Sciences, The Faculty of Applied Medical Sciences, Taif University, Taif 21944, Saudi ArabiaCentre of Biomedical Sciences Research (CBSR), Deanship of Scientific Research, Taif University, Taif 21944, Saudi ArabiaDepartment of Clinical Laboratories Sciences, The Faculty of Applied Medical Sciences, Taif University, Taif 21944, Saudi ArabiaDepartment of Radiological Sciences, College of Applied Medical Sciences, Taif University, Taif 21944, Saudi ArabiaDepartment of Internal Medicine, School of Medicine, Taif University, Taif 21944, Saudi ArabiaDepartment of Pharmacology and Toxicology, College of Pharmacy, Taif University, Taif 21944, Saudi ArabiaDepartment of Clinical Pharmacy, College of Pharmacy, Taif University, Taif 21944, Saudi ArabiaMethamphetamine, a highly addictive central nervous system (CNS) stimulant, is used worldwide as an anorexiant and attention enhancer. Methamphetamine use during pregnancy, even at therapeutic doses, may harm fetal development. Here, we examined whether exposure to methamphetamine affects the morphogenesis and diversity of ventral midbrain dopaminergic neurons (VMDNs). The effects of methamphetamine on morphogenesis, viability, the release of mediator chemicals (such as ATP), and the expression of genes involved in neurogenesis were evaluated using VMDNs isolated from the embryos of timed-mated mice on embryonic day 12.5. We demonstrated that methamphetamine (10 µM; equivalent to its therapeutic dose) did not affect the viability and morphogenesis of VMDNs, but it reduced the ATP release negligibly. It significantly downregulated <i>Lmx1a</i>, <i>En1</i>, <i>Pitx3</i>, <i>Th</i>, <i>Chl1</i>, <i>Dat</i>, and <i>Drd1</i> but did not affect <i>Nurr1</i> or <i>Bdnf</i> expression. Our results illustrate that methamphetamine could impair VMDN differentiation by altering the expression of important neurogenesis-related genes. Overall, this study suggests that methamphetamine use may impair VMDNs in the fetus if taken during pregnancy. Therefore, it is essential to exercise strict caution for its use in expectant mothers.https://www.mdpi.com/1422-0067/24/6/5668methamphetaminedopaminergic neuronsfetal neurodevelopmentventral midbrainembryonic neurons |
spellingShingle | Walaa F. Alsanie Sherin Abdelrahman Raed I. Felimban Heba A. Alkhatabi Ahmed Gaber Ebtisam Abdulah Alosimi Majid Alhomrani Hamza Habeeballah Charlotte A. E. Hauser Abdulhakeem S. Alamri Aiysha Althobaiti Abdulaziz Alsharif Ahmed S. Alzahrani Mohammad S. Al-Ghamdi Bassem M. Raafat Khaled A. Alswat Yusuf S. Althobaiti Yousif A. Asiri The Influence of Prenatal Exposure to Methamphetamine on the Development of Dopaminergic Neurons in the Ventral Midbrain International Journal of Molecular Sciences methamphetamine dopaminergic neurons fetal neurodevelopment ventral midbrain embryonic neurons |
title | The Influence of Prenatal Exposure to Methamphetamine on the Development of Dopaminergic Neurons in the Ventral Midbrain |
title_full | The Influence of Prenatal Exposure to Methamphetamine on the Development of Dopaminergic Neurons in the Ventral Midbrain |
title_fullStr | The Influence of Prenatal Exposure to Methamphetamine on the Development of Dopaminergic Neurons in the Ventral Midbrain |
title_full_unstemmed | The Influence of Prenatal Exposure to Methamphetamine on the Development of Dopaminergic Neurons in the Ventral Midbrain |
title_short | The Influence of Prenatal Exposure to Methamphetamine on the Development of Dopaminergic Neurons in the Ventral Midbrain |
title_sort | influence of prenatal exposure to methamphetamine on the development of dopaminergic neurons in the ventral midbrain |
topic | methamphetamine dopaminergic neurons fetal neurodevelopment ventral midbrain embryonic neurons |
url | https://www.mdpi.com/1422-0067/24/6/5668 |
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